Method for preparing spherical barium carbonate powder
A barium carbonate and spherical technology is applied in the field of preparation of spherical barium carbonate powder to achieve the effects of improving yield, enhancing enzymatic action and facilitating growth and reproduction
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Embodiment 1
[0034] A preparation method utilizing microorganisms to induce barium carbonate precipitation, comprising: glucose 10-60g, peptone 5-50g, sodium chloride 1-10g, urea 10-60g, Ba(NO 3 ) 2 1-10g and 1000g of distilled water are mixed to prepare a liquid medium, adjust the pH value to 6-9, and sterilize. Urea and calcium nitrate were added to the above-mentioned medium through the method of microporous membrane filter sterilization. Inoculate the liquid strain of Bacillus pasteurii (Bacillus pasteurii) into the liquid culture medium, shake and culture for 48 hours at 25-40°C, 100-200r / min, rest for 12-36 hours, filter with distilled water and absolute ethanol Wash and dry at 45°C for 24 hours to obtain spherical barium carbonate powder.
Embodiment 2
[0036] A preparation method utilizing microorganisms to induce barium carbonate precipitation, comprising: glucose 10-60g, peptone 5-50g, sodium chloride 1-10g, urea 10-60g, Ba(NO 3 ) 21-10g, Tween 800.1%-0.3% (V / V), and distilled water 1000g are mixed to form a liquid medium, the pH value is adjusted to 6-9, and sterilized. Urea and calcium nitrate were added to the above-mentioned medium through the method of microporous membrane filter sterilization. Inoculate the liquid strain of Bacillus pasteurii (Bacillus pasteurii) into the liquid culture medium, shake and cultivate for 48 hours at 25-40°C and 100-200r / min, rest for 12-36 hours, filter with suction, and use distilled water and anhydrous Wash with ethanol and dry at 45°C for 24 hours to obtain spherical barium carbonate powder.
Embodiment 3
[0038] A preparation method utilizing microorganisms to induce barium carbonate precipitation, comprising: glucose 10-60g, peptone 5-50g, sodium chloride 1-10g, urea 10-60g, Ba(NO 3 ) 2 1-10g, nickel chloride 50-250μmol / L, Tween 800.1%-0.3% (V / V), and distilled water 1000g are mixed to form a liquid medium, the pH value is adjusted to 6-9, and sterilized. Nickel chloride, urea and calcium nitrate were added to the above-mentioned culture medium by filtering and sterilizing through a microporous membrane. Inoculate the liquid strain of Bacillus pasteurii (Bacillus pasteurii) into the liquid culture medium, shake and culture for 48 hours at 25-40°C, 100-200r / min, rest for 12-36 hours, filter with distilled water and absolute ethanol Wash and dry at 45°C for 24 hours to obtain spherical barium carbonate powder.
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