Chromatographic separation material based on copolymerization on silica gel surface and preparation thereof

A technology of copolymerization reaction and chromatographic separation, which is applied in the field of silica gel substrate-bonded "non-polar/polar copolymerization stationary phase" and its preparation, can solve the problems of difficult regulation and fixed spatial position, etc., and achieve large bonding amount and improved Effect of selectivity and wide application of technology
CN101987293AActive Publication Date: 2011-03-23浙江华谱新创科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
浙江华谱新创科技有限公司
Publication Date
2011-03-23

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Abstract

The invention relates to a silica gel matrix separation material, wherein two or more silane reagents form nonpolar / polar copolymerization stationary phase through the copolymerization on silica gel surface. The structural formula of the nonpolar / polar copolymerization stationary phase is as follows: FORMULA, wherein Silica Gel is silica gel; NP stands for nonpolar group, which is one or more of C1 to C30 normal chain alkyl and phenyl; and P stands for polar group, which is one or more kinds of C1 to C12 normal chain alkyl whose tail end has the functional group such as chlorine atom, bromineatom, cyan, amido, benzenesulfonic acid group, sulfo group, carboxyl, quaternary ammonium group and alcohol group. The bonded phase of the separation material has novel structure, and simultaneously has nonpolar group and polar group, thereby being capable of providing hydrophobic force and polymorphic polar acting force, and greatly improving the selectivity of the inverse and efficient liquid chromatogram. The stationary phase prepared with the preparation method provided by the invention has the advantages of even and stable surface bonding group, large bonding quantity, and the like.
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Description

technical field

[0001] The invention relates to a chromatographic separation material, specifically a silica gel substrate-bonded "nonpolar / polar copolymerization stationary phase" based on silica gel surface copolymerization reaction and a preparation method thereof. Background technique

[0002] Reversed-phase high-performance liquid chromatography is the most widely used separation and analysis technique. Silica-based octadecyl-bonded stationary phase is the most commonly used separation material for reversed-phase chromatography. However, the octadecyl stationary phase can only provide a single hydrophobic force, and has poor retention and separation selectivity for polar compounds. Therefore, on the basis of conventional reversed-phase stationary phases, polar groups are introduced, and bonded stationary phases that can provide both hydrophobic and polar interaction forces are developed to supplement the polar selectivity in the reversed-phase chromatography separation...

Claims

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