Method for preparing spirulina protein isolate

A technology of protein isolate and spirulina, which is applied in the field of preparation of spirulina protein isolate, can solve the problems of protein content, digestibility and bioavailability gap, limitation of processing and comprehensive utilization of spirulina products, consumers' feeling of taking medicine, etc. Achieve the effect of good deodorization effect, small denaturation and high solubility

Inactive Publication Date: 2012-08-15
丽江格林斯通食品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although spirulina is a high-quality complete protein nutrient source, its cell wall contains less cellulose, its protein digestibility can reach 75.0%, and its bioavailability can reach 68.0%. There are some limitations in the use of protein supplements: first, the variety of spirulina products is relatively single, and most of them appear in the form of tablets or pills, which can easily give consumers the feeling of taking medicine; second, spirulina...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The spirulina (algae mud) feed liquid is processed under the condition of 6500 rpm with a high-speed colloid mill, and the cells are broken, and then petroleum ether is added to the spirulina algae mud feed liquid of the cell crushing, and the petroleum ether and the feed liquid are in the ratio of 1: Add in a ratio of 5, stir or vibrate, extract the pigment and fishy smell substances, and the number of extractions is 3 times, remove the petroleum ether solvent layer containing the pigment and fishy smell substances, and crush the lower layer of spirulina cells in a vacuum degasser. The temperature is 0.09MPa for degassing, and after degassing, the decolorized and deodorized spirulina cell crushing feed liquid is obtained;

[0029] Add NaCl to the resulting decolorized and depigmented spirulina cell crushed material liquid to adjust to a dispersion with a NaCl concentration of 0.15 mol / L, so that the ratio of the spirulina cell crushed material to dilute brine is 1:10, a...

Embodiment 2

[0033] The spirulina (algae mud) feed liquid is treated with a high-speed colloid mill at a speed of 7000rpm, and the cells are broken, and then n-hexane is added to the broken spirulina algae feed liquid, and the ratio of n-hexane and feed liquid is 1: Add in a ratio of 5, stir or vibrate, extract the pigment and fishy smell substances, the extraction times are 3 times, remove the n-hexane solvent layer containing the pigment and fishy smell substances, and put the lower layer of spirulina cell crushed material in a vacuum degasser to vacuum The temperature is 0.08MPa for degassing, and after degassing, the decolorized and deodorized spirulina cell broken feed liquid is obtained;

[0034] Add Na2 SO 4 , adjusted to Na 2 SO 4 The concentration of the dispersion is 0.07mol / L, so that the ratio of spirulina cell crushing material to dilute brine is 1:15. Under the condition of temperature of 55°C, extract for 1.5 hours, cool to room temperature, and then centrifuge, the centri...

Embodiment 3

[0038] The spirulina (algae mud) feed liquid is processed under the condition of 7500 rpm with a high-speed colloid mill, and the cells are broken, and then gasoline is added to the spirulina algae mud feed liquid of the cell crushing, and the gasoline and the feed liquid are mixed according to the ratio of 1:5. Proportional addition, stirring or shaking, extraction of pigments and fishy substances, the number of extractions is 3 times, remove the gasoline solvent layer containing pigments and fishy substances, and crush the lower layer of spirulina cells in a vacuum degasser with a vacuum degree of 0.07 MPa is degassed, and after degassing, the decolorized and fishy spirulina cell crushing feed liquid is obtained, which is for subsequent use;

[0039] Add Na 2 HPO 4 , adjusted to Na 2 HPO 4 Concentration of 0.20mol / L dispersion liquid, so that the ratio of spirulina cell crushing material to dilute brine is 1:20, under the condition of temperature of 50 ℃, extract for 1 ho...

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Abstract

The invention relates to a method for preparing spirulina protein isolate, and belongs to the technical field of food science and engineering. The method is characterized by comprising the following steps of: (1) preparing a spirulina cell disrupted, decolorized, deodorized and degreased material; (2) extracting protein by using dilute solution of salt and centrifuging; (3) adjusting the pH untilthe solution is acidic in a gradient and sectional way, performing acid precipitation to obtain the protein, and adjusting the pH until the solution is neutral; and (4) performing ultra-high temperature short-time sterilization and spray drying. The spirulina protein isolate produced by the method has the advantages of light color, light fishy odor, high protein content, high dissolubility and easiness of digestion, and can be used as a high-quality full-value protein nutritional supplement or additive.

Description

Technical field: [0001] The invention relates to a method for preparing isolated protein from spirulina, which belongs to the technical field of food science and engineering. Background technique: [0002] Spirulina (Spirulina), also known as cyanobacteria, belongs to Cyanophyta, Cyanophyceae, Oscillatoriaceae, Oscillatoriaceae, Spirulina or Arthrospira plants, is a multicellular filamentous cyanobacteria, composed of short cylindrical The cells are composed of filamentous spirals. Spirulina is native to Africa and North America. It is a low-level aquatic plant. There are currently 36 known species of Spirulina, which are widely distributed in oceans, lakes, and hot springs in tropical, subtropical, and warm temperate zones, especially saline lakes. . At present, the industrially produced spirulina at home and abroad mainly refers to the larger Spirulina platensis (Spirulina platensis) and Spirulina maxima (Spirulina maxima). [0003] The biochemical composition of spirul...

Claims

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Application Information

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IPC IPC(8): C07K14/405C07K1/30C07K1/14
Inventor 吴光旭申艮宝栗永茂刘素霞
Owner 丽江格林斯通食品有限公司
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