Novel application of flavonoid of rosa roxburghii tratt to ionizing radiation protection
A technology of ionizing radiation and Rosa roxburghii flavonoids, which is applied in the field of medicine, can solve problems that have not been reported in the prior art, and achieve the effects of broad medicinal prospects, high yield, and easy availability of raw materials
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Embodiment 1
[0017] [Example 1] Cytotoxicity detection of Rosa flavonoids on AHH-1 cells
[0018] Cell characteristics: Human lymphoblastoid cells AHH-1 grow in clusters in suspension with a doubling time of 16-19 hours.
[0019] Culture conditions: cells were cultured in RPM I1640 medium (containing 10% FBS, 100U / mL penicillin and 100U / mL streptomycin), 37°C, 5% CO 2 Incubator, replace the culture medium every 2-3 days, the recommended cell culture density is 1-2×10 5 / m L. Drug preparation: Dissolve roxathorn flavonoids in DMEM culture solution, sonicate to assist dissolution, and configure a stock solution with a storage concentration of 10 mg / ml for future use.
[0020] Toxicity test: AHH-1 cells in the logarithmic growth phase (1×10 5 each / mL) were inoculated in a 96-well culture plate, 100 μL / well, 100 μL of medicine per well, so that the final concentration was 240, 120, 60, 30, 15, 7.5, 3.75 μg / mL (referred to as drug group), 6 wells per concentration , and set up a blank contr...
Embodiment 2
[0021] [Embodiment 2] Prickly pear flavonoids to 60 C 0 Protective effect of loss of viability of AHH-1 cells induced by γ-rays
[0022] AHH-1 cells (1×10 5 cells / mL) were inoculated in a 96-well culture plate, 100 μL / well, 100 μL of drug per well, so that the final concentration was 60, 30, 15, 7.5, 3.75, 1.875 μg / mL, 6 wells per concentration, and a blank control group Suspension, cell suspension of irradiation group. After 24 hours of drug action, 4Gy was irradiated. 24 hours after irradiation, 10 μL of CCK-8 solution was added to each well, and the culture was continued for 4 hours. The absorbance value of each well was measured at 490 nm in a multifunctional microplate reader. Compared with the irradiation group: #, P60 The loss of viability of AHH-1 cells irradiated by CO γ-ray 4Gy showed a dose-dependent protective effect, and the optimal concentration for the protection of AHH-1 cells from ionizing radiation was 30 μg / mL. (see attached figure 2 )
Embodiment 3
[0023] [Embodiment three] Rosa roxburghii flavonoids 60 C 0 The effect of 8Gy γ-ray whole-body irradiation on the survival rate of Kunming mice
[0024] After adapting to the environment, the male Kunming mice were divided into normal group, irradiation group, positive drug group and medication group according to needs, with 10 mice in each group. The normal group and the irradiation group were given distilled water by intragastric administration, the drug group was given the corresponding drug by intragastric administration for 3 consecutive days, and the positive drug group was given "E523" once by intragastric administration 24 hours before the irradiation, with a dose of 5 mg / kg, and the whole body was irradiated once after administration. , the dose was 8Gy, and the patients were continuously observed for 30 days after exposure. The results show that the 30-day survival rate can reach 80% when 60 mg / kg of Rosa roxburghii is given to mice, and the animal experiment show...
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