Hydrogel dressing containing recombinant human epidermal growth factor and preparation method and application thereof

An epidermal growth factor and hydrogel technology, which is applied in the field of biomedical engineering materials to achieve good therapeutic effects, mild preparation conditions, and maintain activity.

Inactive Publication Date: 2011-06-01
SUN YAT SEN MEMORIAL HOSPITAL SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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  • Hydrogel dressing containing recombinant human epidermal growth factor and preparation method and application thereof
  • Hydrogel dressing containing recombinant human epidermal growth factor and preparation method and application thereof
  • Hydrogel dressing containing recombinant human epidermal growth factor and preparation method and application thereof

Examples

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Effect test

Embodiment 1

[0038] The preparation method of the hydrogel dressing containing recombinant human epidermal growth factor comprises the following steps:

[0039] (1) At 40°C, polyvinyl alcohol (PVA) (Xilong Chemical Factory, Shantou City, Guangdong Province, molecular weight 12-150,000) was prepared into a 5.0% aqueous solution, and sodium alginate (Dongsheng Chemical Reagent Factory, Wenzhou City, Zhejiang Province, Medium viscosity) is mixed with 2.0% aqueous solution, then polyvinyl alcohol and sodium alginate aqueous solution are mixed by 4: 1 (mass ratio) and are made into the polyvinyl alcohol / sodium alginate mixed solution of 89.95g, stir well, then add sodium benzoate (SB) 0.05g, 1mg rh-EGF (purchased from CYTOLAB / PEPROTECH ASIA), 10g glycerol, mixed and dissolved to obtain mixed solution A;

[0040] (2) At 40°C, prepare sodium borate and calcium chloride into 1% solutions respectively, and mix them at a volume ratio of 1:1 to obtain a cross-linking agent. Take 4.0 mL of the cross-l...

Embodiment 2

[0045] The preparation method of the hydrogel dressing containing recombinant human epidermal growth factor comprises the following steps:

[0046] (1) At 10°C, take polyvinyl alcohol (PVA) (Xilong Chemical Factory, Shantou City, Guangdong Province, molecular weight 120,000 to 150,000) to prepare a 2.0% aqueous solution, and get sodium alginate (Dongsheng Chemical Reagent Factory, Wenzhou City, Zhejiang Province, Medium viscosity) is mixed with 0.5% aqueous solution, then polyvinyl alcohol and sodium alginate aqueous solution are mixed by 5: 1 (mass ratio) and are made into the polyvinyl alcohol / sodium alginate mixed solution of 89.95g, stir well, then add sodium benzoate 0.03g, 1mg rh-EGF (purchased from CYTOLAB / PEPROTECH ASIA), 2.0g glycerol, mixed and dissolved to obtain mixed solution A;

[0047] (2) At 10°C, prepare sodium borate and calcium chloride into 2% solutions respectively, and mix them at a volume ratio of 1:1 to obtain a cross-linking agent. Take 2.0 mL of the c...

Embodiment 3

[0051] The preparation method of the hydrogel dressing containing recombinant human epidermal growth factor comprises the following steps:

[0052] (1) At 25°C, take polyvinyl alcohol (PVA) to prepare a 3.0% aqueous solution, take sodium alginate to prepare a 1.0% aqueous solution, and then mix polyvinyl alcohol and sodium alginate aqueous solution at 2:1 (mass ratio) Prepare 89.95g of polyvinyl alcohol / sodium alginate mixed solution, stir evenly, then add 0.04g of sodium benzoate, 1mgrh-EGF (purchased from CYTOLAB / PEPROTECH ASIA), 8.0g of glycerin, mix and dissolve to obtain mixed solution A;

[0053] (2) At 25°C, prepare sodium borate and calcium chloride into 1.5% solutions respectively, and mix them at a volume ratio of 1:1 to obtain a cross-linking agent. Take 8.0 mL of cross-linking agent and slowly drop them into 100 mL of mixed solution A. , stir evenly, stand still to remove foam, and obtain the hydrogel dressing containing recombinant human epidermal growth factor. ...

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Abstract

The invention discloses a hydrogel dressing containing a recombinant human epidermal growth factor. The hydrogel dressing consists of 100 volume parts of mixed solution A and 2 to 8 volume parts of cross-linking agent; the mixed solution A is prepared by mixing 2.0 to 5.0 mass percent of polyvinyl alcohol aqueous solution and 0.5 to 2.0 mass percent of sodium alginate aqueous solution according to the mass ratio of (2-5): 1, and adding 0 to 0.1 mass percent of sodium benzoate, 5 to 20ug/g of recombinant human epidermal growth factor and 2.0 to 10 mass percent of glycerol; and the gel dressing is prepared by taking the 2 to 8 volume parts of cross-linking agent prepared by mixing sodium borate solution and calcium chloride solution, dripping the cross-linking agent into the 100 volume parts of mixed solution A, standing and defoaming to obtain the gel dressing. The dressing has good biocompatibility and mild and simple preparation condition, can be formed at room temperature, can effectively keep the activity of the recombinant epidermal growth factor and has controlled release effect on the recombinant epidermal growth factor. The dressing has good effect of treating the wound of rats suffering from diabetes mellitus, and can accelerate healing of the wound.

Description

technical field [0001] The invention belongs to the field of biomedical engineering materials, and in particular relates to a hydrogel dressing containing recombinant human epidermal growth factor (rh-EGF) and a preparation method thereof, and its application as a medicine for treating diabetic foot chronic ulcer. Background technique [0002] Diabetic foot chronic ulcer seriously affects the life safety and quality of life of diabetic patients. It has a high incidence rate, great harm, and expensive treatment costs. Once a foot ulcer occurs, it is very difficult to treat. Studies have shown that decreased levels of growth factors are an important reason for delayed wound healing in diabetes. Since recombinant growth factors can accelerate wound healing, local application of exogenous growth factors is one of the commonly used clinical methods to accelerate the healing of diabetic foot ulcers. However, due to the short half-life of growth factors, they are easily degraded ...

Claims

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Application Information

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IPC IPC(8): A61L15/28A61L15/24A61L15/44A61K9/70A61K38/18A61P3/10A61P17/02
Inventor 杨川严励王建平张黎明黄杰伟马栋劳国娟
Owner SUN YAT SEN MEMORIAL HOSPITAL SUN YAT SEN UNIV
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