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Gene recombination human collagen fusion peptide segment, preparation method and application thereof

A human collagen and gene recombination technology, applied in the biological field, can solve the problems of biosafety and biocompatibility, and the human body is not identical.

Active Publication Date: 2011-04-20
SHAANXI HUIKANG BIO TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] The collagen currently sold in the market is a collagen mixture peptide segment extracted from animal skin, tendon and other tissues by acid-base hydrolysis. The amino acid sequence of these peptide segments is not exactly the same as that of the human body, so its biosafety and biocompatibility are in the Research and development of medical materials is limited

Method used

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  • Gene recombination human collagen fusion peptide segment, preparation method and application thereof
  • Gene recombination human collagen fusion peptide segment, preparation method and application thereof
  • Gene recombination human collagen fusion peptide segment, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0139] The total length of the gene recombinant human collagen fusion peptide is 839 amino acids, and its nitrogen terminal is the 908-1137 peptide of type III human collagen, a total of 229 amino acids, and its carbon terminal is the 497-1104 peptide of type I human collagen A total of 608 amino acids, the two peptides are linked by glutamic acid and phenylalanine; the amino acid sequence of the recombinant human collagen fusion peptide is as follows:

[0140] AGNTGAPGSP GVSGPKGDAG QPGEKGSPGA QGPPGAPGPL GIAGITGARG

[0141] LAGPPGMPGP RGSPGPQGVK GESGKPGANG LSGERGPPGP QGLPGLAGTA 100

[0142] GEPGRDGNPG SDGLPGRDGS PGGKGDRGEN GSPGAPGAPG HPGPPPGVGP

[0143] AGKSGDRGES GPAGPAGAPG PAGSRGAPGP QGPRGDKGET GERGAAGIKG 200

[0144] HRGFPGNPGA PGSPGPAGQQ GAIGSPGPAE FGADGVAGPK GPAGERGSPG

[0145] PAGPKGSPGE AGRPGEAGLP GAKGLTGSPG SPPGDGKTGP PGPAGQDGRP 300

[0146] GPPGPPGARG QAGVMGFPGP KGAAGEPGKA GERGVPGPPG AVGPAGKDGE 350

[0147] AGAQGPPGPA GPAGERGEQG PAGSPGFQGL PGPAGPPGEA GKPGEQGVPG 4...

Embodiment 2

[0377] The recombinant human collagen fusion peptide prepared in Example 1 of the present invention is used to prepare cosmetics, which are made of the following raw materials in mass ratio:

[0378] Recombinant Human Collagen Fusion Peptide 10g

[0379] Hyaluronic Acid 0.5g

[0380] Glycerin 25g

[0381] Deionized water 1000g.

[0382] Its preparation method is as follows:

[0383] Take dry powdered gene recombinant human collagen fusion peptide, hyaluronic acid, glycerin, dissolve in deionized water, adjust the pH value to 6.5-7.5, and filter it with a 0.22um filter membrane in a sterile environment to obtain colorless and transparent Liquid, sealed in 5ml glass bottle or plastic bottle by aseptic method, stored in refrigerator at 4°C.

Embodiment 3

[0385] The recombinant human collagen fusion peptide prepared in Example 1 of the present invention is used to prepare cosmetics, which are made of the following raw materials in mass ratio:

[0386] Recombinant Human Collagen Fusion Peptide 8g

[0387] Hyaluronic Acid 0.3g

[0388] Glycerin 20g

[0389] Deionized water 1000g.

[0390] Its preparation method is identical with embodiment 2.

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Abstract

The invention relates to a gene recombination human collagen fusion peptide segment. The overall length of the peptide segment comprises 839 amino acids, the nitrogen end of the peptide segment comprises a type III human collagen peptide section of 908-1137, which totally contains 229 amino acids, and the carbon end of the peptide segment comprises a type I human collagen peptide segment of 497-1104, which total contains 608 amino acids, wherein the two peptide segments are connected by using glutamic acid and phenylalanine. The preparation method of the peptide segment comprises the following steps of: constructing cDNAs of clone type I (COL 1) and type III (COL 3) human collagen genes and a Pichia pastoris recombinant expression vector pPOC9K-COL3a1-COL1a1; constructing Pichia pastoris genetic engineering bacteria SMD1168-COL3a10COL1a1 of the gene recombination human collagen fusion peptide segment; preparing the gene recombination human collagen fusion peptide segment, and the like. Proved by tests, the gene recombination human collagen fusion peptide segment prepared by adopting the method can be used as a material for preparing cosmetics.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a fusion peptide segment of gene recombinant human collagen. Background technique [0002] Collagen is a family of proteins widely distributed in animal connective tissues, and it is also the most abundant protein in the body, accounting for about 25-33% of the total protein. and damage repair. As a natural biological resource, collagen has biocompatibility and biodegradability unmatched by synthetic polymer materials. It can be widely used in medicine, cosmetics and other industries. It can maintain the elasticity of blood vessel walls, prevent embolism, improve cartilage and Lubrication of ligaments, alleviation of joint stiffness, etc., its role in treating dermal damage or defects, cosmetic surgery, improving sleep, and enhancing immunity has been agreed upon. At the same time, they contain a large number of polar groups and natural moisturizing factors such as glyci...

Claims

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Application Information

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IPC IPC(8): C07K19/00C07K1/36C07K1/34C07K1/18C12N15/62C12N15/81C12N1/19C12P21/02A61K8/65
Inventor 张凤龙侯曾淼赵金礼
Owner SHAANXI HUIKANG BIO TECH CO LTD
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