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Cyromazine and melamine residue dual detection rapid detection test strip and preparation method thereof

A technology of melamine and cyromazine, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of long analysis time, high detection cost, complicated sample pretreatment, etc., and achieve low false positive, sensitive detection, and high detection of samples wide range of effects

Inactive Publication Date: 2011-12-28
CHONGQING ACADEMY OF SCI & TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Instrument testing mainly has defects such as expensive instrument purchase, complicated sample pretreatment, cumbersome and time-consuming procedures, high testing costs, and inability to operate on-site, so its application in production is limited
The ELISA method has high sensitivity and low cost, and is widely used in the detection of drug residues. However, this method requires laboratory equipment such as a microplate reader, and the analysis time is long, which has limitations in application.
At present, there is no report on the immunocolloidal gold test strip and its preparation method for the multi-residue dual detection of cyromazine and melamine

Method used

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  • Cyromazine and melamine residue dual detection rapid detection test strip and preparation method thereof
  • Cyromazine and melamine residue dual detection rapid detection test strip and preparation method thereof
  • Cyromazine and melamine residue dual detection rapid detection test strip and preparation method thereof

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Embodiment

[0028] 1 Preparation of drug (CA or MA)-carrier protein conjugates

[0029] The immunogen and the coating agent (CA-BSA, MA-BSA) synthesis method on the test strip detection line of the present invention. Accurately weigh BSA (0.001mmol), and dissolve it in 10mL of PBS at room temperature. Take CA (or MA) standard (0.02 mmol), dissolve it in 1ml of methanol at room temperature, and slowly add 25% glutaraldehyde solution and CA (or MA) solution to the BSA solution at room temperature under a magnetic stirrer. React for 3 h. The ligation product was put into a dialysis bag, dialyzed with normal saline for three days, freeze-dried, and stored at -20°C for future use.

[0030] 2 Preparation of anti-CA and MA monoclonal antibodies

[0031] 2.1 Animal immunization

[0032]The immunogens (CA-BSA, MA-BSA) prepared in Example 1 were used to immunize 6-week-old female Balb / c mice respectively, and the immunization dose of each mouse was 100 μg / 0.2 mL. For the first immunization, di...

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Abstract

The invention discloses a rapid detection test strip for combined detection of cyromazine and melamine residues and a preparation method thereof. The nitrocellulose membrane (4) is coated with a rabbit anti-mouse IgG quality control line (5) and MA-BSA, CA - two detection lines for BSA (6); the anti-MA drug monoclonal antibody-colloidal gold marker and the anti-CA drug monoclonal antibody-colloidal gold marker coated on the binding pad (8). The present invention screens out anti-MA drug monoclonal antibodies and anti-CA drug monoclonal antibodies by using immunological technology, and through the preparation of colloidal gold and the exploration and research of colloidal gold labeling conditions, in the application of anti-MA drug monoclonal antibodies, anti-CA drug monoclonal antibodies Research on Cloning Antibody and Colloidal Gold Technology Establish colloidal gold test strips for dual detection of anti-MA and CA drugs, and measure their performance. The test strip has a wide range of detection samples, low false positives, sensitive, accurate and reliable detection, and is suitable for dual detection of CA and MA drug residues in animal edible tissues such as muscle, liver and kidney and milk.

Description

technical field [0001] The invention belongs to the technical field of immunological rapid detection of food drug residues. Specifically, it relates to a rapid detection test strip of double colloidal gold immunochromatography for residues of cyromazine and melamine and a preparation method thereof. Background technique [0002] Cyromazine (CA) is a highly effective insect growth regulator. In the aquaculture industry, it is mainly used as a premix to kill fly maggots through feed. The main function of cyromazine is to control flies and maggots in animal stables. Cyromazine is mixed into the full-price feed as a premix and enters the animal body through feeding. Most of it (about 99%) is excreted with feces in the form of the original form and its metabolite (melamine) and deposited in the in feces, thereby killing fly maggots in feces. The acute and chronic toxicity of cyromazine is very low, and it has no teratogenic, carcinogenic, or mutagenic effects, and its main me...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/532
Inventor 乐涛魏启明
Owner CHONGQING ACADEMY OF SCI & TECH
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