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Fermentation method for promoting biosynthesis of antrodia cinnanonea active product of Antrodin C

A technology of biosynthesis and fermentation method, which is applied in the direction of microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve the problems of Antrodins and Antroquinonol, which do not have the characteristic active products of Antrodia camphorata, in the cultivation method and process optimization, so as to increase production, The effect of simple technical process

Active Publication Date: 2012-01-18
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

According to our novelty search report, there are currently no reports on the cultivation method and process optimization of Antrodins and Antroquinonol, the characteristic active products of Antrodia camphorata

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The purpose of this experiment is to investigate the influence of dissolved oxygen concentration and pH value on the growth of Antrodia camphorata cells and the synthesis of active products when the liquid fermentation process is not controlled.

[0022] 1. Antrodia camphorata slant culture, first-level liquid seed culture, and second-level liquid seed culture are carried out according to the following medium and culture conditions:

[0023] The slant culture medium is PDA (potato dextrose agar); the culture conditions of Antrodia camphorata slant strains are: the culture temperature is 28° C., and the culture time is 12 days.

[0024] Described primary liquid seed culture medium is (g / L): glucose 20, Na 2 HPO 4 0.5, MgSO 40.5, citric acid 0.5, soybean powder 4, corn syrup powder 2. The cultivation conditions of the primary liquid seeds are as follows: cultivation temperature 28°C, shaker rotation speed 100r / min, cultivation time 4d.

[0025] Described secondary li...

Embodiment 2

[0029] The purpose of this experiment was to investigate the effect of two-stage control of dissolved oxygen on the growth of Antrodia camphorata cells and the synthesis of active products in the liquid fermentation system.

[0030] In the present embodiment, culture of Antrodia camphorata slant strain, first-level liquid seed culture, second-level liquid seed culture and submerged liquid fermentation are completely the same as in Example 1; in the first stage of fermentation (first 5 days), the dissolved oxygen concentration is controlled at 20 %, in the second stage of fermentation (starting from the 5th day), the dissolved oxygen concentration is controlled at 10%, and the fermentation temperature is controlled to be 28°C. Using this dissolved oxygen two-stage controlled culture Antrodia camphorata, the maximum biomass is 13.7g / L, the maximum content of Antrodin C is 217.3mg / L, and the detection induced Antroquinonol, its content is 13.4mg / L.

Embodiment 3

[0032] The purpose of this experiment was to investigate the effect of two-stage control of dissolved oxygen on the growth of Antrodia camphorata cells and the synthesis of active products in the liquid fermentation system.

[0033] In the present embodiment, culture of Antrodia camphorata slant strain, first-level liquid seed culture, second-level liquid seed culture and submerged liquid fermentation are completely the same as in Example 1; in the first stage of fermentation (first 5 days), the dissolved oxygen concentration is controlled at 20 % or more, in the second stage of fermentation (from the 5th day), the dissolved oxygen concentration is controlled at 6%, and the controlled fermentation temperature is 18°C. Antrodia camphorata was cultivated with this two-stage control of dissolved oxygen, the maximum biomass was 11.2g / L, the maximum Antrodin C content was 173.6mg / L, and the induced Antroquinonol content was 5.3mg / L.

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Abstract

The invention discloses a fermentation method for promoting biosynthesis of an antrodia cinnanonea active product of Antrodin C, which comprises the following steps of slant strain culture, first-order liquid seed culture, second-order liquid seed culture, and liquid submerged fermentation, wherein the liquid submerged fermentation is controlled by two phases; in the first phase, dissolved oxygenis controlled to be above 20%; in the second phase, dissolved oxygen is controlled to be 6-15%; and the fermentation temperature is 18 DEG C-28 DEG C; in addition, a fermentation method with the combination of dissolved oxygen and pH is adopted; with the combined control of the pH and the dissolved oxygen in the two phases, the synthesis of antrodia cinnanonea active product of Antrodin C is significantly promoted with a maximal content of up to 271.8 mg / L. The control technology provided by the invention has a simple process and high efficiency, has significant industrial application value, and has certain enlightening significance on the production of other antrodia cinnanonea active metabolites.

Description

technical field [0001] The invention relates to a fermentation method for promoting the biosynthesis of Antrodia camphorata active product Antrodin C, in particular to a method for improving the biosynthesis of the active product Antrodin C through a two-stage dissolved oxygen or a strategy of combining dissolved oxygen and pH. technical background [0002] Antrodia camphorata (Antrodia camphorata) belongs to the family Polyporaceae and the genus Lepidobacterium, and is a rare medicinal fungus endemic to Taiwan. For a long time, Antrodia camphorata has been used as a traditional medicine for hangover and liver protection, for liver disease, food and drug-induced poisoning symptoms, diarrhea, abdominal pain, high blood pressure, skin itching and tumor diseases. A large number of literature studies have shown that Antrodia camphorata has biological activities such as liver protection, nervous system protection, anti-hypertension and hyperlipidemia, anti-virus, anti-inflammatio...

Claims

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Application Information

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IPC IPC(8): C12P7/46C12P7/26C12R1/645
Inventor 许赣荣夏永军喻学淳
Owner JIANGNAN UNIV
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