Method for detecting mitochondrial mutations and kit thereof

Abstract

The invention discloses a method for detecting mitochondrial mutations and a kit thereof. The method is used for detecting 1494T and 1555G mutations, five primers are shown as a sequence one, a sequence two, a sequence three, a sequence four and a sequence five in a primer sequence table, the primers are used for multiple polymerase chain reaction (PCR) amplification and melting curve analysis, and whether corresponding mutations happen is judged according to a melting peak at the specific temperature. Meanwhile, the invention also provides a kit special for the method.

Description

technical field [0001] The invention relates to the detection of gene mutation, more specifically, the detection of a mutation of human mitochondrial ribosomal RNA A1555G and C1494T related to drug-induced deafness. technical background [0002] Aminoglycosides such as streptomycin, gentamicin, kanamycin, sisomicin, tobramycin, amikacin, and netilmicin are particularly effective against Gram-negative bacterial infections. It is effective and has a synergistic antibacterial effect on some Gram-positive bacteria, and has become one of the most widely used antibiotics in clinical practice. However, this type of drug has serious ototoxic side effects, and may cause irreversible damage to hearing when used, even It is "one needle for deafness". [0003] This susceptibility is usually maternally inherited, which suggests that its possible pathogenesis is related to mitochondrial DNA (Mitochondrial DNA, mtDNA), and mutations in the mitochondrial 12S rRNA gene are one of the import...

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Problems solved by technology

[0005] For the detection of this mutation, in addition to the direct sequencing method, there are enzyme digestion sequence analysis and Taqman probe method, but these methods are not only expensive, but also cumbersome and long-term. To solve these problems, we designed a simple, fast, Inexpensive assays for clinical testing

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