Preparation method of temperature-sensitive macroporous acrylamide/N-isopropyl acrylamide composite microsphere

A technology of isopropyl acrylamide and acrylamide, applied in the field of materials, can solve the problems of poor performance of immobilized enzymes, poor biocompatibility, shrinkage and deformation, etc., and achieve obvious macroporous structure and temperature sensitivity, The pore size is uniform and dense, and the effect of strong water absorption and adsorption

Inactive Publication Date: 2012-04-11
SHAANXI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these materials are either poorly biocompatible, hydrophobic, or prone to severe shrinkage and deformation, resulting in poor performance of the specified immobilized enzymes.

Method used

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  • Preparation method of temperature-sensitive macroporous acrylamide/N-isopropyl acrylamide composite microsphere
  • Preparation method of temperature-sensitive macroporous acrylamide/N-isopropyl acrylamide composite microsphere
  • Preparation method of temperature-sensitive macroporous acrylamide/N-isopropyl acrylamide composite microsphere

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1. Preparation of water phase

[0028] Add 1.0g of acrylamide, 4.0g of N-isopropylacrylamide, 0.40g of ammonium persulfate, and 1.0g of N,N'-methylenebisacrylamide into 25g of deionized water, and acrylamide and N-isopropylacrylamide The mass ratio of amide, ammonium persulfate, N, N'-methylenebisacrylamide, and deionized water is 1:4:0.4:1.0:25, stirred until the solid is completely dissolved, and prepared into 31.4 g of the water phase.

[0029] 2. Preparation of oil phase

[0030] Put 90g of cyclohexane in a 250mL three-necked flask equipped with a stirrer, add 0.1g of Span-60 and 0.5g of Span-80, and the mass ratio of Span-60 to Span-80 and cyclohexane is 1 : 5: 900, stir at room temperature until Span-60 and Span-80 are completely dissolved, and prepare 90.6 g of oil phase.

[0031] 3. Preparation of thermosensitive macroporous acrylamide / N-isopropylacrylamide composite microspheres

[0032] Get the aqueous phase 30g that step 1 prepares, add toluene 2g, mix uni...

Embodiment 2

[0044] In step 1 of preparing the water phase in Example 1, 1.0 g of acrylamide, 3.0 g of N-isopropylacrylamide, 0.5 g of ammonium persulfate, and 0.5 g of N, N'-methylenebisacrylamide were added to 25 g to remove In ionized water, the mass ratio of acrylamide to N-isopropylacrylamide, ammonium persulfate, N,N'-methylenebisacrylamide, and deionized water is 1:3:0.5:0.5:25, and stirred until solid Dissolve completely and prepare 30g of water phase. The other steps were the same as in Example 1 to prepare thermosensitive macroporous acrylamide / N-isopropylacrylamide composite microspheres.

Embodiment 3

[0046] In step 1 of preparing the water phase in Example 1, 1.0 g of acrylamide, 5.0 g of N-isopropylacrylamide, 0.25 g of ammonium persulfate, and 2.0 g of N, N'-methylenebisacrylamide were added to 25 g to remove In ionized water, the mass ratio of acrylamide to N-isopropylacrylamide, ammonium persulfate, N,N'-methylenebisacrylamide, and deionized water is 1:5:0.25:2.0:25, and stirred until solid Dissolve completely, and prepare 33.25g of water phase. The other steps were the same as in Example 1 to prepare thermosensitive macroporous acrylamide / N-isopropylacrylamide composite microspheres.

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Abstract

A preparation method of temperature-sensitive macroporous acrylamide / N-isopropyl acrylamide composite microspheres adopts toluene as a pore-forming agent; a water phase obtained by mixing acrylamide, N-isopropyl acrylamide, ammonium persulfate, N,N'-methylene diacrylamide, and deionized water is allowed to react with an oil phase obtained by mixing span-60, span-80, and cyclohexane so as to prepare acrylamide / N-isopropyl acrylamide composite microspheres. The preparation method of the invention is simple, has a low reaction temperature, and the prepared acrylamide / N-isopropyl acrylamide composite microspheres have an obvious macroporous structure, a uniform and dense pore size, a relatively large specific surface area, and strong water absorbability and adsorptivity.

Description

technical field [0001] The invention belongs to the technical field of materials, and in particular relates to temperature-sensitive macroporous acrylamide / N-isopropylacrylamide composite microspheres. Background technique [0002] The immobilized enzyme carrier material has a great influence on the properties of the enzyme, so the requirements for the type and performance of the carrier material are very strict. In general, the following points should be met: have functional groups that can react with enzymes; have a large specific surface area and porous structure; be insoluble in water; have very good mechanical rigidity and stability; and can prevent microbial degradation. In addition, it is also hoped that the carrier material is non-toxic, non-polluting, low in cost, and abundant in sources. [0003] Common immobilized enzyme carrier materials mainly include chitosan, sodium alginate and starch, glass, silica gel, aluminum, montmorillonite, and magnetic polymer micros...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08F220/54C08F220/56C08F222/38C08J9/28C12N11/08
Inventor 雷忠利张晶晶郝丽
Owner SHAANXI NORMAL UNIV
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