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Fluid nutrient medium for promoting growth of halophilic nitrite oxidizing bacteria

A liquid culture medium, nitrifying bacteria technology, applied in the directions of microorganisms, microorganisms, biochemical equipment and methods, etc., can solve the problems of undisclosed salt-tolerant nitrifying bacteria and the like

Inactive Publication Date: 2012-02-15
SHENYANG JIANZHU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is also an undisclosed liquid medium formula for promoting the growth of salt-tolerant nitrifying bacteria

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Add glucose 0.3g, sodium nitrite 0.6, manganese sulfate monohydrate 0g, potassium monohydrogen phosphate 0.6g, sodium carbonate 0.6g, potassium dihydrogen phosphate 0, sodium chloride 20g, magnesium sulfate 2.8g, magnesium chloride 1.8 in 1000ml water in sequence g, calcium chloride 0.8g, sodium bicarbonate 0g, potassium chloride 0.3g, vitamin solution 1ml, trace element solution 1ml, zeolite 60g, pH=8.

[0017] The nitrifying bacteria strains were inserted into the above-mentioned medium according to the inoculum amount of 10%, and the temperature was 30°C, and the culture was shaken at a constant temperature. After 24 hours of inoculation, the turbidity of nitrifying bacteria was significantly improved; however, the turbidity of the same batch was inoculated to the medium without adding glucose, vitamin solution, and zeolite, and the turbidity of the bacteria did not increase, but decreased slightly. The results show that the liquid culture medium provided by the inve...

Embodiment 2

[0019] Add glucose 0.7g, sodium nitrite 1.4g, manganese sulfate monohydrate 0.04g, potassium monohydrogen phosphate 1.5g, sodium carbonate 1.4g, potassium dihydrogen phosphate 0.6g, sodium chloride 32g, magnesium sulfate 3.6g in 1000ml water in turn , magnesium chloride 2.6g, calcium chloride 1.4g, sodium bicarbonate 0.6g, potassium chloride 1.1g, vitamin solution 10ml, trace element solution 10ml, zeolite 80g, pH=8.

[0020] The nitrifying bacteria strains were inserted into the above-mentioned medium according to the inoculum amount of 10%, and the temperature was 30°C, and the culture was shaken at a constant temperature. After 12 hours of inoculation, the turbidity of nitrifying bacteria was significantly improved; however, the turbidity of the same batch was inoculated without adding glucose, vitamin solution, and zeolite, and the turbidity of the bacteria did not increase, but decreased slightly. The results show that the liquid culture medium provided by the invention p...

Embodiment 3

[0022] Add 0.5g of glucose, 1g of sodium nitrite, 0.01g of manganese sulfate monohydrate, 1g of potassium monohydrogen phosphate, 1g of sodium carbonate, 0.3g of potassium dihydrogen phosphate, 26.7g of sodium chloride, 3.3g of magnesium sulfate, and magnesium chloride in 1000ml of water 2.3g, calcium chloride 1.2g, sodium bicarbonate 0.2g, potassium chloride 0.75g, vitamin solution 2ml, trace element solution 2ml, zeolite 70g, pH=8.

[0023] The nitrifying bacteria strains were inserted into the above-mentioned medium according to the inoculum amount of 10%, and the temperature was 30°C, and the culture was shaken at a constant temperature. After 15 hours of inoculation, the turbidity of nitrifying bacteria was significantly improved; however, the turbidity of the same batch was inoculated to the medium without adding glucose, vitamin solution, and zeolite, and the turbidity of the bacteria did not increase, but decreased slightly. Experiments show that the liquid culture med...

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PUM

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Abstract

A fluid nutrient medium for promoting the growth of halophilic nitrite oxidizing bacteria contains 0.3g to 0.7g of glucose, 0.6g to 1.4g of sodium nitrite, 0g to 0.04g of manganese sulfate monohydrate, 0.6g to 1.5g of potassium monohydrogen phosphate, 0.6g to 1.4g of sodium carbonate, 0g to 0.6g of potassium dihydrogen phosphate, 20g to 32g of sodium chloride, 2.8g to 3.6g of magnesium sulfate, 1.8g to 2.6g of magnesium chloride, 0.8g to 1.4g of calcium chloride, 0g to 0.6g of sodium bicarbonate, 0.3g to 1.1g of potassium chloride, 1ml to 10ml of vitamin solution, 1ml to 10ml of trace element solution and 60g to 80g of zeolite per 1000 milliliter of water, and the pH value is 8. The fluid nutrient medium is suitable for the growth of halophilic nitrite oxidizing bacteria, and can promote the growth and metabolism of halophilic nitrite oxidizing bacteria. The fluid nutrient medium provided by the invention can promote the growth of halophilic nitrite oxidizing bacteria in the fluid nutrient medium, so that the separation, purification, reproduction and denitrifying capability detection of halophilic nitrite oxidizing bacteria can become simple and feasible.

Description

technical field [0001] The invention relates to a culture medium for salt-tolerant nitrifying bacteria, in particular to a liquid culture medium for effectively promoting the growth of salt-tolerant nitrifying bacteria. Background technique [0002] Nitrifying bacteria (Nitrite Oxidizing Bacteria) are mostly chemoautotrophic, which is the main bacterial group involved in nitrification in the process of biological denitrification. factor. Moreover, studies have shown that nitrifying bacteria are very sensitive to salinity, which increases the difficulty of biological treatment of high-salt wastewater. In addition, the long generation time and slow growth rate of autotrophic nitrifying bacteria make it difficult for nitrifying bacteria to grow in ordinary inorganic carbon source liquid medium. This makes it difficult to expand and cultivate the isolated and purified salt-tolerant nitrifying bacteria, and is difficult to apply to biological treatment of high-salt wastewate...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/00
Inventor 傅金祥鄂宏明唐婧张荣新唐玉兰马兴冠郜玉楠由昆
Owner SHENYANG JIANZHU UNIVERSITY
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