Method for separation and extraction of active peptide from domestic fungus by-products

A separation method and edible fungus technology, applied in the preparation method of peptides, chemical instruments and methods, peptides, etc., can solve the problems of lack of pertinence, difficulty in separation, low purity, etc., and achieve improved utilization rate, simple process, and hydrolysis time short effect

Inactive Publication Date: 2011-09-28
梅跃明
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The purpose of the present invention is to adopt a simple and easy method to produce active peptides of edible fungi, overcome the shortcomings of the existing technology such as roughness, lack of pertinence, large investment, high cost, low purity, and difficult separation. Enzymatic hydrolysis combined with membrane separation technology to prepare active peptides, with mild reaction, simple process, rich peptide content, and significantly better quality than similar products

Method used

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  • Method for separation and extraction of active peptide from domestic fungus by-products

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Embodiment 1

[0037] Wash it with Flammulina velutipes, mash it, and grind it into a paste, so that the weight ratio of water and material is 1:15, the pH value is adjusted to 9, and the temperature is 50°C. Add alkaline protease, papain, and trypsin in a weight ratio of 1 : 1:1, the concentration weight ratio of the substrate material to the compound enzyme is 3%, after the enzymolysis time is 3 hours, the enzymolysis solution is heated to 90°C, kept for 20min, the enzyme is extinguished, and the enzymolysis solution is centrifuged at the same time; The liquid is pre-filtered through a plate and frame filter, and the filter material is nylon filter cloth; the coarse filtrate is fine-filtered through a cellulose acetate organic membrane with a cut-off particle size of 0.1 μm, and the filtration pressure is 0.15 MPa; Amide organic composite membrane ultrafiltration, filtration pressure 0.2MPa, cut off and harvest peptide solution with molecular weight less than 8000Da; peptide solution vacuum...

Embodiment 2

[0039] Wash it with shiitake mushrooms, smash them, and grind them into a paste, so that the weight ratio of water and materials is 1:12, the pH value is adjusted to 7, and the temperature is 35°C. Add alkaline protease, papain, and trypsin in a weight ratio of 1 : 1:1, the concentration weight ratio of the substrate material to the compound enzyme is 3%, after the enzymolysis time is 4 hours, the enzymolysis solution is heated to 85°C, kept for 18min, the enzyme is extinguished, and the enzymolysis solution is centrifuged at the same time; The liquid is pre-filtered through a plate and frame filter, and the filter material is nylon filter cloth; the coarse filtrate is fine-filtered through a cellulose acetate organic membrane with a cut-off particle size of 0.2 μm, and the filtration pressure is 0.2 MPa; the fine filtrate is then passed through an aromatic polyamide with a molecular weight cut-off of 6000Da Organic composite membrane ultrafiltration, filtration pressure 0.25MP...

Embodiment 3

[0041] Wash it with Flammulina velutipes, mash it, and grind it into a paste, so that the weight ratio of water and material is 1:12, the pH value is adjusted to 8, and the temperature is 55°C. Add alkaline protease, papain, and trypsin in a weight ratio of 1 : 1:1, the weight ratio of the substrate material to the compound enzyme is 3%, after the enzymolysis time is 5 hours, the enzymolysis solution is heated to 80°C, kept for 15min, the enzyme is extinguished, and the enzymolysis solution is centrifuged at the same time; the enzymolysis solution is taken After primary filtration by plate and frame filter, the filter material is nylon filter cloth; the coarse filtrate is finely filtered by cellulose acetate organic membrane with a cut-off particle size of 0.15 μm, and the filtration pressure is 0.2 MPa; the fine filtrate is then passed through an aromatic polyamide with a molecular weight cut-off of 6000Da Organic composite membrane ultrafiltration, filtration pressure 0.15MPa...

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Abstract

The invention aims to prepare domestic fungus active peptide by a method combining composite enzyme enzymolysis with membrane separation technology. The method is characterized by mild reaction and simple process. And products of the method contain abundant peptide. The method comprises the steps of: pretreating domestic fungus by-products like golden mushroom, pleurotus eryngii, mushroom and the like, adding alkali protease, papain and trypsin in a ratio of 1-1.2:1-1.2:1-1.2 by weight, performing enzymolysis to the composite enzyme for 3-5 hours, killing enzyme, filtering and centrifuging the enzymolyzed liquid, conducting membrane separation to the obtained crude extract from last step, under a filtration pressure of 0.15-0.2 MPa implementing fine filtration to primary filtrate with an acetate fiber organic membrane with an intercepting particle size of 0.1-0.2 micrometers, under a filtration pressure of 0.15-0.25 MPa performing hyperfiltration to fine filtrate with an aromatic polyamide organic composite membrane with a cut-off molecular weight less than 8000 Da so as to get peptide liquid with a molecular weight less than 8000 Da, conducting vacuum concentration and spray drying to the peptide liquid, thus obtaining extracted domestic fungus active peptide.

Description

technical field [0001] The invention belongs to an extraction method of active peptides, in particular to a technology for extracting active peptides by using edible fungus by-products. Background technique [0002] Peptides are a class of biologically active compounds whose molecular structure is between amino acids and proteins. Studies have shown that: it can regulate and inhibit enzymes in organisms, and has immune regulation, antithrombotic, antihypertensive, lowering cholesterol, and inhibiting bacteria , virus, anti-cancer, anti-oxidation, scavenging free radicals, etc. Therefore, active peptides are a treasure house of natural resources for screening drugs, preparing vaccines and food additives, and have been increasingly valued by people in various countries in recent years. [0003] In recent years, studies have found that protein is mainly absorbed in the form of small peptides after enzymatic hydrolysis in the digestive tract, which is easier and faster to be abs...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/34
CPCY02P20/582
Inventor 梅跃明
Owner 梅跃明
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