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Molecular pathological classification method of diffuse large B-cell lymphomata, kit and application thereof

A technology of pathological typing and B cells, which is applied in the field of hematology oncology and medicine, can solve the problems of low efficiency and cumbersome operation, and achieve the effects of good repeatability, improved work efficiency and reduced cost

Inactive Publication Date: 2011-02-09
SHANGHAI BIOCHIP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The present invention aims to solve the technical problems of cumbersome operation and low efficiency when performing molecular pathological typing of diffuse large B-cell lymphoma by ordinary immunohistochemical methods, and provides a molecular pathological typing method for diffuse large B-cell lymphoma combined with quantum dot technology. type method, significantly improving the efficiency of the experiment

Method used

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  • Molecular pathological classification method of diffuse large B-cell lymphomata, kit and application thereof
  • Molecular pathological classification method of diffuse large B-cell lymphomata, kit and application thereof
  • Molecular pathological classification method of diffuse large B-cell lymphomata, kit and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1 Quantum dot immunohistochemical detection of diffuse large B-cell lymphoma

[0039] 1. Specimen collection

[0040] All DLBCL specimens were fixed with 10% neutral formaldehyde, routinely dehydrated, embedded in paraffin, stained with hematoxylin-eosin, and diagnosed according to the new WHO classification by doctors specialized in lymphoma research.

[0041] 2. Specimen Processing

[0042] Immerse paraffin sections that have been dried in the oven twice in xylene for 15 minutes. Take out the slices and put them in 100% absolute ethanol for 10 minutes twice; put them in 90%-70% ethanol at different levels for 10 minutes each. Take out and place in distilled water. Take out the slices in distilled water, shake off and dry the liquid around the tissue on the slices, place them flat in a wet box, add peroxidase blocker-3% methanol-H dropwise 2 o 2 Incubate on tissue in the dark for 20 minutes. Rinse with distilled water, then place the slices in TBS buffer,...

Embodiment 2

[0068] Example 2 Quantum dot immunohistochemical detection of diffuse large B-cell lymphoma on a tissue chip

[0069] 1. Specimen Processing

[0070] Before dewaxing, place the tissue chips in a 60°C thermostat and bake for 30 minutes; soak in xylene I for 10 minutes, soak in xylene II for 10 minutes, soak in xylene III for 10 minutes, and soak in xylene IV for 10 minutes. Soak for 10 minutes; soak in absolute ethanol I for 5 minutes, then soak in absolute ethanol II for 5 minutes; soak in 95% ethanol for 5 minutes; soak in 85% ethanol for 5 minutes; soak in 70% ethanol for 5 minutes; distilled water Soak for 5 minutes.

[0071] Antigen heat retrieval:

[0072] Take a certain amount of 0.01M citrate buffer pH 6.0 in a pressure cooker and heat it to boiling over high heat. Place the dewaxed and hydrated tissue chip on a high-temperature-resistant plastic slice rack and put it into the boiling buffer, cover it Put the lid on the pressure valve and continue to heat until the a...

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Abstract

The invention discloses a molecular pathological classification method of diffuse large B-cell lymphomata, which use three quantum dot markers of different wavelengths as fluorescent probes to detect the expression of CD10, Bcl-6 and MUM1 proteins in a biological sample. The invention also discloses a kit for the molecular pathological classification of diffuse large B-cell lymphomata and an application thereof. The molecular pathological classification method of diffuse large B-cell lymphomata obviously improves the working efficiency, and can provide a rational regimen for patients with diffuse large B-cell lymphomata, thereby being beneficial to saving medical expenses.

Description

technical field [0001] The invention relates to the fields of hemato-oncology and medicine, in particular to a molecular pathological typing method, kit and application of diffuse large B-cell lymphoma. Background technique [0002] Diffuse large B-cell lymphoma (DLBCL) is the most common non-Hodgkin's lymphoma, accounting for about 40% of newly diagnosed non-Hodgkin's lymphoma each year. The current incidence of non-Hodgkin's lymphoma in my country is about 3-4 / 100,000, and it is increasing at a rate of 2-3% per year, among which diffuse large B-cell lymphoma (DLBCL) is the most common, accounting for about 50% of tumors, therefore, the annual national new cases are about 20,000-30,000. [0003] DLBCL has significant biological heterogeneity, and patients have significant differences in response to treatment. The conventional CHOP combined chemotherapy regimen (cyclophosphamide, doxorubicin, vincristine and prednisone) can only make about 40% of patients Get long-term reli...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/577
Inventor 宋凯张庆华李军民
Owner SHANGHAI BIOCHIP
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