Walnut shell extract and anti-HIV pharmaceutical application thereof

A technology for HIV and walnut shells, applied in the field of medicine, can solve problems such as non-reuse, and achieve the effects of abundant natural resources, stable ingredients and efficacy, and abundant and easy-to-obtain resources.

Inactive Publication Date: 2011-03-02
DALI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the discarded walnut shells ar...

Method used

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  • Walnut shell extract and anti-HIV pharmaceutical application thereof
  • Walnut shell extract and anti-HIV pharmaceutical application thereof
  • Walnut shell extract and anti-HIV pharmaceutical application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1 : Preparation of walnut shell extract A

[0018] Take 1 kg of crude walnut shell, crush it, add 800 ml of sodium hydroxide solution (1 mol / liter), soak overnight at room temperature, and filter. Add 700 milliliters of sodium hydroxide solution (1 mol / liter) again, stand for 2 hours, filter, and combine the filtrates. Add glacial acetic acid to the filtrate to pH=5, let stand for 1 hour, and filter. Add 1 times the amount of ethanol (95%) to the filtrate, let it stand for 1 hour, and filter. Add 1 times the amount of ethanol (95%) to the filtrate, let it stand for 1 hour, and filter to obtain the precipitate a. Put a at 40°C under reduced pressure, and dry it for 2 hours to obtain 1.8 g of dry product, that is, Product A. Extraction rate: 0.18%.

Embodiment 2

[0019] Example 2 : Preparation of walnut shell extract B

[0020] Take 1 kg of crude walnut shell, crush it, add 800 ml of sodium hydroxide solution (1 mol / liter), soak overnight at room temperature, and filter. Add 700 milliliters of sodium hydroxide solution (1 mol / liter) again, stand for 2 hours, filter, and combine the filtrates. Add glacial acetic acid to the filtrate to pH=5, let stand for 1 hour, and filter. Add 1 times the amount of ethanol (95%) to the filtrate, let it stand for 1 hour, and filter. Add 1 times the amount of ethanol (95%) to the filtrate, let it stand for 1 hour, and filter. Continue to add 3 times the amount of ethanol (95%) to the filtrate, let it stand for 1 hour, and filter to obtain precipitate b. Place b at 40°C under reduced pressure, and dry for 2 hours to obtain 1.0 g of dry product, that is, product B. Extraction rate: 0.1%.

Embodiment 3

[0021] Example 3 : Activity test of walnut shell extract A inhibiting HIV virus

[0022]3.1 Experimental principle and basis: According to the "Technical Guidelines for Non-clinical Pharmacodynamics Research of Anti-HIV Drugs (2006)" issued by the State Food and Drug Administration (SFDA), the anti-HIV-1 activity of the drug was tested using international general experimental methods. detection.

[0023] 3.2 Materials and methods

[0024] 3.2.1 Determination of drugs and compounds: the sample to be tested is the walnut shell extract A prepared according to Example 1 (hereinafter referred to as "sample 1"). The sample to be tested was dissolved in DMSO, stored at 4°C, and the storage concentration was 25.0 mg / ml.

[0025] 3.2.2 Reagents and solutions: biological buffer HEPES (N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid), thiazolyl blue MTT (3-(4,5-Dimethylthiazol-2-yl)- 2,5-diphenyl tetrazolium bromide), DMF (N, N-dimethylformamide), penicillin (Penicillin), strept...

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Abstract

The invention provides a walnut shell extract and anti-HIV pharmaceutical application thereof, in particular to relating to an extraction and separation method of effective parts of a walnut shell and medical application thereof. The walnut shell extract is prepared by soaking the crushed walnut shell with alkaline solution, extracting, performing alcohol precipitation, recovering a solvent and drying. The obtained walnut shell extract can effectively inhibit HIV (human immunodeficiency virus) and is expected to be developed into a drug for preventing and treating AIDS (acquired immure deficiency syndrome). Pharmacodynamics test results show that the walnut shell has in vitro anti-HIV-1 activity, and therapeutic index thereof is more than 400.

Description

technical field [0001] The invention relates to the technical field of medicine. Specifically, the present invention relates to a walnut shell extract and its medical use. The extract exhibits effective function of inhibiting HIV in vitro, has a high therapeutic index, and can be expected to be developed into a drug for preventing and treating AIDS. Background technique [0002] The full medical name of AIDS is "Acquired Immune Deficiency Syndrome" (Acquired Immune Deficiency Syndrome, AIDS), which is caused by human immunodeficiency virus (HIV, also known as AIDS virus). HIV virus is a virus that can attack the human immune system. It targets the most important T4 lymphocytes in the human immune system, engulfs and destroys T4 lymphocytes in large quantities, thereby destroying the human immune system and eventually causing the immune system to collapse. It makes the human body fall ill due to the loss of resistance to various diseases, resulting in various infections or ...

Claims

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Application Information

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IPC IPC(8): A61K36/52A61P31/18A61K131/00
Inventor 刘光明吕永俊李好枝周萍张海珠赵昱罗建蓉张桢李冬梅杨敏张兰胜李春艳董光平
Owner DALI UNIV
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