Method for extracting raffinose from cotton seed meal
A technology for raffinose and cottonseed meal, applied in chemical instruments and methods, oligosaccharides, sugar derivatives, etc., can solve the problems of large amount of alcohol used, high industrial cost, low recovery rate of raffinose, etc., and reduce energy consumption. And the effect of cost and production cost reduction
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Embodiment 1
[0033] Weigh 100g cottonseed meal (raffinose content is 5.8%), add deionized water at a mass volume ratio of 1:3 (g / mL), extract at 40°C for 1 hour 3 times, precipitate, filter, and centrifuge to obtain crude Raffinose solution. A hollow ultrafiltration fiber membrane (molecular weight cut-off of 10,000 Dalton) was used for ultrafiltration at a flow rate of 6 mL / min to obtain a purified raffinose solution. At 40 °C, activated carbon was used as the adsorbent, and the static adsorption was carried out at a mass volume ratio of 1:10~1:20. Under the condition of 50°C, shake and elute with 50% ethanol solution for 30 minutes, collect the eluate to obtain a recovered raffinose solution. Concentrate the recovered raffinose solution to 1 / 8 of the original volume by distillation under reduced pressure, add 60% ethanol solution at a volume ratio of 1:3, and then add the standard raffinose for crystallization. The addition of the standard raffinose is 0.05g / L , 0°C for 3 days, separat...
Embodiment 2
[0036] Weigh 200g cottonseed meal (raffinose content is 5.6%), add deionized water at a mass volume ratio of 1:4 (g / mL), extract at 60°C for 3 hours once, settle, filter, and centrifuge to obtain crude Raffinose solution. Add 1% chitosan solution whose volume is 1 / 100 of the crude raffinose solution to carry out flocculation, centrifuge to take the supernatant, and obtain a purified raffinose solution. Under the condition of 60°C, an adsorption exchange column filled with non-polar macroporous acrylic adsorption resin was used for adsorption treatment, and the flow rate was about 4 mL / min. At 50°C, the resin adsorption exchange column was eluted with 30% methanol solution at a flow rate of 3 mL / min until there was no raffinose in the eluate, and the eluate was collected to obtain a recovered raffinose solution. Concentrate the recovered raffinose solution to 1 / 9 of the original volume by distillation under reduced pressure, add 80% ethanol solution at a volume ratio of 1:1, a...
Embodiment 3
[0039] Weigh 200g cottonseed meal (raffinose content is 5.6%), add deionized water at a mass volume ratio of 1:5 (g / mL), extract at 60°C for 1.5 hours twice, precipitate, filter, and centrifuge to obtain crude Raffinose solution. A hollow fiber ultrafiltration membrane (molecular weight cut-off of 10,000 Dalton) was used for ultrafiltration at a flow rate of 8 mL / min to obtain a purified raffinose solution. Under the condition of 70°C, the adsorption treatment was carried out by using a silica gel adsorption exchange column filled with C18 bonded, and the flow rate was about 5 mL / min. At 60°C, the resin adsorption exchange column was eluted with 60% ethanol solution at a flow rate of 2.5mL / min until there was no raffinose in the eluate, and the eluate was collected to obtain a recovered raffinose solution. Concentrate the recovered raffinose solution to 1 / 9 of the original volume by distillation under reduced pressure, add 70% ethanol solution according to 2 times the volume ...
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