Antrodia camphorate quick liquid fermentation process based on asexual spores

A fermentation process and asexual spore technology, applied in the field of bioengineering, can solve the problems of long production cycle, long fermentation time of Antrodia camphorata, and excessive cleaning time, so as to improve the quality of fermentation products, reduce the chance of contamination by miscellaneous bacteria, and reduce costs Effect

Active Publication Date: 2011-09-07
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

From the research results, the existing Antrodia camphorata submerged culture technology mainly has the following problems: 1) Compared with other microorganisms, the fermentation cycle of Antrodia camphorata is longer
Most of the time for the fermentation stage of Antrodia camphorata mycelium needs more than ten days, and it will take longer if the time for strain activation and fermentation seed preparation is added; Difference
The traditional Antrodia camphorata fermentation adopts the inoculation method of digging mycelia on an inclined plane, which is likely to cause problems such as uneven inoculation and unstable quality of seed batches.
In addition, the filamentous hyphae of Antrodia camphorata are easily entangled to form mycelial balls during the fermentation process, and the mass transfer, heat transfer, and dissolve

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Rapid fermentation technology of Antrodia camphorata shake bottle

[0022] 1. Bacterial strain: the strain of Antrodia camphorata (Antrodia camphorata) whose preservation number is ATCC No. 200183 is used as the starting strain.

[0023] 2. Slant culture: transfer the Antrodia camphorata mycelia to the slant medium, and culture at 28°C for 288 hours.

[0024] Incline medium (g / L): potato 200, glucose 20, agar 20, pH natural.

[0025] 3. Seed shake flask culture: transfer the slant strain into a 500 mL shake flask filled with 100 mL shake flask culture medium, cultivate at 26° C., rotate at 100 rpm, and cultivate for 72 hours.

[0026] Shake flask medium (g / L): glucose 20, peptone 2, magnesium sulfate 0.75, potassium dihydrogen phosphate 1.5, bran 2, pH 4.5.

[0027] 4. Fermentation shake flask culture: Antrodia camphorata fermented liquid was transferred into a 500mL fermentation shake flask equipped with 100mL fermentation medium according to 15% inoculation amount, ...

Embodiment 2

[0032] Rapid Fermentation Process of Antrodia Camphorata Fermentation Tank

[0033] 1. Bacterial species: Antrodia camphorata (Antrodia camphorata) strain with preservation number BCRC No. 35398 was used as the starting bacterial species.

[0034] 2. Slant culture: transfer the Antrodia camphorata mycelia to the slant medium, and culture at 26° C. for 312 hours.

[0035] Slant medium (g / L): potato 180, glucose 18, agar 18, natural pH.

[0036] 3. Seed shake flask culture: transfer the slanted strain of Antrodia camphorata into a 500 mL shake flask filled with 100 mL shake flask culture medium, culture at 26°C, rotate at 100 rpm, and cultivate for 48 hours.

[0037] Shake flask medium (g / L): glucose 20, peptone 2, magnesium sulfate 0.5, potassium dihydrogen phosphate 1, bran 2, pH 4.5.

[0038] 4. Fermentation in seed tank: Transfer Antrodia camphorata fermented liquid into a seed tank equipped with seed culture medium according to 15% inoculum amount, cultivate at 26° C., ro...

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Abstract

The invention provides an Antrodia camphorate quick liquid fermentation process based on asexual spores. The process is characterized by comprising the following steps of: fermenting Antrodia camphorate serving as parent strains in a fermentation tank to generate the asexual spores; and taking a part of fermentation broth containing the Antrodia camphorate asexual spores to another fermentation tank provided with fresh fermentation media to continuously ferment after the completion of fermentation, wherein material removing and feeding, re-fermentation and circulation can be repeated several times according to requirements. Compared with a batch fermentation process, the quick fermentation process can shorten the fermentation period of the Antrodia camphorate by at least 40 percent and has the characteristics of improvement on production strength, reduction in the probability of bacterial contamination, stabilization of fermentation quality of an Antrodia camphorate product and the like.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a rapid liquid fermentation process of antrodia camphorata based on asexual spores. Background technique [0002] Antrodia camphorata (Antrodia camphorata), commonly known as Antrodia camphorata and Antrodia camphorata, is a unique medicinal fungus in Taiwan (Polyporaceae, Phyllobacteriaceae), with a high degree of host specificity. The interior of the core material of the broad-leaved tree camphor tree. For a long time, Antrodia camphorata has been used by folks to relieve alcohol and protect the liver, treat liver pain, food poisoning, abdominal pain, diarrhea, inflammation, skin itching and liver cancer, and has good curative effects. Modern pharmacological studies have confirmed that the fruiting body of Antrodia camphorata and its fermentation products have unique effects in protecting the liver, anti-oxidation, and treating liver cancer. So far, more th...

Claims

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Application Information

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IPC IPC(8): A01G1/04
Inventor 许正宏陆震鸣史劲松许泓瑜窦文芳钱建瑛
Owner JIANGNAN UNIV
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