Extract and method for extracting tea saponin from oil-tea seed residue

A technology of camellia oleifera seed cake and extraction method, which is applied in the fields of chemical instruments and methods, preparation of sugar derivatives, steroids, etc., and can solve problems such as difficult to meet industrial production, production process, complex equipment, and low yield of tea saponin , to achieve the effect of low equipment requirements, simple method, and easy industrial production

Inactive Publication Date: 2011-02-09
HUAZHONG AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the yield of tea saponin extracted by these methods is not high, and the production process and equipment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Take 2g of the pretreated camellia seed cake, pass through a 60-mesh sieve, and then add an extract to it, which is an alkaline ethanol solution with pH=9, wherein the volume percentage concentration of ethanol is 60%, and the alkali used is hydrogen Sodium oxide, the rest is water, the mass ratio of the sieved camellia seed cake to the extract is 1:10, and then leached at 60°C for 2h. Centrifuge to remove the precipitate (i.e. the remaining camellia seed cake after leaching), to obtain an ethanol extract containing tea saponin. The yield of tea saponin was 16.47% by using the vanillin-concentrated sulfuric acid method for colorimetric determination at 550 nm.

Embodiment 2

[0018] Take 2g of the pretreated camellia seed cake, pass through a 60-mesh sieve, and then add an extract to it, which is an alkaline ethanol solution with pH=9, wherein the volume percentage concentration of ethanol is 60%, and the alkali used is hydrogen Sodium oxide, the rest is water, the mass ratio of the sieved camellia seed cake to the extract is 1:15, and then leached at 60°C for 2h. Centrifuge and remove the precipitate to obtain an ethanol extract containing tea saponin. The yield of tea saponin was 17.68% by colorimetric determination at 550nm by vanillin-concentrated sulfuric acid method.

Embodiment 3

[0020] Take 2g of the pretreated camellia seed cake, pass through a 60-mesh sieve, and then add an extract to it, which is an alkaline ethanol solution with pH=9, wherein the volume percentage concentration of ethanol is 60%, and the alkali used is hydrogen Sodium oxide, the rest is water, the mass ratio of the sieved camellia seed cake to the extract is 1:20, and then leached at 60°C for 2h. Centrifuge and remove the precipitate to obtain an ethanol extract containing tea saponin. The yield of tea saponin was 18.12% by using the vanillin-concentrated sulfuric acid method for colorimetric determination at 550 nm.

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PUM

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Abstract

The invention discloses extract and method for extracting tea saponin from oil-tea seed residue. The extract is an alkaline ethanol solution with pH value of 8-13, the alkali is sodium hydroxide or potassium hydroxide, the volume percentage concentration of the ethanol is 50-90%, and the balance is water. The method comprises the steps of: smashing and drying the oil-tea seed residue, degreasing, drying and screening, then extracting by using the extract at a temperature of 40-95 DEG C for 2-7 hours, centrifugally separating, and removing the deposit to obtain the ethanol extract including the tea saponin. By adopting the extract and the method of the invention, the tea saponin in the oil-tea seed residue can be sufficiently extracted with higher yield; and the method is simple and easy to implement.

Description

technical field [0001] The invention relates to an extraction solution and an extraction method for extracting tea saponin from camellia seed cake, belonging to the field of agricultural products and food processing. Background technique [0002] Tea saponin (Tea saponin), also known as tea saponin, tea saponin, is a class of natural glycoside compounds contained in the Theaceae plants (such as tea, camellia, camellia oleifera). 30 h 50 o 6 ) is a pentacyclic triterpenoid saponin composed of angelic acid and sugar bodies (galactose, arabinose, glucuronic acid). Widely present in the roots, stems, leaves, flowers and fruits of the Theaceae plants. In 1931, Japanese scholar Aoyama Jiro extracted tea saponin for the first time, and in 1952, Shigao Moriyama and Ueda Yangcai of Tokyo University in Japan separated and obtained tea saponin pure crystals. As early as the 1950s, my country began to study the extraction process of tea saponin. In 1979, the industrial test of separ...

Claims

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Application Information

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IPC IPC(8): C07J63/00C07H1/08C07H15/256
Inventor 王承明李敏
Owner HUAZHONG AGRI UNIV
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