Control gene of rice panicle pedicel length and application thereof

A technology of spike and stem node and gene, applied in the field of rice spike stem node length control gene CSP1

Inactive Publication Date: 2012-01-18
FUJIAN AGRI & FORESTRY UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Several rice panicle mutants shp1 / 2 / 5 / 6 (sheathed panicle) and fsp (fully sheathed panicle) have been discovered. There are still very few studies on the rice panicle internode elongation, and the root cause of rice spike neck elongation has not been deeply studied (Kinoshita, 1986; Zhu Keming, 2006; Liu Zhuang et al., 2006; Wang Weiping, 2008)

Method used

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  • Control gene of rice panicle pedicel length and application thereof
  • Control gene of rice panicle pedicel length and application thereof
  • Control gene of rice panicle pedicel length and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1: rice panicle stem node length control gene CSP1 Bitcloning of

[0036] 1. Rice material:

[0037] Rice ( Oryza sativa L) Mutant csp1 , the original wild-type material is Minghui 86. The mutant was obtained by the present inventors from the offspring of Minghui 86 immature embryo tissue culture regenerated plants.

[0038] 2. Analyze and target groups:

[0039] mutant csp1 Crossed with the wild-type variety Xiushui 13, F 1 F2 populations were obtained by selfing in the first generation, and 2720 individuals were selected at the heading stage csp1 Plants with mutant phenotypes were used as the targeting population. At the heading stage, about 1 gram of leaves were taken from each plant to extract the total DNA.

[0040] 3. Mapping using rice microsatellite markers (RM and SSR) and InDel markers CSP1 Gene:

[0041] The total DNA of rice was rapidly extracted by the rice micro-method. Take about 0.3 grams of rice leaves, put them into a 1...

Embodiment 2

[0048] Example 2 Genetic Transformation

[0049] According to the sequence of the indica rice Minghui 86 gene, the pCAMBIA1301 vector was used to construct the following Figure 4 shown CSP1 Candidate Gene Complementation Experiment Vector pCAMBIA1301- CSP1 . The specific process of vector construction is: design a pair of primers with restriction sites in the candidate phosphatidylserine synthetase gene region and promoter region, use high-fidelity enzymes to perform high-fidelity PCR amplification and sequencing, and select the sequence is completely correct The clones were digested and ligated, and constructed as Figure 4 The indicated expression vector was then transformed into Agrobacterium. The DNA sequence of the complementary vector is 7334bp, including 1932bp before the start codon and 455bp after the stop codon. The primers used to amplify the DNA sequence of the complementary experiment are (the underlined part is the restriction site and the protection base...

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Abstract

The invention belongs to the technical field of plant genetic engineering, and discloses a control gene CSP1 of rice panicle pedicel (first internode from top) length and applications thereof. The invention particularly relates to positioning, clone, function verification, and applications of the gene for controlling rice panicle pedicel length. The nucleotide sequence of the CSP1 gene is shown in sequence table SEQIDNO. 1, and a coded amino acid sequence is shown in sequence table SEQIDNO. 2. CSP1 has no obvious influence on the nutrition growth of rice or the development of panicles, and mainly controls the growth and development of rice panicle pedicels. The expression level of the gene in rice panicle pedicels is adjusted on purpose by genetic engineering technology, and thus the growth and development of rice panicle pedicels can be regulated and controlled; the panicle enclosure problem of sterile lines during hybrid rice seed production is solved; the yield and quality of hybrid rice seeds are improved; the cost is reduced; and environment pollution caused by the application of plant exogenous hormones is reduced. Therefore, the gene has quite important application value and wide application prospects.

Description

technical field [0001] The invention relates to the field of plant genetic engineering. Specifically, it relates to a rice panicle stem node length control gene cloned by map-based cloning technology CSP1 (Completely Sheathed Panicle 1), and the function of the gene was verified by the transgene complementation experiment. At the same time, it also involves the use of this gene to regulate the growth and development of rice panicle stem nodes, solve the ear wrapping of sterile lines in the process of hybrid rice seed production, improve the yield and quality of hybrid rice, reduce the production cost of hybrid rice, and reduce the risk caused by the application of exogenous plants. Environmental pollution caused by hormones. Background technique [0002] Rice is one of the main food crops in my country, and about 40% of the world's population uses rice as a staple food. Since the 1960s, the world's population has grown rapidly and the area of ​​arable land has been sh...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/55C12N9/16C12N15/84A01H5/00
Inventor 吴为人段远霖官华忠王锋刘华清庄丽君亓文明
Owner FUJIAN AGRI & FORESTRY UNIV
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