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Method for extracting collagen II

A collagen and extraction method technology, applied in peptide preparation methods, chemical instruments and methods, organic chemistry, etc., can solve the problems of high consumption of solvents and energy, and achieve the effect of simple operation and low energy consumption

Inactive Publication Date: 2011-08-10
YANTAI DONGCHENG PHARMA GRP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the production of type II collagen generally uses a vacuum concentrator to concentrate and add ethanol precipitation, which consumes a lot of solvent and energy.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Example 1, a method for extracting type II collagen is to pulverize 500 grams of bovine nasal bone and add to 5 L of drinking water, add 10 grams of complex protease, hydrolyze for 6 hours, and filter and clarify with a molecular weight cut-off of 8000 Daltons Type II collagen can be obtained from the permeate through the ultrafiltration membrane, and then the permeate is concentrated by an ultrafiltration membrane with a molecular weight cut-off of 1000, and 24 grams of type II collagen are obtained after freeze-drying.

Embodiment 2

[0012] Example 2, a method for extracting type II collagen is to crush 1 kg of shark cartilage and add it to 8 L of drinking water, then add 20 g of 2709 protease and 20 g of AL protease in sequence, hydrolyze for 4 hours, filter and clarify, and then use cut-off molecular weight 30,000 Dalton ultrafiltration membrane treatment, the permeate was concentrated using an ultrafiltration membrane with a molecular weight cut-off of 3,000 Daltons, and 115 grams of type II collagen was obtained after freeze-drying.

Embodiment 3

[0013] Example 3, a method for extracting type II collagen is to add 1,000 kg of chicken breast cartilage to 6,000 L of drinking water, add 10 kg of trypsin and 10 kg of AL protease in turn, hydrolyze for 6 hours, filter and clarify, and use a channel with a cut-off molecular weight of 10,000 Dalton's ultrafiltration membrane treatment, the permeate was concentrated using an ultrafiltration membrane with a molecular weight cut-off of 1000 Daltons, and 61 kg of type II collagen was obtained after spray drying.

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PUM

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Abstract

The invention discloses a method for extracting collagen II. Animal cartilage is subjected to methods of enzymolysis, ultrafiltration, spray drying, and the like to obtain the collagen II. The invention provides a process for comprehensively utilizing the animal cartilage. The method has simple steps and is easy for operation and amplification production. The produced collagen II has the characteristics of high yield and low ash content.

Description

technical field [0001] The invention belongs to the field of biochemical technology, in particular to a method for extracting type II collagen. Background technique [0002] Type II collagen is the main collagen in cartilage, a macromolecule composed of three identical a-chains. Collagen plays an extremely important role in the transmission of information between cells, the maintenance of cell phenotype, the interaction with other matrices, and the overall function of tissues. [0003] Type II collagen is one of the main components of articular cartilage, which plays an important role in maintaining normal human body movement function, joint flexibility, buffering the pressure of body weight and external force on bones and joints. Type II collagen has the function of adjusting the immune function of the human body and inhibiting the attack and destruction of the immune system on its own joints. It can treat rheumatoid arthritis and senile degenerative osteoarthritis from th...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/34
Inventor 曹红光张立勇齐东绮由守谊
Owner YANTAI DONGCHENG PHARMA GRP
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