Secondary enzymolysis method for preparing heme iron for feeds
A technology of secondary enzymatic hydrolysis and heme iron, applied in animal feed, animal feed, application, etc., can solve the problems of low iron content, low absorption rate, and low heme purity, and achieve high heme iron content. , the effect of high absorption rate
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[0025] Take fresh pig blood, add sodium citrate with a mass fraction of 10% to make the content of sodium citrate 4.5‰; use a tube centrifuge to separate plasma and blood cells, the centrifugation conditions are: 3500~7000r / min speed centrifugation 15min. Take the blood cell solution, add 2 times the volume of water to the blood cell solution, start stirring for 1-3 hours, and break the blood cell; after breaking the wall, raise the temperature of the blood cell solution to 55°C, add 5‰ animal proteolytic enzyme, and enzymatically hydrolyze for 12 hours , adjust the pH to 5.0 with 6mol / L hydrochloric acid, filter the enzymatic solution through a filter press, collect the filter cake on the filter cloth, add 5 times of water to dissolve, heat up to 55°C, add 2.5‰ of animal proteolytic enzyme, After 10 hours of enzymatic hydrolysis, adjust the pH to 5.0 with 6 mol / L hydrochloric acid, filter the enzymolyzed solution through a filter press, collect the filter cake, and dry it in ...
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