Unlock instant, AI-driven research and patent intelligence for your innovation.

Culture dish containing micro spherical arrays and application thereof

A petri dish and microsphere technology, which is applied in the field of petri dishes containing microsphere arrays, to achieve the effects of improving fluorescence responsiveness, low production cost, and increasing functional responsiveness

Inactive Publication Date: 2011-09-07
CHONGQING UNIV
View PDF4 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the method of genetic engineering can achieve the purpose of enhancing the functional responsiveness of VDCC and constructing screening cells, the impact of genetic manipulation on the screening results is worthy of further evaluation

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Culture dish containing micro spherical arrays and application thereof
  • Culture dish containing micro spherical arrays and application thereof
  • Culture dish containing micro spherical arrays and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1 Preparation of Petri dishes containing microsphere arrays

[0039] One material preparation:

[0040] 1. 35 mm cell culture dish (Corning, USA); cell culture six-well plate (Nest Biotech Co., Ltd, China)

[0041] 2. Circular coverslips with a diameter of 25 mm (Shanghai Jinglun Industrial Glass Co., Ltd., China. or Fisher Scientific, USA)

[0042] 3. Polystyrene microspheres (Bangs Laboratories, Inc., USA. or Aladdin, China) with diameters of 0.51 μm and 1.98±0.20 μm, respectively.

[0043] 4. 2.5% polystyrene solution. Take an ordinary polystyrene cell culture dish, cut it into pieces, weigh 2.5 grams of polystyrene, and add 100 mL of toluene to dissolve it. The dissolved polystyrene solution was placed in a dark bottle and kept away from light for later use.

[0044]5. 10.16% polystyrene solution. Take an ordinary polystyrene cell culture dish, cut it into pieces, weigh 10.16 grams of polystyrene, and add 100 mL of toluene to dissolve it. The dissolv...

Embodiment 2

[0083] Example 2 Method for Enhancing Functional Responsiveness of Cell Voltage-Dependent Calcium Channels Containing Microsphere Arrays in Petri Dishes

[0084] The above culture dish containing the micro-global spherical array was subjected to cell culture and evaluation of the functional responsiveness of the voltage-dependent calcium channel to verify the implementation effect of the method. It should be pointed out that for the petri dish of the micro-spherical array, the topological microenvironment perceived by nerve cells is exactly the same as that of the micro-spherical array, because the scale and growth behavior of the cells determine that it is impossible for the cells to attach to the microsphere and the micro-spherical array. The bottom thus perceives the difference in the geometry of this part. The inventors have every reason to speculate that the hemispherical spherical dense array topology has the same cell biological effects as the micro-spherical dense arra...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
areaaaaaaaaaaa
Login to View More

Abstract

The invention relates to the field of bio-nanometer, in particular to a culture dish containing micro spherical arrays. The culture dish comprises a culture dish body 1 and a culture dish cover plate 2, wherein the surface of a base 3 of the culture dish body 1 is provided with at least one micro spherical array 4, and the diameter of a microsphere 5 in the micro spherical array 4 is of micron or nanometer grade. The invention also comprises a method for enhancing the voltage compliance type calcium ion channel functional responsibility of cells, which specifically comprises two steps of pre-treating with the culture dish and enhancing the voltage compliance type calcium ion channel functional responsibility of cells. The culture dish is prepared by a step-by-step etching method under an inclination condition; the micro spherical arrays of the culture dish are of micron or nanometer grade; the area of the prepared micro spherical arrays meets the requirements of culture dish application; and the culture dish has low production cost and is simple to operate. The method for enhancing the voltage compliance type calcium ion channel functional responsibility of cells by using the culture dish provides a new method and a new thought for drug screening.

Description

technical field [0001] The invention relates to the field of biological nanometers, in particular to a petri dish containing a microsphere array and its application. Background technique [0002] Cell-based biological detection is an important functional screening method for medicinal effects or toxicological effects developed in the field of drug screening in the past two decades. In this screening system, cells are integrated into the screening platform as a biosensor, which can be used as a detection method for high throughput screening (HTS) or high content screening (HCS) . Compared with biochemical detection methods, cell screening methods have the advantage of obtaining the medicinal effects or toxicological effects of the molecules to be screened from the functional point of view, but the screening costs are significantly lower than animal experiments. [0003] In the existing cell drug screening system, ordinary flat petri dishes are usually used for cell culture....

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/22C12N5/00
CPCC12M23/10C12M35/08C12M41/32
Inventor 吴泽志宋兆全朱满根林雨廖彦剑黄岂苹金良梁福荣
Owner CHONGQING UNIV