Method for extracting oleanolic acid from chaenomeles fruit and preparing oleanolic acid standard

A technology of oleanolic acid and papaya, applied in the direction of steroids, organic chemistry, etc., can solve the problems of low purity of oleanolic acid and cumbersome extraction process steps, achieve simple and rigorous extraction and separation process, and promote industrial The effect of development and resource abundance

Inactive Publication Date: 2011-11-02
ANHUI AGRICULTURAL UNIVERSITY
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Problems solved by technology

In the study of oleanolic acid in Xuan papaya, there is only one article about the determination of oleanolic acid content. There is an extraction process, but it is mainly a step required in the determination process, not extraction and preparation of products. Therefore, there are essential differences, and The extraction process steps are cumbersome, and the product obtained - oleanolic acid is not high in purity

Method used

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Experimental program
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Embodiment Construction

[0024] Now take the laboratory implementation as an example, and the non-limiting examples are described as follows:

[0025] After screening, cleaning, slicing and drying the Xuan papaya fruit, vacuum-dry at 50°C, pulverize, and pass through a 40-mesh sieve (aperture 0.425mm); add 60-85% ethanol equivalent to 10-15 times the weight of the raw material powder to the raw material powder , 30°C-50°C, 300W power under ultrasonic-assisted extraction for 30-50min, after two extractions, the extract was concentrated in vacuo to recover the solvent to obtain a crude extract. The obtained extract was miscible with water and then extracted three times with an equivalent amount of n-butanol saturated aqueous solution. The organic layers were combined and then concentrated in vacuo to recover n-butanol. The sample solution is obtained by filtering with a microporous membrane of μm. After the sample solution is concentrated, dissolve it with 95% ethanol, load the sample at a flow rate of...

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PUM

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Abstract

The invention discloses a method for extracting oleanolic acid from chaenomeles fruit and preparing an oleanolic acid standard. The method comprises the following steps of: cleaning the chaenomeles fruit, drying the chaenomeles fruit in the shade, crushing the chaenomeles fruit into powder, dissolving the powder by using ethanol, performing ultrasonic aided extraction to obtain a crude extract, adding the crude extract into water for dissolving, adding saturated aqueous solution of n-butanol, performing extraction for many times, concentrating the extract to obtain an extract substance, dissolving the extract substance, performing nano-filtration to obtain sample solution of the oleanolic acid, and performing preliminary purification of macroporous resin, silica gel column chromatography,high performance liquid chromatography (HPLC), chromatographic separation and refining on the obtained sample solution to obtain the oleanolic acid standard. The method is simple and convenient in operation, and the oleanolic acid has high yield and high purity and can be made into the standard.

Description

technical background [0001] The invention belongs to the field of separation and purification of natural products, and in particular relates to a method for extracting oleanolic acid from papaya fruit and preparing a standard product of oleanolic acid. Background technique [0002] Xuan papaya is a famous specialty in Anhui, known as the "fruit of all benefits", and is one of the 30 varieties of both medicine and food announced by the Ministry of Health in 2003. Xuan papaya is rich in flavonoids, saponins, amino acids, superoxide dismutase (SOD) and other biologically active substances, as well as various trace elements such as potassium, calcium, magnesium, zinc, iron, etc. There are broad application prospects in fields such as pharmacy, medical care and green food. [0003] Oleanolic acid (oleanolic acid, OA), also known as tuanggui acid, qingsisu, etc., is a pentacyclic triterpene acid, which belongs to oleanolic acid, and is the main physiologically active substance in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J63/00
Inventor 丁之恩屈晓清盛侠王晓燕
Owner ANHUI AGRICULTURAL UNIVERSITY
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