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New application of polysaccharides from fructus rosae roxburghii for protection against ionizing radiation

A technology of Rosa roxburghii polysaccharides and γ-rays, which is applied in the field of medicine, can solve problems that have not been reported in the prior art, and achieve the effects of broad medicinal prospects, wide sources, and easy-to-obtain raw materials

Inactive Publication Date: 2012-05-30
XINXIANG MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The invention provides a new application of the ionizing radiation protection of Rosa roxburghii polysaccharide, which has not been reported in the prior art

Method used

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  • New application of polysaccharides from fructus rosae roxburghii for protection against ionizing radiation
  • New application of polysaccharides from fructus rosae roxburghii for protection against ionizing radiation
  • New application of polysaccharides from fructus rosae roxburghii for protection against ionizing radiation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] [Example 1] Cytotoxicity detection of Rosa roxburghii polysaccharides on V79 cells

[0018] Cell characteristics: Chinese hamster lung fibroblast V79, adherent growth, doubling time 12-14 hours.

[0019] 1. Culture conditions

[0020] Cells were cultured in DMEM medium (containing 10% FBS, 100 U / mL penicillin and 100 U / mL streptomycin), 37°C, 5% CO 2 Incubator, replace the culture medium every 2-3 days, the recommended cell culture density is 1-2×10 5 / mL.

[0021] 2. Drug preparation

[0022] Dissolve the Rosa roxburghii polysaccharide in DMEM culture solution, ultrasonically assist in dissolving, and prepare a stock solution with a storage concentration of 10 mg / ml for future use.

[0023] 3. Toxicity testing

[0024] Take V79 cells in the logarithmic growth phase (1×10 5 each / mL) were inoculated in a 96-well culture plate, 100 μL / hole, 100 μL of medicine in each well, so that the final concentration of the medicine was 2000, 1000, 500, 100, 50, 25, 12.5 μg / mL (...

Embodiment 2

[0025] [Embodiment two] Rosa roxburghii polysaccharide pair 60 C 0 Protective effect of loss of viability of V79 cells induced by γ-rays

[0026] 1. The ionizing radiation protection effect of different concentrations of drugs on V79 cells

[0027] Take V79 cells in the logarithmic growth phase (1×10 5 1 / mL) were inoculated in a 96-well culture plate, 100 μL / well, 100 μL of drug per well, so that the final drug concentration was 400, 200, 100, 50, 25, 12.5 μg / mL, 6 wells for each concentration, and a blank control group was set up Cell suspension, cell suspension of irradiation group. After 24 hours of drug action, 5Gy was irradiated. 24 hours after irradiation, 10 μL of CCK-8 solution was added to each well, and the culture was continued for 4 hours. The absorbance value of each well was measured at 490 nm in a multifunctional microplate reader. Compared with the irradiation group: #, P60 The loss of viability of V79 cells irradiated with 5Gy of COγ rays showed a dose-dep...

Embodiment 3

[0031] [Embodiment three] Rosa roxburghii polysaccharide pair 60 C 0 The effect of 8Gy γ-ray whole-body irradiation on the survival rate of Kunming mice

[0032] Animal grouping, dosing and irradiation:

[0033] After adapting to the environment, male Kunming mice were divided into normal group, irradiation group, positive drug group and drug group according to needs, with 10 mice in each group. The normal group and the irradiation group were given distilled water by intragastric administration, the drug group was given the corresponding drug by intragastric administration for 6 consecutive days, and the positive drug group was given "523" once by intragastric administration 24 hours before the irradiation, with a dose of 5 mg / kg, and the whole body was irradiated once after administration. , the dose was 8Gy, and the patients were continuously observed for 30 days after exposure. The results showed that the 30-day survival rate of mice given 100mg / kg Rosa roxburghii polysa...

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Abstract

The invention belongs to the technical field of medicine and provides a new application of polysaccharides from fructus rosae roxburghii for protection against ionizing radiation. Polysaccharides from fructus rosae roxburghii are prepared by a water extraction and alcohol precipitation method. Experiments on cells and animals for protection against ionizing radiation prove that the polysaccharides from fructus rosae roxburghii are nontoxic to V79 cells when the concentration is smaller than 1000 mu g / mL, have dosage-dependent inhibiting effect on the fact that 60Co gamma ray devitalizes the V79 cells when the concentration is 12.5-200 mu g / mL and can increase the survival rate of Kunming mice under the 8Gy irradiation of 60Co gamma ray in a dosage-dependent way in case of a prophylactic dose of 50 or 100mg / kg, thereby indicating that the polysaccharides from fructus rosae roxburghii have pharmaceutical activity for protection against ionizing radiation. Thus, the invention lays a solid foundation for developing a new oral medicament for protection against ionizing radiation.

Description

technical field [0001] The invention relates to the application of Rosa roxburghii polysaccharide, an effective part of traditional Chinese medicine, in ionizing radiation protection, and belongs to the technical field of medicine. Background technique [0002] Malignant tumor patients are a special group exposed to radiation. At present, radiation therapy is still an important means of treating tumors. According to reports, about 70% of malignant tumor patients in my country need radiation therapy. In addition, there are many opportunities for people to be exposed to ionizing radiation in the real environment, such as residents living in areas with high natural radiation, medical workers who are often exposed to radiation, space radiation received by astronauts, and nuclear leakage accidents in nuclear power plants. Long-term excessive ionizing radiation will cause a series of damage to the body, including oxidative stress damage, abnormal function of the hematopoietic syst...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/715A61P39/06
Inventor 徐萍杨春广张疆莉阎玺庆马素英
Owner XINXIANG MEDICAL UNIV
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