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Inhalant comprising modified superoxide dismutase

A superoxide and inhalant technology, which is applied in the directions of medical preparations containing active ingredients, medical preparations without active ingredients, and drug combinations, etc. Excellent stability and high performance

Inactive Publication Date: 2011-11-09
LTT BIO PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0016] So far, the idea of ​​administering PC-SOD itself directly to the lung tissue through the respiratory tract to treat interstitial pneumonia, especially idiopathic interstitial pneumonia, has not concretely existed

Method used

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  • Inhalant comprising modified superoxide dismutase
  • Inhalant comprising modified superoxide dismutase
  • Inhalant comprising modified superoxide dismutase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0092] Example 1: Effect of PC-SOD on bleomycin-induced lung injury model mice

[0093] Bleomycin 5 mg / kg was administered to ICR mice (6-8 weeks old, body weight: 28-32 g) through the respiratory tract to cause lung injury.

[0094] To confirm the formation of lung damage, measure macrophages, lymphocytes, and neutrophils in the alveolar washing fluid to confirm the state of cell infiltration (inflammation state), and measure the amount of hydroxyproline (collagen) to confirm the fiber status.

[0095] Taking the lung injury model mice as objects, PC-SOD of various concentrations were administered once a day through various administration routes of intravenous administration (intravenous), respiratory tract administration (intratracheal) and pulmonary inhalation administration (inhalation). medication.

[0096] The mice were euthanized 6 hours after the last administration, the serum and lung tissue were collected, and the concentrations of PC-SOD in the serum and in the ...

Embodiment 2

[0102] Embodiment 2: The effect of PC-SOD via respiratory tract administration on bleomycin-induced lung injury (acute) model mice

[0103] Bleomycin 5 mg / kg was administered to ICR mice (6-8 weeks old, body weight: 28-32 g) through the respiratory tract to cause lung injury. Confirmation of lung injury formation was the same as in Example 1.

[0104] Using these lung injury model mice as objects, PC-SOD was dissolved in 5% xylose at concentrations of 0.15kU / kg, 0.75kU / kg, 1.5kU / kg, 3.0kU / kg, 15kU / kg and 30kU / kg Alcohol was administered intratracheal once a day for 3 days at a dose of 15 μL to each mouse.

[0105] After 3 days, the alveolar washing fluid was recovered from the mice, and the total cell number was counted. Then, each cell of alveolar macrophages, lymphocytes, and neutrophils was stained by the Diff-Quik method, and the number of the cells was counted.

[0106] figure 1 gives the total number of cells in the alveolar washing fluid, figure 2 Given the resu...

Embodiment 3

[0109] Embodiment 3: The effect of PC-SOD via respiratory tract administration on bleomycin-induced lung injury (chronic) model mice

[0110] Bleomycin 5 mg / kg was administered to ICR mice (6-8 weeks old, body weight: 28-32 g) through the respiratory tract to cause lung injury. Confirmation of lung injury formation was the same as in Example 1.

[0111] Taking the lung injury model mice as objects, PC-SOD was dissolved in 5% xylitol at concentrations of 0.15kU / kg, 0.75kU / kg, 1.5kU / kg and 15kU / kg, and 15 μL of each mouse Dosage Once a day through the respiratory tract (intratracheal) for 14 days.

[0112] On the 15th day, mice were euthanized, slices of lung tissue were made, H&E staining and Masson's trichrome staining were performed to observe changes in lung tissue, and the amount of hydroxyproline (collagen) in the lung tissue was quantified and observed. Fibrotic status of the lungs.

[0113] In addition, as a control, an example in which administration was performed ...

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Abstract

Disclosed is an inhalant particularly for treating idiopathic (acute) or chronic interstitial pneumonia, which comprises, as an active ingredient, a lecithin-bound superoxide dismutase (PC-SOD) that can exert the activity of a superoxide dismutase (SOD) efficiently. Specifically disclosed is an inhalant characterized by comprising, as an active ingredient, a PC-SOD represented by general formula (I): SOD'(Q-B)m (I) (In the formula, SOD' represents a residue of a superoxide dismutase; Q represents a chemical cross-linkage; B represents a residue produced by removing an hydrogen atom from a hydroxy group in lysolecithin having the hydroxy group at position-2 in a glycerol moiety thereof; and m represents an average number of lysolecithin molecules bound to one superoxide dismutase molecule and is an integer of 1 or greater.).

Description

technical field [0001] The present invention relates to an inhalant containing superoxide dismutase. Specifically, the present invention relates to an inhalant containing lecithinated superoxide dismutase (hereinafter sometimes abbreviated as PC-SOD) as an active ingredient, especially for treating interstitial Inhalants for pulmonary diseases such as pneumonia. Background technique [0002] Superoxide dismutase (hereinafter sometimes abbreviated as SOD) is a physiologically active protein extracted from the blood of cattle by Huber et al. in 1965 as an anti-inflammatory protein. It has been clarified that it can specifically remove Peroxide anion (O 2 - ). In organisms, reactive oxygen species are mainly released from phagocytes such as neutrophils and macrophages for sterilization, and usually relative to excess reactive oxygen species, there are various antioxidant substances headed by SOD to defend against reactive oxygen species Damage to normal cells. [0003] How...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/44A61K9/10A61K9/14A61K9/72A61K47/26A61K47/48A61P11/00
CPCA61K9/0078A61K47/10A61K38/446A61K47/186A61K47/48053A61K9/0075A61K47/544A61K47/26A61P11/00A61P31/00A61K9/10A61K9/14
Inventor 水岛徹
Owner LTT BIO PHARMA
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