Method for preparing spirulina protein isolate
A technology of protein isolate and spirulina, which is applied in the field of preparation of spirulina protein isolate, can solve the problems of protein content, digestibility and bioavailability gap, limitation of processing and comprehensive utilization of spirulina products, consumers' feeling of taking medicine, etc. Achieve the effect of good deodorization effect, small denaturation and high solubility
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Embodiment 1
[0028] The spirulina (algae mud) feed liquid is processed under the condition of 6500 rpm with a high-speed colloid mill, and the cells are broken, and then petroleum ether is added to the spirulina algae mud feed liquid of the cell crushing, and the petroleum ether and the feed liquid are in the ratio of 1: Add in a ratio of 5, stir or vibrate, extract the pigment and fishy smell substances, and the number of extractions is 3 times, remove the petroleum ether solvent layer containing the pigment and fishy smell substances, and crush the lower layer of spirulina cells in a vacuum degasser. The temperature is 0.09MPa for degassing, and after degassing, the decolorized and deodorized spirulina cell crushing feed liquid is obtained;
[0029] Add NaCl to the resulting decolorized and depigmented spirulina cell crushed material liquid to adjust to a dispersion with a NaCl concentration of 0.15 mol / L, so that the ratio of the spirulina cell crushed material to dilute brine is 1:10, a...
Embodiment 2
[0033] The spirulina (algae mud) feed liquid is treated with a high-speed colloid mill at a speed of 7000rpm, and the cells are broken, and then n-hexane is added to the broken spirulina algae feed liquid, and the ratio of n-hexane and feed liquid is 1: Add in a ratio of 5, stir or vibrate, extract the pigment and fishy smell substances, the extraction times are 3 times, remove the n-hexane solvent layer containing the pigment and fishy smell substances, and put the lower layer of spirulina cell crushed material in a vacuum degasser to vacuum The temperature is 0.08MPa for degassing, and after degassing, the decolorized and deodorized spirulina cell broken feed liquid is obtained;
[0034] Add Na2 SO 4 , adjusted to Na 2 SO 4 The concentration of the dispersion is 0.07mol / L, so that the ratio of spirulina cell crushing material to dilute brine is 1:15. Under the condition of temperature of 55°C, extract for 1.5 hours, cool to room temperature, and then centrifuge, the centri...
Embodiment 3
[0038] The spirulina (algae mud) feed liquid is processed under the condition of 7500 rpm with a high-speed colloid mill, and the cells are broken, and then gasoline is added to the spirulina algae mud feed liquid of the cell crushing, and the gasoline and the feed liquid are mixed according to the ratio of 1:5. Proportional addition, stirring or shaking, extraction of pigments and fishy substances, the number of extractions is 3 times, remove the gasoline solvent layer containing pigments and fishy substances, and crush the lower layer of spirulina cells in a vacuum degasser with a vacuum degree of 0.07 MPa is degassed, and after degassing, the decolorized and fishy spirulina cell crushing feed liquid is obtained, which is for subsequent use;
[0039] Add Na 2 HPO 4 , adjusted to Na 2 HPO 4 Concentration of 0.20mol / L dispersion liquid, so that the ratio of spirulina cell crushing material to dilute brine is 1:20, under the condition of temperature of 50 ℃, extract for 1 ho...
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