Unlock instant, AI-driven research and patent intelligence for your innovation.

Process for extracting radix achyranthis bidentatae triterpenoid saponins

A kind of achyranthes triterpenoid saponin and extraction process technology, which is applied in the field of extraction process of achyranthes triterpenoid saponin, can solve the problems of organic solvent toxicity residue and high cost, and achieves the effects of short extraction time, low cost and high yield

Inactive Publication Date: 2011-09-14
NANJING ZELANG MEDICAL TECH
View PDF1 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are few extraction and purification methods for Achyranthes saponins, and alcohol extraction and n-butanol extraction are mostly used. This method has the disadvantages of high cost and toxic residual organic solvents.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Grind Achyranthes bidentata medicinal material, take 1kg, add 5 times hot water, the temperature of hot water is 100°C, ultrasonic extraction, frequency 45kHz, extraction 4 times, 1 hour each time, filter, combine extracts, and pass through AB-8 macropores Adsorption resin column chromatography, elute impurities with 3L distilled water, then elute with 15%, 30%, 45%, 60% methanol solution, and detect with thin layer chromatography, collect 40-60% alcohol solution eluate , add 0.5% (w / v) medicinal activated carbon, heat to reflux, filter while it is hot, concentrate the filtrate to obtain an extract, dissolve in hot water, place crystals on place, filter, dissolve the crystals with absolute ethanol under reflux, let cool to crystallize, and filter out Crystallize and spray dry to obtain high-purity Achyranthes bidentata triterpene saponins.

Embodiment 2

[0021] Grind Achyranthes bidentata medicinal material, take 2kg, add 12 times hot water, the temperature of hot water is 80°C, ultrasonic extraction, the frequency is 35kHz, extraction 2 times, 2 hours each time, filter, combine the extracts, and pass through X-8 macropores Adsorption resin column chromatography, elute impurities with 10L distilled water, then elute with 20%, 30%, 40%, 50% ethanol solution, and detect with thin layer chromatography, collect 40-60% alcohol solution eluate , add 0.6% (w / v) medicinal activated carbon, heat to reflux, filter while it is hot, concentrate the filtrate to obtain an extract, dissolve in hot water, place crystals on place, filter, dissolve the crystals with absolute ethanol under reflux, let cool to crystallize, and filter out Crystallize and spray dry to obtain high-purity Achyranthes bidentata triterpene saponins.

Embodiment 3

[0023] Grind the Achyranthes bidentata medicinal material, take 2kg, add 6 times hot water, the temperature of the hot water is 60°C, ultrasonic extraction, the frequency is 30kHz, extraction 3 times, each time 1 hour, filter, combine the extracts, pass through D180 macroporous adsorption resin Column chromatography, elute impurities with 12L distilled water, then elute with 15%, 30%, 40%, 60% ethanol solution, and use thin layer chromatography to detect, collect 40-60% alcohol solution eluate, add 0.8% (w / v) medicinal activated carbon, heated to reflux, filtered while hot, concentrated filtrate to obtain extract, dissolved in hot water, placed crystals, filtered, refluxed with absolute ethanol to dissolve the crystals, let the crystals cool, filtered out the crystals, High-purity Achyranthes bidentata triterpene saponins can be obtained by spray drying.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a process for extracting radix achyranthis bidentatae triterpenoid saponins, which comprises the steps of: crushing radix achyranthis bidentatae, ultrasonically extracting with hot water as an extracting solvent, enabling an extraction solution to pass through a macropore polymeric adsorbent resin column, eluting with a water and alcohol solution, checking with a thin layer chromatography, collecting 40-60 percent alcohol solution eluent, decoloring with active carbon, concentrating a decoloring solution to obtain extract, dissolving the extract with hot water, placing for crystallization, recrystallizing absolute ethyl alcohol, filtering crystals, spraying and drying to obtain high-purity radix achyranthis bidentatae triterpenoid saponins. The invention has the advantages of no pollution in a production process, simple extraction method and easiness in amplifying production.

Description

Technical field: [0001] The invention belongs to the technical field of natural medicine extraction, and in particular relates to an extraction process of Achyranthes bidentata triterpene saponins. Background technique: [0002] Radix Achyranthis Bidentatae is the root of Radix Achyranthis Bidentatae. It has the effects of invigorating the liver and kidney, strengthening the bones, promoting blood circulation, activating the fire, diuresis and treating stranguria. It is used for soreness of the waist and knees, flaccidity of the lower limbs, amenorrhea due to blood stagnation, dysmenorrhea, postpartum blood stasis, abdominal pain, abdominal pain, lumps, retained clothes, fever Drenching, bloody stranguria, bruises, carbuncle and malignant sores, sore throat and other diseases. The root contains saponins, mainly triterpene saponins, and contains immune active peptidoglycan of Achyranthes bidentata etc. Wang Lijing published "Achyranthes bidentata fingerprints and extraction...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K36/21A61P11/04A61P15/00A61P17/02A61P29/00A61K125/00
Inventor 刘东锋万冬梅杨成东
Owner NANJING ZELANG MEDICAL TECH