Enzymolysis method for starchy material and method for preparing citric acid

A starch raw material and amylase technology, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of low yield of liquefied clear liquid, high viscosity of filter residue, and high consumption of sugar-making grains, so as to reduce the Residual starch content, the effect of reducing grain consumption and cost, and reducing grain consumption for sugar production

Inactive Publication Date: 2011-11-09
COFCO BIOTECHNOLOGY CO LTD
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Problems solved by technology

[0004] This process has the following defects: (1) gelatinization is not complete: after many starchy raw material granules with larger particle sizes absorb water and swell, they are only shown to be hydrolyzed by starch on the surface; Granules, only the amorphous region of starch and the crystalline region on the surface swell, and the inner crystalline region is difficult to achieve gelatinization
(2) Incomplete liquefaction: As we all know, it is difficult for amylase to interact with unswollen starch crystal regions. Therefore, although more amylase has been added, the starchy raw material is still difficult to liquefy, even if the liquefied liquid is finally tried with iodine. Qualified, but liquefied hydraulic filtration is difficult, the filter residue is viscous and has high water content, which not only brings part of the sugar liquid into the filter residue, but also causes waste of amylase
(3) High residual starch: the ungelatinized crystalline starch is finally trapped in the filter residue during the press filtration process, resulting in high residual starch detected by the enzymatic method of the filter residue
(4) High turbidity of liquefied supernatant: due to poor protein flocculation, it is difficult to press filter, resultin

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  • Enzymolysis method for starchy material and method for preparing citric acid

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[0031] The present invention also provides a method for preparing citric acid. The method includes inoculating Aspergillus niger into a fermentation broth and fermenting to produce citric acid. The fermentation broth contains an enzymatic hydrolysis product of a starch material, characterized in that the starch The preparation method of the enzymatic hydrolysis product of the raw material is the above-mentioned enzymatic hydrolysis method.

[0032] According to the present invention, the content of each component in the fermentation broth can be changed within a wide range, and preferably, a supplementary nitrogen source can be added to the fermentation broth as needed, and the content of the supplementary nitrogen source can be 0.05-0.2% by weight of the total weight of the fermentation broth. According to the present invention, the type of the supplementary nitrogen source is well known to those skilled in the art. For example, the supplementary nitrogen source may be at least ...

Embodiment 1

[0053] This example is used to illustrate the enzymatic hydrolysis method of corn raw material and the method of preparing citric acid of the present invention.

[0054] (1) Crushing of corn raw materials

[0055] 100 parts by weight of corn (moisture content of 14% by weight) were pulverized to obtain a pulverized product with an average particle diameter of 400 microns, and the pulverized product was mixed with 300 parts by weight of water to obtain a starch slurry.

[0056] (2) Enzymatic hydrolysis

[0057] At 50 ℃, the starch slurry obtained in step (1) (the pH value is adjusted to 6.0) is mixed with 30% by weight of the total weight of the amylase (the total amount of amylase is based on the crushed per gram Based on the dry weight of the product, add 30 enzyme activity units of high-temperature resistant α-amylase) to obtain a mixture, and spray the mixture with steam at 170°C in an ejector (the weight ratio of steam to mixture is 0.05:1) , The contact time is 2 seconds, so tha...

Embodiment 2

[0074] This example is used to illustrate the enzymatic hydrolysis method of corn raw material and the method of preparing citric acid of the present invention.

[0075] (1) Crushing of corn raw materials

[0076] 100 parts by weight of the same corn raw material as in Example 1 was pulverized to obtain a pulverized product with an average particle diameter of 600 microns, and the pulverized product was mixed with 300 parts by weight of water to obtain a starch slurry.

[0077] (2) Enzymatic hydrolysis

[0078] At 60°C, the starch slurry obtained in step (1) (pH adjusted to 7.0) is mixed with 40% by weight of the total weight of amylase enzyme (the total amount of amylase is based on the amount of crushed per gram Based on the dry weight of the product, add 20 enzyme activity units (high temperature resistant α-amylase) to obtain a mixture, and spray the mixture with steam at 160°C in an ejector (the weight ratio of steam to mixture is 0.06:1) , The contact time is 3 seconds, so that...

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Abstract

The invention provides an enzymolysis method for a starchy material, which is characterized by comprising the following steps of: (1) mixing starch slurry prepared from the starchy material and partial amylase to obtain a mixture, contacting the mixture with steam, ensuring that the mixture contacted with the steam has the temperature of 100 to 120DEG C, and keeping at the temperature for 4 to 10 minutes; and (2) flashing the mixture contacted with the steam in the step (1), cooling to the temperature of between 85 and 99DEG C, and mixing with the rest amylase for enzymolysis. By the method, the protein flocculation effect can be improved, so that solid and liquid are easy to separate, the content of residual starch in enzymolysis residue is reduced, and the dextrose equivalent (DE) value of enzymolysis clear liquid is effectively improved; in addition, the sugaring yield is improved, the sugaring grain consumption is reduced, and the grain consumption and cost for producing citric acid are reduced.

Description

Technical field [0001] The invention relates to an enzymatic hydrolysis method of starch material and a preparation method of citric acid. Background technique [0002] Citric acid is an organic acid widely used in beverage, food and medicine industries. At present, citric acid is mainly prepared by fermentation. For example, it is generally necessary to pulverize the starch material first, mix the pulverized product with water to obtain a starch slurry, and mix the starch slurry with enzymes for enzymatic hydrolysis to obtain an enzymatic hydrolysis product (enzymatic hydrolysis). Liquefaction solution), and inoculate Aspergillus niger into the fermentation broth containing the enzymatic hydrolysis product to ferment to produce citric acid. [0003] In the above process, the enzymatic hydrolysis (liquefaction) of starch raw materials is very important. The current enzymatic hydrolysis (liquefaction) process of starch raw materials mostly uses a secondary jet liquefaction process...

Claims

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Application Information

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IPC IPC(8): C12P19/14C12P7/48C12R1/685
Inventor 张军华章辉平朱继成卢宗梅杨儒文
Owner COFCO BIOTECHNOLOGY CO LTD
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