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Tissue restoration structure derived from fish scales

A tissue repair and fish scale technology, applied in medical science, prosthesis, etc., can solve problems such as insufficient oxygen permeability and burden on patients' eyes, and achieve the effect of simple treatment steps.

Inactive Publication Date: 2012-01-25
BODY ORGAN BIOMEDICAL CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the above-mentioned polymer materials are not biodegradable materials, so after implanted in the patient's eyes, they will continue to exist in the patient's body. If it continues to be in good condition after being implanted into a living body (such as a human body), it may still cause a burden on the patient's eye itself

Method used

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  • Tissue restoration structure derived from fish scales
  • Tissue restoration structure derived from fish scales
  • Tissue restoration structure derived from fish scales

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Preparation of decellularized fish scales

[0063] After obtaining about 200 grams of fish scales, immediately wash them at least three times with secondary deionized water until they are clean. Then, the cleaned fish scales are subjected to the decellularization step. The decellularization step can use low osmotic pressure to cause cell bursting (hypotonic), detergent (detergent), Triton X-100, sodium lauryl sulfate Sodium (sodium dodecyl sulfate, SDS), protein enzyme inhibitor (protease inhibitor), deoxyribonuclease (DNase) and ribonuclease (RNase) to treat, and then in order to increase porosity and porosity, so reuse Acetic acid treatment of fish scales through the above steps. After the processed material is rinsed at least three times with sterile phosphate buffer solution, it is soaked in phosphate buffer solution, which is decellularized fish scales.

Embodiment 2

[0065] Preparation of decalcified fish scales

[0066] At room temperature, place fish scales in 5% nitric acid solution for 6-16 hours to reduce inorganic components such as calcium phosphate salts inside fish scales, such as hydroxyapatite (HAP), tricalcium phosphate (tricalcium phosphate, TCP)...etc. Then above-mentioned material is soaked in the time of A solution (10% EDTA, 2% nitric acid) at 4 DEG C again for two to three days, during which and continue to change A solution, then with the gradual increase of immersion time, above-mentioned material will be more Further reduce inorganic components such as calcium phosphate salts, such as hydroxyapatite (HAP), tricalcium phosphate (TCP), etc. The material treated by the above steps was soaked in 70% alcohol and stored at 4 °C.

Embodiment 3

[0068] Oxygen Permeability Measurement of Decellularized and Decalcified Fish Scale

[0069] The oxygen permeability (Dk) of the decellularized and decalcified fish scales can be measured using an oxygen permeability meter (Oxygen Permeometer Model 201T; Createch Inc., Albany, CA, USA) at an absolute humidity of 100% and a temperature of 35°C. Measured, the oxygen permeability of the material in the phosphate buffer solution is 48.15×10 -11 (cm2 x ml O 2 ) / (sec x ml x mmHg), wherein the preferred embodiment of the oxygen permeability of the tissue repair structure is 30-130 (cm 2 x ml O 2 ) / (sec x ml x mmHg).

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Abstract

The invention relates to a tissue restoration structure derived from fish scales. The tissue restoration structure comprises a base material which has the characteristics of good biological compatibility and biological decomposability and is formed by the fish scales processed by proper steps, and the special microchannel structure is in favor of cell attachment and proliferation. Before using the tissue restoration structure in an organism (such as a human body), the LAL (limulus amebocyte lysate) test value in the tissue restoration structure must be less than 1000Eu / ml, and preferably less than 200Eu / ml; and if the tissue restoration structure is used as special medicinal equipment, the LAL test value must be less than 50Eu / ml.

Description

technical field [0001] The invention relates to a biological structure, in particular to a tissue repair structure applied to a living body (such as a human body). Background technique [0002] Millions of people around the world suffer from eye diseases, some congenital and some acquired, such as chemical burns...etc. For patients with mild corneal damage, normal vision can still be maintained with drug treatment; however, for patients with severe corneal damage, surgery is often required. The traditional treatment method is to use corneal transplantation to restore the patient's vision, but the limited source of cornea is a problem that needs to be solved. This factor also makes the research and development of corneal substitutes increasing day by day. [0003] Due to the properties of different materials, corneal substitutes are born using different principles. At present, synthetic polymers are the mainstream, combined with the use of core and skirt technology, to try...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36
Inventor 赖弘基林峰辉林建成林尚明张毓忠
Owner BODY ORGAN BIOMEDICAL CORP
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