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A kind of silk fibroin tubular scaffold and preparation method thereof

A silk fibroin and tubular technology, applied in the field of silk fibroin tubular scaffolds and its preparation, can solve the problems of limited application space and low mechanical properties of silk fibroin scaffold materials, and achieve good tissue compatibility and excellent biocompatibility Effects on sexual and mechanical properties, good cytocompatibility and degradation properties

Inactive Publication Date: 2011-12-14
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Another example: the silk fibroin single-layer tubular material was prepared by electrospinning (International Journal of Biological Macromolecules 2009 (45): 504-510), but the mechanical properties of the silk fibroin scaffold material prepared according to the above method were all low. This also limits its application space

Method used

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  • A kind of silk fibroin tubular scaffold and preparation method thereof
  • A kind of silk fibroin tubular scaffold and preparation method thereof

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preparation example Construction

[0029] The embodiment of the invention discloses a method for preparing a silk fibroin tubular scaffold, comprising:

[0030] Weaving the boiled silk thread of silkworm into tubular braid;

[0031] degumming, dialysis and purification of silkworm raw silk to obtain silk fibroin solution;

[0032] Put the tubular braid, silk fibroin solution and adhesive into the mold, take out the tubular mold after being frozen at -80°C to -20°C, and obtain an outer layer of silk fibroin film, a core layer of braid and an inner layer in sequence. Silk fibroin tubular scaffold for silk fibroin membrane.

[0033] The silk fibroin tubular stent prepared as above includes sequentially arranged: an outer layer, a core layer and an inner layer, the outer layer and the inner layer are silk fibroin membranes, and the core layer is a braided silkworm silk thread. Among them, the core layer is the skeleton of the entire tubular stent, and the cooked silk thread is a thread formed by twisting several ...

Embodiment 1

[0047] 1. Weave 8×(20 / 22) boiled silk threads into a small-diameter tubular braid with an inner diameter of 3mm.

[0048] 2. Put 0.2 kg silkworm raw silk into 10 liters of sodium carbonate aqueous solution with a concentration of 0.25 g / L, and treat it three times at 98-100° C., and use tap water for the first two times, each time for 30 minutes. For the third time, use deionized water for 45 minutes. Then the silk is fully washed with water, loosened, and dried in an oven at 60° C. to obtain degummed silkworm silk fiber.

[0049] 3. Weigh 10 grams of degummed silkworm silk fibroin and dissolve it in 100 ml of calcium chloride-ethanol aqueous solution with a molar ratio of 1:2, and dissolve at 70° C. for 2 hours to obtain silkworm silk fibroin solution.

[0050] 4. Pour the silkworm fibroin solution into the dialysis bag. The wall of the dialysis bag is a semi-permeable membrane with a molecular weight cut-off of 3.0-20.0kDa. Place the dialysis bag filled with the silkworm si...

Embodiment 2

[0056] 1. Weave 8×(20 / 22) boiled silk threads into a small-diameter tubular braid with an inner diameter of 3mm.

[0057] 2. Put 0.2 kg of silkworm silkworm silk into 10 liters of sodium carbonate aqueous solution with a concentration of 0.25 g / L, and treat it three times at 98-100 ° C. The water in the aqueous sodium carbonate solution used for the first two times is tap water, and each treatment is 30 minutes, and the water in the aqueous sodium carbonate solution used for the third time is deionized water, and the treatment time is 45 minutes. Then the silk is fully cleaned with deionized water, loosened, and dried in an oven at 60° C. to obtain degummed silkworm silk fiber.

[0058] 3. Weigh 10 grams of degummed silkworm silk fibroin and dissolve it in 100 ml of calcium chloride-ethanol aqueous solution with a molar ratio of 1:2, and dissolve at 70° C. for 2 hours to obtain silkworm silk fibroin solution.

[0059] 4. Pour the silkworm fibroin solution into the dialysis ba...

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Abstract

The invention provides a silk fibroin tubular support and a preparation method thereof. The tubular stent includes an outer layer, a core layer and an inner layer arranged in sequence, the outer layer and the inner layer are silk fibroin films, and the silk fibroin films include cross-linked silk fibroin and an adhesive; the core layer is Bombyx mori cooked silk thread tubular braid. The bending inner diameter of the tubular stent provided by the present invention is 5mm-7mm. The tubular scaffold provided by the invention has no cytotoxicity and has excellent biomechanical properties.

Description

technical field [0001] The invention relates to the field of biological materials, in particular to a silk fibroin tubular support and a preparation method thereof. Background technique [0002] Artificial nerve guides, artificial bile ducts, artificial blood vessels, artificial kidney ducts and other grafts have become urgently needed clinical materials. Taking artificial blood vessels as an example, cardiovascular disease has become the number one killer of human beings, seriously threatening human health. In recent years, with the rapid development of vascular surgery technology and bio-stent materials, tubular stent materials such as artificial blood vessels play an irreplaceable role in vascular surgery, and are currently the most urgently needed biomedical materials and clinical materials. [0003] At present, the artificial blood vessel products used in medicine are mainly made of polyester, polyurethane fiber and expanded polytetrafluoroethylene materials. The above...

Claims

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Application Information

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IPC IPC(8): A61F2/06A61F2/04A61L27/14
Inventor 王建南刘志武
Owner SUZHOU UNIV
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