Preparation method of dynamic magnet-coated nano-sized solid-phase open-tubular capillary electric chromatography column

A technology of capillary electrochromatography and stationary phase, which is applied in the field of preparation of dynamic magnetic coating nanometer stationary phase open-tube capillary electrochromatography column, can solve the problems of low column capacity, small column capacity, difficult regeneration, etc. Improved column capacity and smaller nanoparticle size

Inactive Publication Date: 2012-02-01
EAST CHINA UNIV OF SCI & TECH
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Problems solved by technology

But it has the disadvantage of relatively low and small column capacity
At present, although some improvements have been made to the preparation method of open-tube CEC columns to obtain higher phase ratio and higher column efficiency, there are still problems such as cumbersome column preparation process, long cycle, low

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  • Preparation method of dynamic magnet-coated nano-sized solid-phase open-tubular capillary electric chromatography column
  • Preparation method of dynamic magnet-coated nano-sized solid-phase open-tubular capillary electric chromatography column
  • Preparation method of dynamic magnet-coated nano-sized solid-phase open-tubular capillary electric chromatography column

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Embodiment 1

[0034] A method for preparing a dynamic magnetically coated nanometer stationary phase open-tube capillary electrochromatographic column, the specific steps of which are as follows:

[0035] 1. Functionalization of magnetic nanomaterials:

[0036] core-shell Fe 3 o 4 SiO 2 Magnetic nanomaterials react with silylating reagents to introduce specific functional groups on their surface to prepare the stationary phase required for sample separation;

[0037] 2. Preparation of dynamic magnetically coated nano-stationary phase open-tube capillary electrochromatographic column:

[0038] (1) Selection of permanent magnets

[0039] Permanent magnets are used to provide a stable magnetic field;

[0040] The permanent magnets are cuboids, cubes, cylinders, concentric cylinder magnets, etc.;

[0041] (2) Selection of magnetic field direction

[0042] Clamp 23~50 pairs of permanent magnets as described in (1) on the capillary to provide a locally stable magnetic field in the directio...

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Abstract

The invention relates to a preparation method of a dynamic magnet-coated nano-sized solid-phase open-tubular capillary electric chromatography column. According to the invention, under the effect of an external magnetic field, magnetic nano-sized particles are orientedly distributed on the inner wall of the capillary tube, such that a nano-sized particle solid-phase bed layer with a certain thickness is formed. An advantage of core-shell-structured silica coated magnetic nano-sized particles of extremely high specific surface areas and an advantage of the open-tubular capillary electric chromatography column of fast separation are combined, such that the dynamic magnet-coated nano-sized solid-phase open-tubular capillary electric chromatography column is constructed. With the electric chromatography column provided by the invention, column capacity and column efficiency are greatly improved. With a simple action of turning on/off the magnetic field, polluted or irreversibly adsorbed solid phases can be removed, such that the reclaiming of the dynamic magnet-coated nano-sized solid-phase open-tubular capillary electric chromatography column is realized, and the service life of the capillary column is prolonged. The character is especially suitable to be used in separations of complex samples such as irreversibly adsorbed protein with solid phase of no separation capacity.

Description

【Technical field】 [0001] The invention relates to the technical field of chromatography, in particular to a preparation method and device for a dynamic magnetically coated nano-stationary phase open-tube capillary electrochromatographic column. 【Background technique】 [0002] According to different preparation methods, capillary electrochromatographic columns are divided into monolithic columns, open-tube columns, and packed columns. The monolithic column is obtained by in-situ polymerization in the chromatographic column by an organic or inorganic method to obtain a continuous bed stationary phase. The preparation method of this chromatographic column is simple, no plunger is needed, and a variety of functional groups can be introduced by changing the reaction monomer, which has better porosity and permeability. It avoids the disadvantages of low capacity of the open-tubular column and easy generation of air bubbles at the plug of the packed column. However, the monolithi...

Claims

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Application Information

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IPC IPC(8): G01N30/60
Inventor 张凌怡覃飒飒朱亚仙高方园万莉张维冰
Owner EAST CHINA UNIV OF SCI & TECH
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