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Extraction and purification method of phlorizin in litchi peels

A technology of litchi peel and purification method, which is applied in chemical instruments and methods, organic chemistry, preparation of sugar derivatives, etc., can solve the problems of discovering phlorizin, etc., achieve the effects of wide sources, huge economic benefits, and promotion of development

Inactive Publication Date: 2011-06-08
GUANGZHOU TIANWEN JIAOYAN COSMETICS
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  • Abstract
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Problems solved by technology

However, before the study of this topic, there was no literature report on the discovery of phlorizin from litchi at home and abroad.

Method used

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  • Extraction and purification method of phlorizin in litchi peels
  • Extraction and purification method of phlorizin in litchi peels
  • Extraction and purification method of phlorizin in litchi peels

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Embodiment Construction

[0024] The present invention will be further described below in conjunction with specific examples.

[0025] 1. Prepare the main instruments, reagents and materials

[0026] Agilent 1100 high performance liquid chromatography (photodiode array detector), Ultimate C18 column (250×4.6mm, 5μm), Dikma C18 solid phase extraction column (500 mg, 3mL).

[0027] API 4000 QTrap Liquid Chromatography - Triple Quadrupole / Linear Ion Mass Spectrometer (Applied Biosystems, USA), Automatic Data Correlation - Enhanced Product Ion Scanning, Locked at 435.4 m / z, Collision Energy -15V, -30V, - 45V three superposition, scanning range 50-500 m / z.

[0028] KQ5200DA CNC ultrasonic cleaner, power 100W.

[0029] HGC-12 Nitrogen Blowing Apparatus (Tianjin Hengao Company), temperature 50°C.

[0030] D101 macroporous resin (Tianjin Nankai Hecheng Company).

[0031] The standard phlorizin was purchased from Sigma, USA, with a purity of ≥99.0%.

[0032] Methanol used as mobile phase was chromatographi...

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Abstract

The invention discloses an extraction and purification method of phlorizin in litchi peels, belonging to the field of cosmetic raw material development in natural plants. The extraction and purification method comprises the steps of smashing, extracting crude extract liquid, enriching litchi rind crude extract liquid, carrying out solid-phase extraction and purifying. Radically found in the litchi by the extraction and purification method, the phlorizin is purified out from the litchi peels and then is applied to cosmetic to serve as a natural raw material with skin whitening and anti-aging activities. The litchi peels has wide sources in Lingnan area of China, and wastes are turned into valuable things.

Description

technical field [0001] The invention relates to a method for extracting and purifying phloridzin, in particular to a method for extracting and purifying phloridzin from litchi peel, and belongs to the field of cosmetic raw material development in natural plants. Background technique [0002] Phloretin and its glucoside phloridzin belong to dihydrochalcones in flavonoids, and are a new type of skin whitening agent and antioxidant newly researched and developed at home and abroad. Dihydrochalcone is rarely distributed in nature, and phloretin and phloridzin exist in the rhizomes and root barks of some plants. According to literature reports, they are more abundant in apples, and most of them exist in the form of phloridzin [1-2] . litchi( Litchi chinensis Sonn. ) is a characteristic fruit abound in Lingnan area of ​​China. The research on litchi peel preliminarily deduces that it contains flavonoids [3] . However, before the research of this subject, there was no literatur...

Claims

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Application Information

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IPC IPC(8): C07H15/203C07H1/08
Inventor 赖维
Owner GUANGZHOU TIANWEN JIAOYAN COSMETICS
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