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Method for cloning rice complex trait associated gene

A technology related to rice, which is applied in the field of cloning genes related to rice complex traits, to achieve the effect of reducing costs, speeding up cloning, and heavy workload

Inactive Publication Date: 2011-06-15
SHANGHAI AGROBIOLOGICAL GENE CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] However, judging from the current research report, its polymorphism is only the basis for further sequencing verification, and then using the sequence to analyze the polymorphism of this site is an extension of the single nucleotide polymorphism technology

Method used

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  • Method for cloning rice complex trait associated gene
  • Method for cloning rice complex trait associated gene
  • Method for cloning rice complex trait associated gene

Examples

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Embodiment 1

[0028] Using the method of the present invention to clone genes associated with rice drought resistance

[0029] The drought resistance of rice is a typical complex trait, which is controlled by multiple genes and cooperates with the environment. More than 200 loci controlling the drought resistance of rice have been discovered through QTL mapping. 150 varieties of drought-resistant varieties at home and abroad were collected, and the drought-resistant traits of these varieties were identified on the artificial drought-resistant identification facility and related genes were cloned.

[0030] 1. Field experiment and trait investigation of rice varieties

[0031] Phenotypic tests were performed on field gradient moisture identification islands. Seeds of all tested varieties (150 varieties) were soaked to accelerate germination to dewy white, then sowed and raised, and transplanted according to normal field management methods, and planted in a single row. A random block design wa...

Embodiment 2

[0070] Using the method of the present invention to clone the microRNA gene associated with rice yield

[0071] The biological yield and economic yield of rice are directly related to the amount of rice produced by rice, which is controlled by multiple gene loci and interacts with the environment. Through QTL mapping, it was found that more than 2000 loci control rice yield-related traits. microRNA is an important non-coding protein small RNA found in plants, which plays an important regulatory role in plant development, growth and resistance to biotic and abiotic stress, and is also involved in the regulation of rice yield composition.

[0072] 1. Field experiment and trait investigation of rice varieties

[0073] 150 domestic and foreign varieties of various varieties were collected and planted under normal conditions in the field. Seeds of all tested varieties were soaked to accelerate germination to dewy white, then sowed for raising seedlings, and transplanted according...

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Abstract

The invention provides a method for cloning a rice complex trait associated gene. A rice natural population of which the genome sequence is disclosed serves as a base; complex trait data among rice varieties is acquired; ECO-TILLING method analysis is performed on agarose electrophoresis by taking deoxyribonucleic acid (DNA) of the material as a template; generated polymorphism strips are converted into genetic markers; and linkage disequilibrium analysis is performed by association analysis software to determine a gene associated with traits and clone the gene. By the method for cloning the rice complex trait associated gene, the gene associated with the complex traits can be found with high efficiency, low cost and high speed. The method has great significance for quickening clone of the plant complex trait associated gene.

Description

technical field [0001] The invention relates to a method for cloning rice genes, in particular to a method for cloning genes associated with rice complex traits. Background technique [0002] With the completion of more and more plant genome sequencing, cloning and mining related functional genes from a large amount of genome data has become an important work for people. Most of the agronomic traits of crops are complex traits, such as yield, rice quality, 1000-grain weight, flowering period, panicle length, plant height, and drought resistance, etc., which are controlled by the joint action of multiple genes, and the contribution of each gene is relatively small. For the research on this part of traits, at present, most of the genome-wide quantitative trait locus mapping methods are used to find the loci that contribute to this trait in the genome, but to go further to clone related genes, there are still insurmountable obstacles. Including fine mapping, identification of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/10
Inventor 余舜武吴金红黎佳佳廖凤贤罗利军
Owner SHANGHAI AGROBIOLOGICAL GENE CENT
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