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Method for producing quadruple inactivated vaccine for newcastle disease, infectious bronchitis, avian influenza (H9 subtype) and infectious bursal disease

A technology for bronchitis and bursal disease, applied in antiviral agents, pharmaceutical formulas, virus antigen components, etc., can solve the problems of uncontrollable epidemic development, single traditional vaccine, and affecting production performance, so as to reduce the occurrence of epidemics and spread, rapid antibody production, and long protection period

Active Publication Date: 2012-11-21
YEBIO BIOENG OF QINGDAO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] There are many varieties of commercial products on the market at home and abroad, but they are all single traditional vaccines. Multiple use of vaccines, especially inactivated vaccines, not only increases the burden of manpower and material resources on the chicken farm, but also causes the injection stress of catching chickens many times. Can affect performance and increase susceptibility of flocks to disease
Coupled with the continuous mutation of virus strains in recent years, although a variety of vaccines have been introduced, the development of the epidemic is still uncontrollable.

Method used

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  • Method for producing quadruple inactivated vaccine for newcastle disease, infectious bronchitis, avian influenza (H9 subtype) and infectious bursal disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0073] (1) Antigen preparation and inspection of semi-finished products:

[0074] 1. Preparation of Chicken Newcastle Disease Virus Liquid

[0075] (1) Inoculation Take the virus seeds for production and make appropriate dilution with sterilized physiological saline (such as 10 -4 or 10 -5 ), inoculate 6528 10-11-day-old susceptible chicken embryos, 0.1ml per embryo, seal the pinhole after inoculation, and continue incubation at 36-37°C without turning the embryos.

[0076] (2) Incubation and observation After chicken embryos were inoculated, the embryos were photographed once a day, and the chicken embryos that died 60 hours ago were discarded. Thereafter, the embryos were photographed once every 4-6 hours, and the dead embryos were taken out at any time until 96 hours, regardless of whether they were dead or not, all were taken out, the air chamber was upward, and they were cooled at 2-8°C for 12-24 hours.

[0077] (3) Harvesting Take out the cooled chicken embryos, and h...

Embodiment 2

[0121] Product testing

[0122] 1. traits

[0123] Appearance Milky white emulsion.

[0124] Dosage form water-in-oil type. Take a clean straw, suck a small amount of vaccine and drop it into cold water, except for the first drop, it should not spread.

[0125] Stability Take 10ml of the vaccine and put it into a centrifuge tube, centrifuge at 3000r / min for 15 minutes, the water precipitated at the bottom of the tube should be ≤0.5ml.

[0126] Viscosity Use a 1ml straw (the inner diameter of the lower mouth is 1.2mm, and the inner diameter of the upper mouth is 2.7mm) to draw 1ml of vaccine at about 25°C, let it flow out vertically and naturally, and record the time required for the outflow of 0.4ml, which should be within 6 seconds.

[0127] 2. Sterility test According to the current "Chinese Veterinary Pharmacopoeia", it should grow aseptically.

[0128] 3. Safety inspection Use 10 SPF chickens aged 7 to 14 days, inject 1ml of vaccine subcutaneously in each muscle or nec...

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Abstract

The invention relates to a method for producing a quadruple inactivated vaccine for newcastle disease, infectious bronchitis, avian influenza (H9 subtype) and infectious bursal disease. In the method, the inactivated vaccine is prepared by adopting a newcastle disease virus La Sota strain, an infectious bronchitis virus M41 strain, an avian influenza virus (H9 subtype) YBF003 strain, and an Escherichia coli genetic engineering bacteria E. coil BL21 / pET28a-VP2 strain which expresses an infectious bursal disease virus VP2 protein serving as production bacteria toxic species, a super concentration process and a high-quality adjuvant. After immunizing animals, antibodies are produced rapidly; the potency is high; the protection period is long; and the outbreak and the spreading of epidemic diseases are reduced.

Description

technical field [0001] The invention relates to a method for preparing an oil-emulsion vaccine for preventing Newcastle disease, infectious bronchitis, avian influenza (subtype H9) and infectious bursal disease in chickens, and belongs to the field of veterinary biological products. Background technique [0002] Newcastle disease (ND) has a high morbidity and fatality rate, and it occurs frequently all over the world. Once it breaks out, it will bring a devastating blow to the poultry industry. It is a major infectious disease that endangers the poultry industry. OIE lists it as a Category A disease. (Yin Zhen, Liu Jinghua, 1997. Animal Virology. Beijing. Science Press) [0003] Chicken infectious bronchitis (Infectious Bronchitis, IB) infection can lead to slow growth of chicks and broilers, decreased egg production and decreased egg quality of laying hens. The OIE lists it as a Category B disease. (Yin Zhen, Liu Jinghua, 1997. Animal Virology. Beijing. Science Press) ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/295A61P31/14A61P31/16A61K39/12A61K39/145A61K39/17A61K31/215
Inventor 李陆梅宫晓李昌友左青山李晓林孙健
Owner YEBIO BIOENG OF QINGDAO
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