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Fermentation production method of high-potency colymycin and culture media used by same

A technology of fermentation medium and carrimycin, applied in the direction of fermentation, can solve the problems of affecting yield and titer, reducing short-chain fatty acids, weakening donor metabolism, etc., to achieve the effect of being beneficial to synthesis and promoting synthesis

Active Publication Date: 2014-05-14
SHANGHAI TONGLIAN PHARMA TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because excessive ammonium will inhibit the activity of acyl CoA synthetase and acyl kinase-acyl phosphotransferase, reduce the availability of short-chain fatty acids in the cell, and weaken the donor metabolism of these precursors, thereby affecting the synthesis of carrimycin products. Affect its yield and potency

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0122] A fermentative production method of high-titer carrimycin, comprising the steps of:

[0123] (1) WSJ-195 glycerin tube for the experimental strain;

[0124] (2) Configure 500mL slant medium, 1000mL shake flask seed medium, and 10L fermenter medium. The medium composition is prepared as follows:

[0125] ①. Inclined culture medium (g / L)

[0126] Glucose 10

[0127] starch 20

[0128] soybean meal powder 10

[0129] NaCl 4

[0130] CaCO 3 5

[0131] Agar 20

[0132] The balance is deionized;

[0133] 0.1 MPa, that is, sterilize at 121°C for 30 minutes for later use;

[0134] ②, seed medium (g / L)

[0135] Glucose 10

[0136] Starch 30

[0137] soybean cake powder 20

[0138] NaCl 4

[0139] CaCO 3 5;

[0140] The balance is deionized water;

[0141] 0.1MPa, that is, 121 ° C, sterilized for 30 minutes for standby;

[0142] ③. Fermentation medium (g / L)

[0143] Glucose 20

[0144] Starch 40

[0145] fishmeal 20

[0146...

Embodiment 2

[0217] A fermentative production method of high-titer carrimycin, comprising the steps of:

[0218] (1) The strain used in the experiment is WSJ-195 glycerin tube;

[0219] (2) Configure 500mL slant medium, 1000mL shake flask seed medium, 10L fermenter medium, and culture

[0220] The base composition is formulated as follows:

[0221] ①. Inclined culture medium (g / L)

[0222] Glucose 10

[0223] starch 20

[0224] soybean meal powder 10

[0225] NaCl 4

[0226] CaCO 3 5

[0227] Agar 20

[0228] The balance is deionized;

[0229] Sterilize at 0.1 MPa (121°C) for 30 minutes for later use;

[0230] ②, seed medium (g / L)

[0231] Glucose 10

[0232] Starch 30

[0233] soybean cake powder 20

[0234] NaCl 4

[0235] CaCO 3 5;

[0236] The balance is deionized water;

[0237] 0.1MPa, that is, sterilized at 121°C for 30 minutes for standby;

[0238] ③. Fermentation medium (g / L)

[0239] Glucose 20

[0240] Starch 40

[0241] fishmeal 20 ...

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Abstract

The invention discloses a fermentation production method of high-potency colymycin and culture media used by the same. The culture media comprise a slant culture medium, a seed culture medium, a fermentation culture medium and a fermentation process material supplement culture medium, namely magnesium phosphate. The fermentation production method comprises seven steps, namely the selection of strains, the preparation of the culture media, the slant seed culture, the shake-flask seed culture, the fermentation tank culture, the separation and purification of products and the drying of the products, and the average biological potency of the obtained colymycin fermentation broth is 2100U / mL which is relatively improved by 16.7%.

Description

technical field [0001] The invention relates to a fermentative production method of high-titer carrimycin and a culture medium used therefor. Background technique [0002] Carrimycin (formerly known as Bitespiramycin, Shengjimycin Bitespiramycin) is the fermentation product of genetically engineered bacteria obtained by genetic recombination technology. The isovaleryltransferase gene (ist), thiostrepton The resistance gene (str) was cloned into the thermosensitive Streptomyces / Escherichia coli shuttle plasmid vector pKC1139 (Apramycin resistance), and the genetically engineered strain WSJ-1 was obtained. It belongs to a new type of macrolide antibiotics. Because it is the first at home and abroad, it obtained the Chinese invention patent (patent number: ZL97104440.6) in November 2000, and on the basis of a large number of preclinical trials, it was awarded the "National Drug Administration" issued by the State Drug Administration in July 2001. New Drug Clinical Research Ap...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/62
Inventor 刘新星郝玉有李彩玉武桂华李志生
Owner SHANGHAI TONGLIAN PHARMA TECH