Extraction and purification method of phlorizin in litchi peels
A technology of litchi peel and purification method, which is applied in chemical instruments and methods, organic chemistry, preparation of sugar derivatives, etc., can solve the problems of discovering phlorizin, etc., achieve the effects of wide sources, huge economic benefits, and promotion of development
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[0024] The present invention will be further described below in conjunction with specific embodiments.
[0025] 1. Prepare main instruments, reagents and materials
[0026] Agilent 1100 high performance liquid chromatograph (photodiode array detector), Ultimate C18 column (250 × 4.6 mm, 5 μm), Dikma C18 solid phase extraction cartridge (500 mg, 3 mL).
[0027] API 4000 QTrap liquid chromatography-triple quadrupole / linear ion mass spectrometer (Applied Biosystems, USA), automatic data correlation-enhanced product ion scan, locked at 435.4 m / z, collision energy -15V, -30V, - 45V is superimposed on the three, and the scanning range is 50-500 m / z.
[0028] KQ5200DA CNC ultrasonic cleaner, power 100W.
[0029] HGC-12 nitrogen blowing instrument (Tianjin Hengao Company), the temperature was 50 ℃.
[0030] D101 macroporous resin (Tianjin Nankai Hecheng Company).
[0031] The standard substance of phloridzin was purchased from Sigma Company in the United States, and the purity was...
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