Cosmetic material composition
A technology of composition and cosmetics, applied in the direction of cosmetics, cosmetic preparations, dressing preparations, etc., can solve the problems of instability, irritation, low absorption, etc., to prevent skin aging, promote skin whitening, Effect of improving skin function
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experiment example 1
[0027] [Experimental Example 1] Procollagen Biosynthesis Ability
[0028] Fibroblasts isolated from human skin were implanted into 24 wells at a rate of 106 per well, and cultured until the growth reached 90%. After culturing it in serum-free medium for 24 hours, three single substances and four mixtures of Gynura procumbens, Gynura pseudochina and Averrhoa bilimbi were dissolved in serum-free medium treated at a concentration of 100ppm, and in CO 2 Cultured in the incubator for 24 hours. These supernatants were taken, and the increase or decrease of procollagen was verified with (I) type procollagen (Procollagen type 1) Elisa kit, and the results are shown in Table 1. Experiments were carried out at concentrations in consideration of cytotoxicity, and the synthetic ability was the result of comparison with the non-treated group as 100.
[0029] [Table 1]
[0030] Experimental substance
[0031] It can be seen from Table 1 that the single extract and the mixture ...
experiment example 2
[0032] [Experimental Example 2] Collagenase (MMP-1) inhibitory ability
[0033] In the experiment, 5000 cells / hole (well) of human fibroblasts were added to a 96-well plate culture device (96-well microtiter plate) containing DMEM (Dulbecco's Modified Eagle's Media) medium containing 2.5% fetal bovine serum, Cultivate until the growth is 70-80%. Then, three single substances and four mixtures of Gynura procumbens, Gynura pseudochina and Averrhoa bilimbi were treated at a concentration of 10ppm for 24 hours, and collected cell culture medium. The collected cell culture solution was used to measure the degree of collagenase production using a commercially available collagenase detection device (Amersham pharmacia, USA, Catalog #: RPN2610). First, the collected cell culture solution was added to a 96-well plate (96-well plate) uniformly coated with the collagenase antibody once, and an antigen-antibody reaction was carried out in an incubator for 3 hours. After 3 hours, the c...
experiment example 3
[0039] [Experimental Example 3] Evaluation of Melanin Production Inhibition Ability Using Melan-a Cells
[0040] Each of the produced extracts and mixtures was used as a test substance, and 2×10 4 Cells / well (final volume 3ml), to 96-well plate (MTT method) with 2×10 3 Cells / well (final volume: 200ul) were added, and cultured for 24 hours in a 37°C, 5% CO2 incubator (MCO-20AIC, Sanyo). After culturing, the medium was removed, and a DMEM medium containing 10% FBS, 2uM α-MSH (Melanocyte stimulating hormone) and 2mM theophylline was used as a new medium, and added to the medium The test substance was added and the treatment was carried out every day for 3 days. On day 5, the medium cultured in the 6-well plate was removed, and the pellet was recovered by treating with 0.25% trypsin-EDTA (Sigma Chemical Co.) solution, and moved to a 1.5 ml test tube (tube) for The supernatant was removed by centrifugation at 10000 rpm for 10 minutes. After drying the obtained pellet at 60°C, 1...
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