Lactobacillus johnsonii, microbial inoculum, application and premix thereof

A Lactobacillus johnsonii, premix technology, applied in application, bacteria, animal feed, etc., to achieve strong gastric acid tolerance, good stress resistance, and improve production performance.

Active Publication Date: 2012-04-04
BEIJING DABEINONG TECH GRP CO LTD +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The joint application of Lactobacillus johnsonii, Lactobacillus acidophilus and Bacillus pu

Method used

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  • Lactobacillus johnsonii, microbial inoculum, application and premix thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0021] Example 1 Verification of stress resistance of Lactobacillus johnsonii CGMCC No. 4926

[0022] Inoculate 1 mL of Lactobacillus johnsonii CGMCC No. 4926 in 10 mL of MRS medium, culture at 37°C for 20 hours, then take 3 mL of bacterial suspension, centrifuge at 12000 rpm for 5 minutes, discard the supernatant, and add the bacterial pellet In 10ml artificial gastric juice with pH2.0 and 1% pepsin, let stand for 2h at 37℃; centrifuge at 12000rpm for 5min, discard the supernatant, add the bacterial pellet to 10ml artificial bile salt with a concentration of 0.3%, and let stand at 37℃ for 3h . After shaking and mixing, take 1 mL of bacterial suspension and dilute gradiently with sterile normal saline on MRS medium plate. After anaerobic culture at 37°C for 20 hours, the colonies were purified. The strains obtained from the preliminary screening are further screened.

[0023] (1) Tolerance of artificial gastric juice

[0024] Take 1 mL of Lactobacillus johnsonii CGMCC No. 4926 ba...

Example Embodiment

[0031] Example 2 Verification of antibacterial ability of Lactobacillus johnsonii CGMCC No. 4926

[0032] Melt the LB solid medium and cool to 45°C. Add overnight cultured E.coli K88, K99 and Staphylococcus aureus (1 microliter of bacterial solution per ml of medium), shake and mix, pour into a sterile plate, solidify horizontally, and place gently on each agar plate 4 Oxford cups should be equally spaced. Add 50μL of Lactobacillus johnsonii bacterial solution to each Oxford cup, close the lid, and carefully move it to the 37°C incubator. The plate is placed in a static culture. After incubating for 20 hours, open the lid of the dish, remove the Oxford cup, and measure the diameter of the inhibition zone with a caliper (see Table 1 for the results).

[0033] Table 1 Determination of the zone of inhibition

[0034]

Example Embodiment

[0035] Example 3 Preparation of Lactobacillus johnsonii CGMCC No. 4926 viable preparation

[0036] Preparation of Lactobacillus johnsonii seed solution: inoculate the preserved strains of the test tube slope into an anaerobic tube containing 20 mL of MRS medium, and anaerobic culture at 37°C for 20 hours until the number of viable bacteria reaches 10 7 CFU / mL, inoculate 1% into a 250mL Erlenmeyer flask containing 100mL seed culture medium, and anaerobic culture at 37°C for 20h, until the number of viable bacteria reaches 10 7 CFU / mL or more, as a seed solution for later use.

[0037] Seed liquid MRS medium: peptone, 10.0g / L; beef extract, 8.0g / L; yeast extract, 4.0g / L; glucose, 20.0g / L; Tween 80, 1mL / L; dipotassium hydrogen phosphate, 2.0g / L; sodium acetate trihydrate, 5.0g / L; triammonium citrate, 2.0g / L; magnesium sulfate heptahydrate, 0.2g / L; manganese sulfate, 0.05g / L; distilled water to 1000mL, pH 6.2 ±0.2. Liquid submerged fermentation culture

[0038] Use a 100L vertical ferm...

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Abstract

The invention belongs to the technical field of microecological preparations, and particularly relates to lactobacillus johnsonii, a microbial inoculum, application and a premix thereof. The lactobacillus johnsonii, lactobacillus acidophilus and bacillus pumilus has a good health-benefiting function and high stress resistance; the three bacteria are prepared into a bacterial agent first and then into a feed additive to be added into the premix, and after animals are fed with the microbial inoculum and the feed additive, the productivity of weaned pigs can be improved obviously, the diarrhea rate of the pigs can be reduced, and the economic benefit can be improved. The lactobacillus johnsonii has a popularization and application prospect, and provides a theoretical and practice foundation for seeking antibiotic substitutes.

Description

technical field [0001] The invention belongs to the technical field of microecological preparations, and in particular relates to Lactobacillus johnsonii and its bacterial preparation, application and premix. Background technique [0002] As people's awareness of the negative effects of antibiotic feed additives continues to increase, feed microecological preparations, as a new type of antibiotic substitute, have many advantages such as no toxic side effects, no drug resistance, no residue, and significant effects. welcomed by the farming industry. [0003] As a kind of microecological preparation, compound live bacteria preparation has been widely used in animal husbandry. It is mainly composed of two or more different genera of probiotics, focusing on the complementarity between different genera, and generally exerting a positive combination effect. Probiotics with different biological functions are combined into a compound live bacterial preparation to maintain the stab...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K1/16C12R1/225C12R1/23C12R1/07
Inventor 胡婷刘滢赵莉莉刘婷
Owner BEIJING DABEINONG TECH GRP CO LTD
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