New application of polysaccharides from fructus rosae roxburghii for protection against ionizing radiation
A technology of Rosa roxburghii polysaccharides and γ-rays, which is applied in the field of medicine, can solve problems that have not been reported in the prior art, and achieve the effects of broad medicinal prospects, wide sources, and easy-to-obtain raw materials
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Embodiment 1
[0017] [Example 1] Cytotoxicity detection of Rosa roxburghii polysaccharides on V79 cells
[0018] Cell characteristics: Chinese hamster lung fibroblast V79, adherent growth, doubling time 12-14 hours.
[0019] 1. Culture conditions
[0020] Cells were cultured in DMEM medium (containing 10% FBS, 100 U / mL penicillin and 100 U / mL streptomycin), 37°C, 5% CO 2 Incubator, replace the culture medium every 2-3 days, the recommended cell culture density is 1-2×10 5 / mL.
[0021] 2. Drug preparation
[0022] Dissolve the Rosa roxburghii polysaccharide in DMEM culture solution, ultrasonically assist in dissolving, and prepare a stock solution with a storage concentration of 10 mg / ml for future use.
[0023] 3. Toxicity testing
[0024] Take V79 cells in the logarithmic growth phase (1×10 5 each / mL) were inoculated in a 96-well culture plate, 100 μL / hole, 100 μL of medicine in each well, so that the final concentration of the medicine was 2000, 1000, 500, 100, 50, 25, 12.5 μg / mL (...
Embodiment 2
[0025] [Embodiment two] Rosa roxburghii polysaccharide pair 60 C 0 Protective effect of loss of viability of V79 cells induced by γ-rays
[0026] 1. The ionizing radiation protection effect of different concentrations of drugs on V79 cells
[0027] Take V79 cells in the logarithmic growth phase (1×10 5 1 / mL) were inoculated in a 96-well culture plate, 100 μL / well, 100 μL of drug per well, so that the final drug concentration was 400, 200, 100, 50, 25, 12.5 μg / mL, 6 wells for each concentration, and a blank control group was set up Cell suspension, cell suspension of irradiation group. After 24 hours of drug action, 5Gy was irradiated. 24 hours after irradiation, 10 μL of CCK-8 solution was added to each well, and the culture was continued for 4 hours. The absorbance value of each well was measured at 490 nm in a multifunctional microplate reader. Compared with the irradiation group: #, P60 The loss of viability of V79 cells irradiated with 5Gy of COγ rays showed a dose-dep...
Embodiment 3
[0031] [Embodiment three] Rosa roxburghii polysaccharide pair 60 C 0 The effect of 8Gy γ-ray whole-body irradiation on the survival rate of Kunming mice
[0032] Animal grouping, dosing and irradiation:
[0033] After adapting to the environment, male Kunming mice were divided into normal group, irradiation group, positive drug group and drug group according to needs, with 10 mice in each group. The normal group and the irradiation group were given distilled water by intragastric administration, the drug group was given the corresponding drug by intragastric administration for 6 consecutive days, and the positive drug group was given "523" once by intragastric administration 24 hours before the irradiation, with a dose of 5 mg / kg, and the whole body was irradiated once after administration. , the dose was 8Gy, and the patients were continuously observed for 30 days after exposure. The results showed that the 30-day survival rate of mice given 100mg / kg Rosa roxburghii polysa...
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