Phosphate radical sensitive membrane and preparation and using methods thereof
A phosphate radical and sensitive membrane technology, applied in the fields of instruments, material electrochemical variables, analytical materials, etc., can solve the problems of unsuitable replacement of electrodes and high testing costs, and achieve low production costs, reduced manufacturing costs and maintenance costs, and good operation. The effect of stability
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Embodiment 1
[0018] Immerse the nitrocellulose membrane with an average pore size of 0.45 μm and a thickness of 100 μm in a 0.05mol / L, pH value of 6.5 phosphate buffer solution for 6 hours, then take it out, dry it with high-purity nitrogen, and stick it on the O-shaped membrane. On the rubber ring, drop 20 μL of a double-enzyme mixture solution containing 0.30 U / μL maltose phosphorylase and 0.25 U / μL glucose oxidase on the treated nitrocellulose membrane, dry it in a refrigerator at 4 °C for 2 hours, and place it in the refrigerator. 50% glutaraldehyde aqueous vapor cross-linking for 12 hours, and finally immerse the enzyme membrane in 0.05mol / L phosphate buffer solution with a pH value of 6.5 to wash away the loosely bound enzyme, that is, to form The maltose phosphorylase-glucose oxidase dual-enzyme phosphate-sensitive membrane based on the nitrocellulose membrane was stored in a refrigerator at 4°C for use.
[0019] Put the phosphate sensitive film on the top of the platinum electrode ...
Embodiment 2
[0021] Immerse the nitrocellulose membrane with an average pore size of 1.20 μm and a thickness of 150 μm in a 0.05 mol / L, pH value of 6.5 phosphate buffer solution for 12 hours, then take it out, dry it with high-purity nitrogen, and stick it on the O-shaped membrane. On the rubber ring, drop 20 μL of a double-enzyme mixture solution containing 0.15U / μL maltose phosphorylase and 0.125U / μL glucose oxidase on the treated nitrocellulose membrane, dry it in a refrigerator at 0°C for 0.5 hours, and place it in a refrigerator. 50% glutaraldehyde aqueous vapor cross-linking for 18 hours, and finally immerse the enzyme membrane in 0.05mol / L phosphate buffer solution with a pH value of 6.5 to wash away the loosely bound enzyme, that is, to form The maltose phosphorylase-glucose oxidase dual-enzyme phosphate-sensitive membrane based on the nitrocellulose membrane was stored in a refrigerator at 0°C for use.
[0022] Put the phosphate sensitive film on the top of the platinum electrode ...
Embodiment 3
[0024] Immerse the nitrocellulose membrane with an average pore size of 0.20 μm and a thickness of 50 μm in a 0.05mol / L, pH value of 6.5 phosphate buffer solution for 9 hours, then take it out, dry it with high-purity nitrogen, and stick it on the O-shaped membrane. On the rubber ring, drop 20 μL of a double-enzyme mixture solution containing 0.45 U / μL maltose phosphorylase and 0.5 U / μL glucose oxidase on the treated nitrocellulose membrane, and dry it in a refrigerator at 8°C for 1 hour. 50% glutaraldehyde aqueous vapor cross-linking for 6 hours, and finally immerse the enzyme membrane in 0.05mol / L phosphate buffer solution with a pH value of 6.5 to wash away the loosely bound enzyme, that is, to form the following The maltose phosphorylase-glucose oxidase dual-enzyme phosphate-sensitive membrane based on the nitrocellulose membrane was stored in a refrigerator at 8°C for use.
[0025] Put the phosphate sensitive film on the top of the platinum electrode as the working electr...
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