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Method for preparing general flavone in star-of-Bethlehem and application thereof to antitumor, anti-inflammatory and analgesic medicines

A technology of evergreen and total flavonoids, applied in the field of medicine, can solve the problem of low proportion of total flavonoid extract components

Active Publication Date: 2011-02-16
吉林天力泰药业有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of this invention is to provide a kind of preparation method of the total flavonoids of tiger eye evergreen, overcome the deficiency that the total flavonoids of the existing extract method of tiger eye evergreen active ingredient is low in the proportion of extract components

Method used

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  • Method for preparing general flavone in star-of-Bethlehem and application thereof to antitumor, anti-inflammatory and analgesic medicines
  • Method for preparing general flavone in star-of-Bethlehem and application thereof to antitumor, anti-inflammatory and analgesic medicines
  • Method for preparing general flavone in star-of-Bethlehem and application thereof to antitumor, anti-inflammatory and analgesic medicines

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Dried Evergreen 1.0kg, crushed into 20 mesh coarse powder, cold-soaked twice with 80% ethanol (volume ratio concentration), 15 days each time, combined the soaking liquid, concentrated under reduced pressure to form a thick paste (relative density 1.2, 70°C) , add 600-800 milliliters of water and stir, cool down and precipitate. Place in a centrifuge (1200-1600rad / min) to settle and centrifuge for 15-30 minutes, and take the supernatant for later use.

[0060] Take the macroporous adsorption resin, pack it into a column at a ratio of 1:2 to the weight ratio of the crude drug, load the above-mentioned spare supernatant, first elute with 2-4 times the column volume of deionized water, and then use 4-6 times the column volume Volume 20%-60% ethanol (volume ratio) carries out gradient elution, collects the ethanol eluent, 50-70 ℃ decompression concentration, 50-70 ℃ drying, obtains the total flavonoid extract of tiger eye evergreen, yield (weight ratio ) is 2.7%.

Embodiment 2

[0061] Embodiment 2: 1.0kg of dried Dieffenbachia tiger eye is pulverized into a coarse powder (20 orders), heated and refluxed with 80% ethanol (volume ratio) to extract 2 times, the combined extracts are concentrated under reduced pressure to become a thick paste (relative density 1.2, 70°C ), add 600-800 milliliters of water and stir, cooling and precipitation. Place in a centrifuge (1200-1600rad / min) to settle and centrifuge for 15-30 minutes, and take the supernatant for later use.

[0062] Take the macroporous adsorption resin, pack it into a column at a ratio of 1:1 to the weight ratio of the crude drug, load the above-mentioned spare supernatant, first elute with 2-4 times the column volume of deionized water, and then use 4-6 times the column volume Carry out gradient elution with 20%-70% ethanol by volume, collect the ethanol eluate, concentrate under reduced pressure at 50-70°C, and dry at 50-70°C to obtain the total flavonoids extract of Dieffenbachia tiger eye, wi...

Embodiment 3

[0063] Example 3: Dried Evergreen 1.0kg is pulverized into coarse powder (20 orders), soaked with 60% ethanol (volume ratio) after 48 hours, extracts by percolation, and percolates until the color of the effluent is light, and the flavonoid reaction is not obvious. Concentrate under reduced pressure to form a thick paste (relative density 1.2, 70°C), add 600-800 ml of water to stir, and cool to precipitate. Place in a centrifuge (1200-1600rad / min) to settle and centrifuge for 15-30 minutes, and take the supernatant for later use.

[0064] Take the macroporous adsorption resin, pack it into a column at a ratio of 1:1 to the weight ratio of the crude drug, load the above-mentioned supernatants, first elute with 2-4 times the column volume of deionized water, and then use 4-6 times the column volume The column volume is 30%-80% ethanol for gradient elution. Collect the ethanol eluate. Concentrate under reduced pressure at 50-70°C and dry at 50-70°C to obtain the total flavonoids...

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Abstract

The invention relates to a method for preparing general flavone in star-of-Bethlehem and application thereof to antitumor, anti-inflammatory and analgesic medicines, which belongs to the field of medicines. The method overcomes the disadvantage of low proportion of general flavone in components of an extract in the traditional extraction method. The preparation method comprises the following steps of: drying and pulverizing star-of-Bethlehem; carrying out reflux extraction or dipping extraction or percolation extraction by using alcohol or methanol, blending the extracting solutions, and decompressing and concentrating the blended extracting solution; adding water to the concentrated solution, precipitating and centrifuging; separating the centrifugate with macroporous absorbent resin or a polyamide column; eluting with water or diluted ethanol, and eliminating impurities; carrying out gradient elution with ethanol; collecting eluent, decompressing, concentrating, drying and pulverizing to obtain a general flavone extract of star-of-Bethlehem. The invention has the advantages that the effective ingredients of star-of-Bethlehem are screened, the active parts with antitumor, anti-inflammatory and analgesic activity are identified, and the preparation method of general flavone in star-of-Bethlehem is determined. A traditional Chinese medicine preparation, without toxic and side effect and using the general flavone in star-of-Bethlehem as the raw material, is obtained. The invention provides a new selection for antitumor, anti-inflammation and analgesic for clinic.

Description

Technical field: [0001] The invention belongs to the field of medicine, and in particular relates to a method for extracting and preparing total flavonoids from the traditional Chinese medicine Dieffenbachia tiger eye. The total flavonoids extracted from Dieffenbachia tiger eye are anti-inflammatory in treating various cancers and various inflammatory lesions of various systems, organs and tissues of the human body. Analgesic and Trauma, Surgery, Fracture, Cancer Pain Drug Application. Background technique: [0002] Evergreen (Omithogalum caudatum Ait), also known as pearl grass, evergreen, squill, gourd orchid, is a perennial plant of the genus Omithogalum caudatum Ait in the family Liliaceae. Mainly distributed in the temperate regions of the Eastern Hemisphere, it is native to southern Africa and cultivated in northern my country. The medicine is sweet in taste and slightly cold, and returns to the Liver and Spleen Channels. It has the functions of clearing away heat an...

Claims

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Application Information

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IPC IPC(8): A61P35/00A61K36/896A61P29/00
Inventor 孙佳明高陆修涞贵
Owner 吉林天力泰药业有限公司
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