Kit signal-related gene qPCR chip

A signal-related and gene-based technology, applied in the biological field, can solve problems such as high throughput, high price, and poor pertinence of gene chips, and achieve the effects of poor pertinence, low cost, and simple operation and data analysis

Inactive Publication Date: 2011-12-28
HANGZHOU NORMAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0006] The present invention provides a KIT signal-related gene QPCR chip aimed at the defects of the prior art and the actual needs of KIT signal research. The KIT signal-related gene QPCR chip can be used to detect changes in the expression abundance of KIT signal-related genes. Modification of individual genes according to the requirements of the signal pathway has certain flexibility, which solves the technical defects of poor pertinence, high throughput and high price of existing gene chips

Method used

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  • Kit signal-related gene qPCR chip
  • Kit signal-related gene qPCR chip
  • Kit signal-related gene qPCR chip

Examples

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Embodiment Construction

[0019] The following are specific examples of the present invention; these examples can further supplement and illustrate the present invention; but the present invention is not limited to these examples.

[0020]The techniques used in the following examples, unless otherwise specified, are conventional techniques known to those skilled in the art; the instruments and equipment, reagents, etc. used, unless otherwise specified in this specification, are all research and techniques in this field Personnel are available through public means.

[0021] 1. Experimental materials

[0022] a. Experimental animals and feeding environment

[0023] The B used in this experiment 6 Mice are clean grade, provided by the Experimental Animal Center of Hangzhou Normal University, experimental animal production license: SCXK (Zhejiang) 2005-0020, use license: SCXK (Zhejiang) 2005-0071.

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Abstract

The invention belongs to the field of biotechnology, and relates to a QPCR chip for detecting KIT signal-related genes. A KIT signal-related gene QPCR chip, characterized in that: the chip uses Akt1, Bcl2, Cbl, Ccnd3, Ccnd3-ps, Csf1, Crk, Ecm1, Fes, Fyn, Grb7, Hras1, Jak1, Kitl, Lyn, Map2k , Matk, Myb, Nr0b2, Ntrk1, Pdgfa, Pik3ca, Ppp1r13b, Prkcc, Raf1, Sh2b2, Shc1, Snai2, Soat1, Stat1, Zhx2 genes were used as detection sites, and Actb was used as internal reference. The present invention provides a KIT signal-related gene QPCR chip aimed at the defects of the prior art and the actual needs of KIT signal research. The KIT signal-related gene QPCR chip can be used to detect changes in the expression abundance of KIT signal-related genes. Modification of individual genes is required by the signaling pathway, which has certain flexibility and solves the technical defects of poor pertinence, high throughput and high price of existing gene chips.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a QPCR chip for detecting KIT signal-related genes. Background technique [0002] The human KIT proto-oncogene originated from HZ4 feline sarcoma virus, located on human chromosome 4q12, in the region around the centrosome of the long arm of chromosome 4, and is an allele of the white spot dominant gene. In mice, the gene is located at about 42 cM from the centromere on chromosome 5. The full length of human KIT genomic DNA is about 89kb, including 21 exons. The protein encoded by the KIT gene is called KIT or c-kit receptor, which is a type I transmembrane glycoprotein with a molecular weight of 145kD. It belongs to the type III protein tyrosine kinase receptor superfamily member and is distributed on the cell membrane surface. Its structure is similar to macrophage colony-stimulating factor and platelet-derived growth factor receptor, and consists of three parts: extracellular doma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C40B40/06
Inventor 吴宝金
Owner HANGZHOU NORMAL UNIVERSITY
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