Method and device for detecting bacteria

A technology for bacteria detection and bacteria, applied in transmittance measurement, color/spectral characteristic measurement, fluorescence/phosphorescence, etc., can solve the problems such as the inability to meet the needs of rapid detection of water bodies, the inability to achieve accurate quantification of bacteria, and the great influence of water body background, etc. The effect of reducing system replacement and maintenance costs, improving the coincidence rate of results, and improving detection accuracy

Inactive Publication Date: 2011-09-07
FOCUSED PHOTONICS +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] 1. Low concentration and long detection time
[0008] The above detection method is to directly sample from the water body and culture the sample for detection, and the detection time is related to the initial amount of bacteria in the water body: if the amount of bacteria in the water body is small, the detection will take a long time and the detection efficiency is low. In this case, the above method cannot meet the rapid detection requirem

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  • Method and device for detecting bacteria
  • Method and device for detecting bacteria
  • Method and device for detecting bacteria

Examples

Experimental program
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Effect test

Embodiment 1

[0056] See figure 1 , A bacteria detection device for detecting bacteria in water samples; the bacteria detection device includes a sampling unit, a filtering unit 1 and a detection unit 2;

[0057] The sampling unit includes a flow path switching module and a pipeline, the flow path switching module includes a pump and a valve; the flow path switching module is respectively connected to the filter unit 1, the sample to be tested, and the reagent through the pipeline, for selecting Sexually supply the test sample and reagent to the filter unit; in this embodiment, the reagent is a rinse liquid;

[0058] The pump includes a pump 311 and a pump 312 to drive the sample into the filter unit 1 to filter or clean the filter unit 1. The pump can be a peristaltic pump, a diaphragm pump, a syringe pump, and other fluid driving devices;

[0059] The valve includes a valve 321, a valve 322, a valve 325, and a valve 326, which are arranged on the pipeline to control the on and off of the pipelin...

Embodiment 2

[0100] See figure 2 , A bacteria detection device, which is different from the bacteria detection device described in embodiment 1: the bacteria detection device of this embodiment further includes a pressure monitoring member 4, which is arranged on the pipeline between the filter unit and the detection unit to monitor the filter unit Pressure of the flow path;

[0101] The hollow fiber filter module is composed of 400 hollow fiber membranes 121 made of hydrophilic polysulfone filter membranes, which can bear a pressure of 0.2 MPa; the hollow fiber membrane 121 has an inner diameter of 400 μm and an outer diameter of 600 μm. The equivalent filter pore size of the wall is 0.2μm;

[0102] The flow path switching module further includes a valve 323 and a valve 324. The reagent also includes a cleaning solution and a disinfecting solution; the valve 323 is connected to the pump 311, the pump 312, and the disinfecting solution, respectively, and the valve 324 is connected to the pump ...

Embodiment 3

[0121] See image 3 , A bacteria detection device, which is different from the detection device described in Example 2 in that: the pressure monitoring member 4 is set on the pipeline between the sampling unit and the filter unit to monitor the pressure of the flow path of the filter unit. This embodiment is set in Between the pump 311 and the injection port 12;

[0122] The hollow fiber filter module is composed of 500 hollow fiber membranes 121 made of hydrophilic polysulfone filter membrane;

[0123] The detection module and the analysis module are arranged in the incubator; the light emitting part 322 and the light receiving part 323 are arranged on opposite sides of the incubation chamber 221 in the incubator, and can emit and detect light with a wavelength of 405 nm;

[0124] The disinfectant in this embodiment is 0.5% of DCC sodium salt, namely sodium dichloroisocyanurate.

[0125] This embodiment also provides a bacteria detection method. The difference from the detection meth...

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Abstract

The invention relates to a method for detecting bacteria, comprising the following steps of: a, sampling step: feeding a to-be-detected sample into a hollow fiber filtering unit, intercepting bacteria and/or bacterial metabolites by a hollow fiber membrane and accumulating the bacteria and/or bacterial metabolites in the filtering unit; feeding the flushing liquid into the filtering unit, flushing the inner side and/or the outer side of the hollow fiber membrane, flushing out the accumulated bacteria and/or the bacterial metabolites; and b, culturing and detecting step: culturing the bacteria and/or the bacterial metabolites which are eluted by the filtering unit, detecting the change of solution in a culturing room to obtain the information of target bacteria in the to-be-detected sample. The invention further provides a device for detecting bacteria, which comprises a sampling unit, a filtering unit and a culturing and detecting unit. The method has the advantages of short detecting time, improved detecting efficiency, high detection accuracy; and the detecting device has long service life and low maintaining cost.

Description

Technical field [0001] The invention relates to the detection of bacteria, in particular to a method and device for automatically intercepting and detecting bacteria based on a specific reaction substrate method. Background technique [0002] Surface water, groundwater, and even rainwater contain many kinds of bacteria. Especially when the water body is polluted by human and animal manure, domestic sewage or some industrial and agricultural wastewater, the bacteria in the water body will increase greatly. Therefore, the bacteriological test of water, especially the test of intestinal bacteria, is of great significance in hygiene. Among them, total coliforms, heat-resistant coliforms, and Escherichia coli have been widely used as important routine microbiological testing indicators for water safety assessment. Through the detection of them and other bacteria, the sanitary quality of water can be judged and people can drink water. Safety. [0003] In the existing water bacteria de...

Claims

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Application Information

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IPC IPC(8): G01N21/31G01N21/59G01N21/64
Inventor 忻鼎丞项光宏
Owner FOCUSED PHOTONICS
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