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Method for extracting raffinose from cotton seed meal

A technology for raffinose and cottonseed meal, applied in chemical instruments and methods, oligosaccharides, sugar derivatives, etc., can solve the problems of large amount of alcohol used, high industrial cost, low recovery rate of raffinose, etc., and reduce energy consumption. And the effect of cost and production cost reduction

Inactive Publication Date: 2012-02-15
UNIV OF SCI & TECH BEIJING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The purpose of the present invention is to provide a new method for extracting raffinose, which can overcome the shortcomings of the existing alcohol extraction method, such as large amount of alcohol used, low recovery rate of raffinose, high industrial cost, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Weigh 100g cottonseed meal (raffinose content is 5.8%), add deionized water at a mass volume ratio of 1:3 (g / mL), extract at 40°C for 1 hour 3 times, precipitate, filter, and centrifuge to obtain crude Raffinose solution. A hollow ultrafiltration fiber membrane (molecular weight cut-off of 10,000 Dalton) was used for ultrafiltration at a flow rate of 6 mL / min to obtain a purified raffinose solution. At 40 °C, activated carbon was used as the adsorbent, and the static adsorption was carried out at a mass volume ratio of 1:10~1:20. Under the condition of 50°C, shake and elute with 50% ethanol solution for 30 minutes, collect the eluate to obtain a recovered raffinose solution. Concentrate the recovered raffinose solution to 1 / 8 of the original volume by distillation under reduced pressure, add 60% ethanol solution at a volume ratio of 1:3, and then add the standard raffinose for crystallization. The addition of the standard raffinose is 0.05g / L , 0°C for 3 days, separat...

Embodiment 2

[0036] Weigh 200g cottonseed meal (raffinose content is 5.6%), add deionized water at a mass volume ratio of 1:4 (g / mL), extract at 60°C for 3 hours once, settle, filter, and centrifuge to obtain crude Raffinose solution. Add 1% chitosan solution whose volume is 1 / 100 of the crude raffinose solution to carry out flocculation, centrifuge to take the supernatant, and obtain a purified raffinose solution. Under the condition of 60°C, an adsorption exchange column filled with non-polar macroporous acrylic adsorption resin was used for adsorption treatment, and the flow rate was about 4 mL / min. At 50°C, the resin adsorption exchange column was eluted with 30% methanol solution at a flow rate of 3 mL / min until there was no raffinose in the eluate, and the eluate was collected to obtain a recovered raffinose solution. Concentrate the recovered raffinose solution to 1 / 9 of the original volume by distillation under reduced pressure, add 80% ethanol solution at a volume ratio of 1:1, a...

Embodiment 3

[0039] Weigh 200g cottonseed meal (raffinose content is 5.6%), add deionized water at a mass volume ratio of 1:5 (g / mL), extract at 60°C for 1.5 hours twice, precipitate, filter, and centrifuge to obtain crude Raffinose solution. A hollow fiber ultrafiltration membrane (molecular weight cut-off of 10,000 Dalton) was used for ultrafiltration at a flow rate of 8 mL / min to obtain a purified raffinose solution. Under the condition of 70°C, the adsorption treatment was carried out by using a silica gel adsorption exchange column filled with C18 bonded, and the flow rate was about 5 mL / min. At 60°C, the resin adsorption exchange column was eluted with 60% ethanol solution at a flow rate of 2.5mL / min until there was no raffinose in the eluate, and the eluate was collected to obtain a recovered raffinose solution. Concentrate the recovered raffinose solution to 1 / 9 of the original volume by distillation under reduced pressure, add 70% ethanol solution according to 2 times the volume ...

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Abstract

The invention belongs to the field of plant extraction, and relates to a method for extracting raffinose from cotton seed meal. The method comprises the following steps of: extracting the cotton seed meal by using water to obtain crude raffinose solution; removing macromolecular substances from the crude raffinose solution by adding a flocculating agent or using an ultrafiltration membrane to obtain primarily purified raffinose solution; adsorbing the raffinose in the primarily purified raffinose solution by using an adsorbent; eluting the raffinose from the adsorbent by using ethanol or methanol, and collecting eluent to obtain recovered raffinose solution; and concentrating the recovered raffinose solution, adding ethanol solution, adding raffinose crystal seeds for crystallizing, and separating and drying crystals to obtain purified raffinose. Compared with the conventional alcohol extraction method, the method has the advantages that the method is clean, low in energy consumption and cost, easy to operate, high in recovery rate and the like.

Description

[0001] technical field [0002] The invention belongs to the field of plant extraction and relates to a method for extracting raffinose from cottonseed meal. [0003] Background technique [0004] Raffinose, also known as raffinose, is an oligosaccharide in the plant kingdom that is second only to sucrose in oligosaccharides. Raffinose has a variety of health functions for the human body. It can significantly proliferate bifidobacteria and significantly improve the intestinal tract. Bacteria, improving constipation and diarrhea patients' defecation, anti-cancer and other physiological functions. On March 9, 2010, the Ministry of Health of my country announced 7 items including cottonseed oligosaccharides as new resource foods (Bao Shu Yun, Guangzhou Chemical Industry, 2011, 3, 20). In addition, raffinose also has certain applications in medicine and microorganisms. It can also synthesize glycolipids as a substitute for high-energy substances and as a stable component of emul...

Claims

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Application Information

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IPC IPC(8): C07H3/06C07H1/08A23K1/14
Inventor 常雁红罗晖瞿海燕
Owner UNIV OF SCI & TECH BEIJING
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