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Bacterial strain B1 for converting insoluble phosphate into soluble phosphate

A kind of insoluble phosphate, soluble technology, applied in the biological field, can solve the problem of phosphorus dissolution restriction, unstable phosphorus dissolution and other problems

Inactive Publication Date: 2011-09-21
NANJING AGRICULTURAL UNIVERSITY
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AI Technical Summary

Problems solved by technology

However, the phosphorus-dissolving effect of these isolated microorganisms is not stable, and their phosphorus-dissolving effect is restricted by environmental conditions.

Method used

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  • Bacterial strain B1 for converting insoluble phosphate into soluble phosphate
  • Bacterial strain B1 for converting insoluble phosphate into soluble phosphate
  • Bacterial strain B1 for converting insoluble phosphate into soluble phosphate

Examples

Experimental program
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Effect test

Embodiment Construction

[0023] 1 Isolation and identification of strains

[0024] Take the soil suspension of Jinxian tea garden soil in Jiangxi, and carry out gradient dilution. The diluted solution was spread on the improved PKO solid medium and cultured at 30°C for 4 days. Pick a single colony to verify its phosphorus-dissolving effect, and save the strain with a larger phosphorus-dissolving circle and a better phosphorus-dissolving effect in liquid shake flask culture for subsequent experiments. See the picture of its colony figure 1 .

[0025] The formula of the improved PKO solid medium is: per liter, it contains 4g of aluminum phosphate, 10g of sucrose, 0.5g of ammonium sulfate, 0.1g of sodium chloride, 0.1g of magnesium sulfate heptahydrate, 0.2g of potassium chloride, 0.03g of manganese sulfate, seven Hydrated ferrous sulfate 0.03g, yeast extract 0.5g, agar 20g, pH 7.0.

[0026] The strain B1 was sequenced by Nanjing GenScript Biotechnology Co., Ltd. According to the sequencing results o...

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Abstract

The invention provides a bacterial strain B1 for converting an insoluble phosphate into a soluble phosphate, which belongs to the technical field of biology. The scientific name of the bacterial strain is bacillus thuringiensis which is preserved in the general microorganism center of China Committee for Culture Collection of Microorganisms on 15th, March, 2011, and the preservation number is No.4665. The bacterial strain employs the insoluble phosphate as the phosphorus source, and the insoluble phosphate can be converted into the soluble phosphate, the effective phosphor content in the soil can be effectively raised. The bacterial strain is directly inoculated into the soil with good survivability, and the effective phosphor content in the soil can be effectively raised. According to the invention, the bacterial strain has the advantages of low production cost, convenient usage and good soil survivability, and is suitable for an inoculation test in phosphate deficiency soil. The invention has significance for reducing the agricultural production cost.

Description

technical field [0001] The invention provides a bacterial strain B1 capable of converting poorly soluble phosphate into soluble phosphate, belonging to the field of biotechnology. It uses microorganisms to convert insoluble phosphate in red soil into available phosphorus that can be absorbed by plants, and is suitable for red soil areas that are generally deficient in phosphorus. Background technique [0002] Phosphorus is an important limiting factor in agroecosystems, and improving soil P availability will be of strategic importance. There are a large number of phosphorus-dissolving bacteria and phosphorus-dissolving fungi in the soil, which can promote the dissolution of insoluble phosphate in soil and the mineralization of organic phosphorus. Undoubtedly, it is an important way to improve soil availability by using biological means. In recent years, due to the increasingly prominent ecological and environmental problems caused by the extensive use of water-soluble phosp...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P9/00C12R1/07
Inventor 王同李辉信胡锋焦加国刘满强
Owner NANJING AGRICULTURAL UNIVERSITY
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