Applications of beta-lactamase in improving functional protein folding and preparation of fusion protein

A technology of lactamase and fusion protein, applied in the field of fusion protein, can solve the problem that the leader sequence does not have a catalytic effect, and achieve the effect of less steps and promoting folding
CN102321602AActive Publication Date: 2012-01-18TONGHUA DONGBAO PHARMA
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Patent Information

Authority / Receiving Office
CN Β· China
Current Assignee / Owner
TONGHUA DONGBAO PHARMA
Publication Date
2012-01-18

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Abstract

The invention provides applications of beta-lactamase or peptide of which the identity is at least 20% with a beta-lactamase amino acid sequence as leading peptide in improving functional protein folding. One or more of resectable amino acid residues in a leader sequence used in the invention are same with one or more resectable amino acid residues of an isolated leader sequence and a to-be-folded protein precursor, which is good for resecting leader sequence segments after folding, thus the purification steps at the down stream can be simplified, and also the leader protein provided by the invention can improve the folding of functional protein.
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Description

technical field

[0001] The present invention relates to a new application of Ξ²-lactamase, in particular to the new application of Ξ²-lactamase as a leading protein to promote the folding of functional protein, and the fusion protein prepared from the leading protein. Background of the invention

[0002] The leader sequence of many proteins is essential for their correct folding and maturation. This leader sequence-dependent protein folding phenomenon was first discovered in 1987 by Ikemura et al. when studying subtilisin. Subtilisin is a kind of alkaline serine protease from Bacillus subtilis, the N-terminus of its zymogen is a leader sequence consisting of 77 amino acid residues. It is well documented that protein folding is regulated by a leader sequence in subtilisins. The "subtilisins" family includes enzymes of prokaryotic, eukaryotic and arcuate origin. The leader sequence is important for the production of active subtilisins both in vivo and in vitro, and as a leader...

Claims

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