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Marine organic matter degradation Halomonas sp. strain GHMC414 and application thereof

A technology of GHMC414 and Halomonas, which is applied to the marine organic matter degrading Halomonas strain and its application in the treatment of organic matter-polluted seawater, can solve the problems of secondary pollution, low efficiency of physical methods and high cost, and achieve strong The effect of degradability

Inactive Publication Date: 2011-08-31
SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Current methods of physical and chemical removal of organic pollutants have certain limitations
Among them, the physical method has the disadvantages of low efficiency and high cost; although the chemical method has quick results, it often causes secondary pollution

Method used

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  • Marine organic matter degradation Halomonas sp. strain GHMC414 and application thereof
  • Marine organic matter degradation Halomonas sp. strain GHMC414 and application thereof
  • Marine organic matter degradation Halomonas sp. strain GHMC414 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The screening method of embodiment 1 seawater organic matter degrading bacterial strain:

[0019] 1. Material preparation

[0020] 1. Bacterial source and experimental wastewater

[0021] (1) The source of the bacteria is the sediment of the Shenzhen Dapengao seawater fish cage farm area with a breeding history of more than 20 years.

[0022] (2) Experimental wastewater (sea water containing high-concentration bait organic matter): 5 g of minced fish, 1000 mL of aged sea water, pH 7.6-8.2, sterilized at 121°C for 20 min.

[0023] 2. Medium

[0024] (1) Selection medium: 20g minced fish, 1000mL aged sea water, pH 7.6-8.2, sterilized at 121°C for 20min.

[0025] (2) Separation and slant medium: peptone 5g, yeast extract 1g, ferric phosphate 0.1g, agar powder 20g, aged sea water 1000mL, pH 7.6-8.2.

[0026] (3) Activated expansion medium: peptone 5g, yeast extract 1g, ferric phosphate 0.1g, aged seawater 1000mL, pH 7.6-8.2.

[0027] 3. Experimental instruments and equ...

Embodiment 2

[0049] Example 2 Identification of Marine Organic Matter Degrading Strain GHMC414

[0050] 1. Identification of marine organic matter degradation strain GHMC414

[0051] Physiological and biochemical identification and 16S rDNA molecular identification were carried out on the marine organic matter degradation strain GHMC414, and the species of the strain was determined from the molecular level, combined with the analysis of bacterial morphological characteristics and physiological and biochemical characteristics.

[0052] 16S rDNA sequence analysis mainly follows the following steps:

[0053] ① Extraction of bacterial nuclear DNA:

[0054] 1) Use an autoclaved toothpick to pick a single colony and inoculate it in the expansion medium for overnight culture. Take 1.5 mL of the bacterial solution into an Eppendorf tube, centrifuge at 8000 rpm for 5 min at room temperature, and remove the supernatant completely.

[0055] 2) Add 1.5 mL of STE buffer to wash once, centrifuge to di...

Embodiment 3

[0099] Example 3 Colony morphology, physiological and biochemical characteristics of marine organic matter degradation strain GHMC414

[0100] See Table 1-1 for the colony morphology, physiological and biochemical characteristics of the marine organic matter-degrading strain GHMC414; see Table 1-1 for the cell morphology figure 1 .

[0101] Table 1-1 Colony morphological characteristics and main physiological and biochemical characteristics of marine organic matter degradation strain GHMC414

[0102]

[0103] Note: "+": more than 90% of the strains are positive, "-": more than 90% of the strains are negative.

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Abstract

The invention discloses a marine organic matter degradation Halomonas sp. strain which belongs to Halomonas sp. The preservation name is Halomonas sp. GHMC414, the preservation unit is the China Center for Type Culture Collection, the preservation date is August 28th, 2009, and the preservation number is CCTCC NO: M209183. The strain has efficient organic matter degradation ability, can be used for quickly and efficiently removing organic matters in organic matter polluted sea water and does not have toxic or pathogenic actions on marine aquaculture organisms. The Halomonas sp. strain has the characteristics of safety, environmental protection and high efficiency for removing high-concentration organic matters in the marine aquaculture environment.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a marine organic matter-degrading halomonas strain (Halomonas sp.) and its application in treating seawater polluted by organic matter. Background technique [0002] In recent years, with the rapid development of the sea cage culture industry, the problem of "self-pollution" in the culture has become increasingly prominent. The organic pollutants produced during the culture process often cause the water body of the sea cage culture to stink, and the deposition environment deteriorates, resulting in Dissolved oxygen decreases, farmed fish die, and the ecological balance of water bodies is destroyed. [0003] As a high-density, high-feeding artificial aquaculture ecosystem, the seawater cage aquaculture system is most fundamentally affected by the deposition of organic matter such as a large amount of residual bait, feces, and dead animal and plant debris produce...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C02F103/08
Inventor 古小莉黄洪辉孟霞廖秀丽齐占会
Owner SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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