Antrodia camphorate quick liquid fermentation process based on asexual spores

Abstract

The invention provides an Antrodia camphorate quick liquid fermentation process based on asexual spores. The process is characterized by comprising the following steps of: fermenting Antrodia camphorate serving as parent strains in a fermentation tank to generate the asexual spores; and taking a part of fermentation broth containing the Antrodia camphorate asexual spores to another fermentation tank provided with fresh fermentation media to continuously ferment after the completion of fermentation, wherein material removing and feeding, re-fermentation and circulation can be repeated several times according to requirements. Compared with a batch fermentation process, the quick fermentation process can shorten the fermentation period of the Antrodia camphorate by at least 40 percent and has the characteristics of improvement on production strength, reduction in the probability of bacterial contamination, stabilization of fermentation quality of an Antrodia camphorate product and the like.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a rapid liquid fermentation process of antrodia camphorata based on asexual spores. Background technique [0002] Antrodia camphorata (Antrodia camphorata), commonly known as Antrodia camphorata and Antrodia camphorata, is a unique medicinal fungus in Taiwan (Polyporaceae, Phyllobacteriaceae), with a high degree of host specificity. The interior of the core material of the broad-leaved tree camphor tree. For a long time, Antrodia camphorata has been used by folks to relieve alcohol and protect the liver, treat liver pain, food poisoning, abdominal pain, diarrhea, inflammation, skin itching and liver cancer, and has good curative effects. Modern pharmacological studies have confirmed that the fruiting body of Antrodia camphorata and its fermentation products have unique effects in protecting the liver, anti-oxidation, and treating liver cancer. So far, more th...

AI Technical Summary

Problems solved by technology

From the research results, the existing Antrodia camphorata submerged culture technology mainly has the following problems: 1) Compared with other microorganisms, the fermentation cycle of Antrodia camphorata is longer

Most of the time for the fermentation stage of Antrodia camphorata mycelium needs more than ten days, and it will take longer if the time for strain activation and fermentation seed preparation is added; Difference

The traditional Antrodia camphorata fermentation adopts the inoculation method of digging mycelia on an inclined plane, which is likely to cause problems such as uneven inoculation and unstable quality of seed batches.

In addition, the filamentous hyphae of Antrodia camphorata are easily entangled to form mycelial balls during the fermentation process, and the mass transfer, heat transfer, and dissolved oxygen inside and outside the balls are generally different.

Therefore, the controllability of the entire Antrodia camphorata fermentation process is relatively poor, which eventually leads to unstable quality of the Antrodia camphorata fermentation product; 3) Because the fermentation time of Antrodia camphorata is long, after the fermentation is completed, it will be cleaned, emptied, and the culture medium is prepared. Inoculate after clearing and cooling. The non-production cleaning time takes too long, which affects production efficiency.

At the same time, due to the long production cycle and many process links, pollution is prone to occur during the fermentation process, and the requirements for fermentation equipment are high, so the production cost is high and the quality of the fermentation product is also affected.