Composition for relieving physical fatigue and enhancing immunologic function, preparation method thereof and application thereof
A technology that relieves physical fatigue and enhances immunity. It is applied to medical preparations containing active ingredients, food preparation, drug combinations, etc., and can solve problems such as poor safety, slow onset of action, and low strength.
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Embodiment 1-3
[0116] Compositions 1-3
[0117] Composition A Composition B Composition C Guarana Extract (g) 10 20 75 Taurine (g) 100 200 600 L-lysine hydrochloride (g) 1 2 6 L-Isoleucine (g) 1 2 6 L-Leucine (g) 0.1 0.5 2 L-valine (g) 0.2 1 4 L-Aspartic Acid (g) 1 4 12 L-Tryptophan (g) 0.01 0.04 0.15 L-phenylalanine (g) 0.01 0.04 0.15 L-threonine (g) 0.01 0.04 0.15 L-Methionine (g) 0.01 0.04 0.15 L-alanine (g) 10 20 75 Glycine (g) 10 20 75 Tea polyphenols (g) 1 5 12 American Ginseng Extract (g) 1 6 20
[0118] Various components were mixed according to the above formula to obtain composition 1, composition 2 and composition 3 respectively.
[0119] The preparation method of guarana extract is:
[0120] 1. Guarana seeds were reflux extracted with 6-10 times the amou...
Embodiment 4
[0138] safety test
[0139] Experimental animals: 20 Kunming mice, half male and half male, body weight: 18-22 g, provided by Shanghai Experimental Animal Center, Chinese Academy of Sciences. Production license number: SCXK (Shanghai) 2002-0010, experimental animal breeding room temperature 20-25 ℃, relative humidity 40-70%. Experimental animal license number: SCXK (Shanghai) 2002-0006. Animal feed was provided by Suzhou Shuangshi Experimental Animal Feed Technology Service Co., Ltd., registration number: Su E Si Sheng Zi (2002) 06.
[0140] experimental method:
[0141] (1) Test sample preparation: The daily recommended dose of this product is the total weight of each component of the composition 3 formula in Examples 1-3. Weigh 20 times the weight of composition 3 and add distilled water to 20ml, mix well Homogenized as the test sample.
[0142] (2) Test method: Toxicological safety evaluation is carried out through mouse acute oral toxicity test, mouse bone marrow poly...
Embodiment 5
[0150] Functional test for alleviating physical fatigue
[0151] Experimental animals: Kunming white mice, license number: SCXK (Shanghai) 2002-0010, weighing 18-22 grams, female, clean animals provided by the Shanghai Experimental Animal Center of the Chinese Academy of Sciences (raised in the SPF animal room of this site). Experimental animal breeding room temperature 20-25 ℃, relative humidity 40-70%, experimental animal license number: SCXK (Shanghai) 2002-0006, animal feed, provided by Suzhou Shuangshi Experimental Animal Feed Technology Service Co., Ltd., registration certificate No.: Su E Shi Sheng Zi (2002) 06.
[0152] experimental method:
[0153] (1) Dosage design: In this experiment, three dosage groups of composition 1, composition 2 and composition 3 were set up, and distilled water blank control group and 5.6% sugar control group were also set up.
[0154] (2) Sample treatment: add distilled water to the composition to make up to 20ml, mix well and use it as ...
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