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Method for preparing gardenia blue pigment

A gardenia blue pigment and gardenia fruit technology, applied in chemical instruments and methods, azo dyes, organic dyes, etc., can solve the problems of high cost, unsuitable production process for large-scale industrial production, low color price and the like

Active Publication Date: 2011-04-20
GUILIN NATURAL INGREDIENTS CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Generally speaking, most domestically produced gardenia blue pigments are low in color and high in cost, and the production process is not suitable for large-scale industrial production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] 1) Get the gardenia fruit and pulverize it, extract it with 50% ethanol (the weight unit of the gardenia fruit powder is kilogram, and the volume unit of ethanol is liter, the same below) that is equivalent to 5 times the weight of the gardenia fruit powder, and extract for 2 hours, Filter and concentrate to obtain a concentrated solution;

[0018] 2) Put the concentrated solution on an AB-8 macroporous resin column, elute with ethanol with a volume concentration of 35%, and obtain the crude geniposide;

[0019] 3) The crude product of geniposide is separated with an alumina adsorption column to obtain geniposide, and 1%, 8%, and 8% of the weight of gardenia fruit powder are added to geniposide, sodium chloride, cellulase, and glutenase are respectively added. Amino acid was hydrolyzed for 10 hours at 50°C to obtain a reaction solution, and the conversion rate of geniposide measured by HPLC was 88%;

[0020] 4) The reaction solution was dried to obtain a solid powder o...

Embodiment 2

[0028] 1) Grind the fruit of Gardenia jasminoides, extract twice with ethanol having a volume equivalent to 8 times the weight of gardenia fruit powder, and a volume concentration of 50%, for 1 hour each time, filter, combine the filtrates, and concentrate to obtain a concentrate;

[0029] 2) Put the concentrated solution on a D101 type macroporous resin column, elute with ethanol with a volume concentration of 25%, and obtain the crude product of geniposide;

[0030] 3) The crude product of geniposide is separated by an alumina adsorption column to obtain geniposide, and 1.5%, 5%, and 10% of the weight of genipo fruit powder are respectively added to geniposide, sodium chloride, cellulase and glycine , hydrolyzed at 55°C for 14 hours to obtain a reaction solution, and the conversion rate of geniposide measured by HPLC was 94%;

[0031] 4) The reaction solution was dried to obtain a gardenia blue pigment solid powder, which had a bright and natural color and a color value of 9...

Embodiment 3

[0033] 1) Grind the fruit of Gardenia jasminoides, extract twice with ethanol equivalent to 7 times the weight of gardenia fruit powder, and have a volume concentration of 60%, for 2 hours each time, filter, combine the filtrates, and concentrate to obtain a concentrated solution;

[0034] 2) Put the concentrated solution on a D101 type macroporous resin column, elute with ethanol with a volume concentration of 30%, and obtain the crude product of geniposide;

[0035] 3) The crude product of geniposide is separated by an alumina adsorption column to obtain geniposide, and 0.5%, 8%, and 5% of the weight of genipo fruit powder are respectively added to geniposide, sodium sulfate, cellulase and glutamine Na2SO4 was hydrolyzed for 8 hours at 60°C to obtain a reaction solution, and the conversion rate of geniposide measured by HPLC was 86%;

[0036] 4) The reaction solution was dried to obtain a gardenia blue pigment solid powder, which had a bright and natural color and a color va...

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Abstract

The invention relates to a method for preparing a gardenia blue pigment. The method comprises the following steps of: 1) extracting gardenia fruit powder with 50 to 60 volume percent ethanol in an amount which is 5 to 8 times the volume of the gardenia fruit powder, filtering, and concentrating to obtain concentrated solution; 2) loading the concentrated solution to a macroporous resin column, and eluting with 25 to 35 volume percent ethanol to obtain a gardenoside crude product; 3) separating the gardenoside crude product with an alumina adsorption column to obtain gardenoside, adding cellulase, a hydrolyst and amino acid into the gardenoside for hydrolysis to obtain reactive fluid, wherein the cellulase and the amino acid are added in an amount which is 5 to 10 percent based on the weight of the gardenia fruit powder; the active concentration of the cellulase is 1 mg / ml; the hydrolyst is added in an amount which is 0.5 to 1.5 percent based on the weight of the gardenia fruit powder; the hydrolysis temperature is between 50 and 60 DEG C and the hydrolysis time is 8 to 16 hours; and 4) drying the reactive fluid to obtain the gardenia blue pigment. The method has a short production cycle, and the obtained gardenia blue pigment has bright colour and high colourity.

Description

technical field [0001] The invention relates to a method for preparing natural pigment, in particular to a method for extracting and preparing gardenia blue pigment from gardenia. Background technique [0002] Gardenia is the fruit of Gardenia jasminoides Ellis (Gardenia jasminoides Ellis), a plant of Rubiaceae (Rubiaceae), oblong or oval, dark red or yellow-red in color. Gardenia belongs to the first batch of food and medicine dual-purpose resources promulgated by the Ministry of Health. It is a traditional Chinese medicine, which has the functions of protecting the liver, promoting gallbladder, lowering blood pressure, stopping bleeding, clearing away heat, benefiting cold, and stopping diarrhea. "Compendium of Materia Medica" has detailed records: Gardenia has the functions of promoting gallbladder and protecting liver, clearing heat and detoxifying, lowering blood pressure, antibacterial and anti-inflammatory. In traditional Chinese medicine, it is often used to treat j...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P17/06C09B61/00
Inventor 孙步祥汤凌志陈振鹏吴柏峰易永基陆玮
Owner GUILIN NATURAL INGREDIENTS CORP
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