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Method for producing xylose with straw

A technology of xylose and straw

Inactive Publication Date: 2011-10-12
山西省生物研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The present invention is to solve the problems that the traditional xylose production process is complicated, the amount of ion exchange resin is large, the equipment required is large, the cost is high, the environment is easy to be polluted, the sugar liquid produced has a high viscosity, and the yield of xylose crystallization is low.

Method used

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Embodiment Construction

[0016] A method for producing xylose from stalks, the production steps are as follows:

[0017] (1) the required actinomycete bacterial strain of the present invention is obtained by following way: collect sample from the cornfield compost of Yuci Suburb, Shanxi, adopt conventional microbial separation and screening technology, get the sample of 1g and place in 99ml sterile water and carry out gradient dilution, And absorb 0.1ml~0.5ml of the dilution and spread it on the hemicellulolytic bacteria screening plate, the pH value of the hemicellulolytic bacteria screening medium is 6.5, and the hemicellulolytic bacteria screening medium consists of the following components by weight Composition: (NH 4 ) 2 SO 4 0.5g, K 2 HPO 4 1.0g, MgSO 4 ·7H 2 O 0.3g, CaCl 2 2H 2 O 0.2g, K 2 SO 4 0.1g, NaCl 0.2g, hemicellulose 15g, tap water 1000ml, incubate at 36°C for 72h, select the strain with large colony transparent circle to ferment and degrade hemicellulose, and select multip...

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Abstract

The invention discloses a method for degrading the hemicellulose prepared from straw by utilizing the xylanase which is fermented by utilizing actinobacteria. The technical scheme in the invention comprises four main working procedures, namely the preparation of xylanase, the preparation of hemicellulose, the enzymolysis of hemicellulose and the purification and refinement of xylose. The enzymatic method is adopted to produce xylose; and the method has the following advantages: the specificity is high, the conditions are mild, the impurities of the intermediate are fewer, the intermediate is easy to purify and extract, the conversion rate of hemicellulose and the yield of xylose crystals are higher, clean and environmentally-friendly production can be maintained in industry, and the like. The total raw material conversion rate is more than 15% and the xylose content of the finished product is more than 98.5%.

Description

Technical field: [0001] The invention belongs to a production method of xylose, in particular to a method for producing xylose by utilizing straw. Background technique: [0002] Xylose is a white needle-like crystalline powder with a sweet taste and a sweetness equivalent to 40% of sucrose. It is easily soluble in water, with a melting point of 147-151°C. It has dextrorotatory and mutarotropic properties. [0003] As a base material of functional food, xylose has the following main functions: (1) It is not digested and absorbed, has no energy value, and can satisfy the needs of those who love desserts but are worried about gaining weight to the greatest extent; (2) Activate the human intestinal tract (3) It is not used by microorganisms in the oral cavity and can prevent dental caries; (4) It has some physiological functions of dietary fiber, and has the effects of lowering blood fat, lowering cholesterol and preventing intestinal cancer; ( 5) The compatibility of xylose a...

Claims

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Application Information

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IPC IPC(8): C12P19/14C12R1/01
Inventor 彭晓光杨庆文杨林娥杨宇霞韩建欣丁玉华吴光明
Owner 山西省生物研究所
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