Preparation method and application of bitter gourd dietary fiber

A technology of dietary fiber and bitter gourd, which is applied in the field of preparation of bitter gourd dietary fiber, to achieve the effect of obvious regulation of blood sugar, wide source and obvious effect

Inactive Publication Date: 2011-10-26
三沙南海美源岛生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, there are relatively few reports about the dietary fiber of bitter gourd, and it is reported in CN1826976A that a compound enzyme is used: composed of papain and amylase, to extract dietary fiber of bitter gourd. The purity of dietary fiber of bitter gourd reported by this method is more than 95%, but the dietary fiber is Insoluble, not specifically extracted for soluble dietary fiber

Method used

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  • Preparation method and application of bitter gourd dietary fiber

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Embodiment 1

[0027] Take 1000.0g of bitter gourd, wash it dry, cut into sections, add 10L of water to make a slurry, pass the slurry through a colloid mill for 3 times, and pass through a high-pressure homogenization for 3 times. Heat to 100°C for 180 min, then cool down to room temperature. Ultrasonic treatment, the treatment conditions are 5000W, 90min. Adjust the pH value to 7.5 with 10 mol / L NaOH solution, and add 20,000 international units (specific activity) of neutral protease for enzymolysis at 50°C for 500 minutes. After protease hydrolysis, adjust the pH value to 7.5 with 10mol / L NaOH solution, heat up to 100°C, and add 20,000 international units (specific activity) of amylase for enzymolysis for 180 minutes. After enzymatic hydrolysis, heat to 95°C for 10 minutes, then cool down to room temperature. Centrifuge and filter to obtain 4.8L of filtrate and filter residue. Take 4.8L of the filtrate and add 95% ethanol to a final concentration of 85%, let stand overnight, centrifuge...

Embodiment 2

[0033] Take 1000.0g of bitter gourd, wash it dry, cut into sections, add 4L of water to make a slurry, pass the slurry once through a colloid mill, and once through a high-pressure homogenization. Heat to 80°C for 30 minutes, then cool down to room temperature. Ultrasonic treatment, the treatment conditions are 500W, 20min. Adjust the pH value to 6.5 with 10 mol / L NaOH solution, and add 1,000 international units (specific activity) of protease for enzymolysis at 40°C for 180 minutes. After the enzymatic hydrolysis with neutral protease, adjust the pH value to 6.5 with 10 mol / L NaOH solution, heat to 50°C, and add 0.1 thousand international units (specific activity) of amylase for enzymatic hydrolysis for 30 minutes. After enzymatic hydrolysis, heat to 95°C for 10 minutes, then cool down to room temperature. Centrifuge and filter to obtain 2.2L of filtrate and filter residue. Take 2.2L of the filtrate and add 95% ethanol to a final concentration of 60%, let it stand overnigh...

Embodiment 3

[0039] Take 1000.0g of bitter gourd, wash it dry, cut into sections, add 7L of water to make a slurry, pass the slurry once through a colloid mill, and once through a high-pressure homogenization. Heat to 90°C for 120min, then cool down to room temperature. Ultrasonic treatment, the treatment conditions are 3000W, 60min. Adjust the pH value to 7.0 with 10 mol / L KOH solution, and add 10,000 international units (specific activity) of protease for enzymolysis at 45°C for 240 minutes. After the enzymatic hydrolysis with neutral protease, adjust the pH value to 7.0 with 10 mol / L KOH solution, heat up to 80°C, and add 10,000 international units (specific activity) of amylase for enzymatic hydrolysis for 120 minutes. After enzymatic hydrolysis, heat to 95°C for 10 minutes, then cool down to room temperature. Centrifuge and filter to obtain 3.6L of filtrate and filter residue. Take 3.6L of the filtrate and add 95% ethanol to a final concentration of 75%, let stand overnight, centri...

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Abstract

The invention discloses a preparation method of bitter gourd dietary fibers, which comprises the following steps: (1) washing fresh bitter gourd, cutting the bitter gourd into segments, adding water of which the amount is 4-10 times of the bitter gourd, and pulping; (2) processing the slurry obtained in the step (1) with a colloid mill, and homogenizing at high pressure; (3) heating the slurry to80-100 DEG C and keeping for more than 30 minutes, and reducing the temperature to room temperature; (4) performing ultrasonic treatment on the slurry obtained in the step (3), adjusting the pH valueto 6.5-7.5, and performing enzymolysis by adding protease with specific activity of 1,000-20,000 international units into each kilogram of bitter gourd; (5) after the enzymolysis, adding amylase withspecific activity of 1,000-20,000 international units into each kilogram of bitter gourd; after the enzymolysis is finished, performing enzyme deactivation; and reducing temperature to room temperature; (6) filtering the enzymolysis liquid obtained in the step (5) to obtain a filtrate and filter residue; adding ethanol with the concentration of no less than 95% into the filtrate until the concentration is 60-85%; standing overnight; and centrifuging and filtering to obtain a precipitate; and (7) combining the filter residue and precipitate obtained in the step (6) to obtain the bitter gourd dietary fiber. Through the invention, the purity of the bitter gourd dietary fibers is over 95%, and the bitter gourd dietary fibers have a good effect in regulating blood sugar.

Description

technical field [0001] The invention relates to the technical field of food and health product additives, in particular to a preparation method and application of bitter gourd dietary fiber. Background technique [0002] Dietary fiber is a carbohydrate that cannot be digested by the human body. It is the sum of edible plant ingredients, carbohydrates and similar substances that can resist digestion and absorption in the small intestine of the human body and can be partially or completely fermented in the large intestine of the human body. Including polysaccharides, oligosaccharides, lignin and related plant substances; lack of dietary fiber in daily diet is the direct or One of the indirect reasons, so dietary fiber is very important to maintain human health. [0003] At present, there are relatively few reports about the dietary fiber of bitter gourd, and it is reported in CN1826976A that a compound enzyme is used: composed of papain and amylase, to extract dietary fiber o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L1/212A23L1/308A23L19/00
Inventor 韩金光吴家强伍曾利
Owner 三沙南海美源岛生物科技有限公司
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