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Blood maintenance liquid, preparation method thereof and application thereof

A technology of maintenance fluid and blood, applied in application, preservation of human or animal body, animal husbandry, etc.

Inactive Publication Date: 2013-11-13
AFFILIATED HOSPITAL CHINA ACADEMY OF MILITARY MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In order to solve the defect that the existing blood maintenance solution only has the function of providing energy and anticoagulation, and has a large side effect on the blood (the nitric oxide content in the blood drops sharply, thereby causing insufficient blood supply to tissues or organs), the present invention provides a It can improve the hypoxic vasodilation function of banked blood, increase vasodilation activity during infusion, increase tissue perfusion flow, improve tissue ischemia and hypoxia (increase tissue blood supply and oxygen supply), thereby improving the safety and effectiveness of clinical blood transfusion blood care solution

Method used

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  • Blood maintenance liquid, preparation method thereof and application thereof
  • Blood maintenance liquid, preparation method thereof and application thereof
  • Blood maintenance liquid, preparation method thereof and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1-5

[0097] Embodiment 1-5, preparation of blood maintenance solution of the present invention

[0098] The formulation (1 L) of the blood maintenance solution of Examples 1-5 of the present invention is shown in Table 2.

[0099] Table 2 The formula of embodiment 1-5 blood maintenance liquid of the present invention

[0100]

[0101] The preparation method of the blood maintenance liquid of the present invention comprises the following steps:

[0102] 1) Weigh each component according to the above formula;

[0103] 2) Mix the components in step 1) evenly, dissolve them with medical water, adjust the volume to 5.5, and obtain the blood maintenance solution.

[0104] The method of using the blood maintenance solution of the present invention is as follows: mix 14mL of the blood maintenance solution with 100±10mL of collected blood, and constantly shake and mix evenly during the blood collection process to ensure that the blood maintenance solution is fully mixed with the blood....

Embodiment 6

[0105] Embodiment 6. Detecting the influence of the blood maintenance solution of the present invention on the vasodilation function

[0106] The experiment is divided into two groups: 1. The blood maintenance solution of Example 1-5 of the present invention is the experimental group, and the corresponding final concentrations of L-arginine are 2.2, 2.4, 2.6, 2.8, 3.0mmol / L; 2. CPD-A maintenance solution (purchased from Weigao Biotechnology Co., Ltd.) was used as the control group. Mix 14mL of maintenance solution with 100±10mL of collected blood. During the blood collection process, keep shaking and mixing to ensure that the blood maintenance solution and blood are fully mixed. The method in Experimental Example 3 was used to detect the effect of the blood maintenance solution of the present invention on the vasodilation function.

[0107] The results are shown in Table 3. After analysis of variance, the effect of each experimental group on the vasodilation function was stat...

Embodiment 7

[0110] Embodiment 7, detecting the blood storage time of the blood maintenance solution of the present invention

[0111]Taking the commercially available blood maintenance solution CPDA as a contrast (control group), the blood storage time of the blood maintenance solution of the present invention (L-arg group, represented by Example 3) was detected. 14mL of blood maintenance solution was mixed with 100±10mL of collected blood and put into the warehouse for storage) when storing for 0d, 7d, 14d, 21d, 28d, and 35d, the blood storage period and quality after adding the blood maintenance solution of the present invention were preliminarily evaluated and tested. Blood pH, K + 、Na + , Cl - , SO 2 (blood oxygen saturation), PO 2 (partial pressure of oxygen), PCO 2 (partial pressure of carbon dioxide), HCO 3 - , ME (lactic acid), red blood cell deformability and aggregation, the specific results are as follows Figure 7-Figure 18 As shown, the result is compared with the con...

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Abstract

The invention discloses a blood maintenance liquid and a preparation method thereof. The blood maintenance liquid is an aqueous solution comprising 88.85-90.00mmol / L of sodium citrate, 15.10-16.20mmol / L of citric acid, 136.50-146.60mmol / L of waterless glucose, 14.00-14.31mmol / L of sodium dihydrogen phosphate (NaH2PO4.H2O), 5.30-5.58mmol / L of adenine and 2.20-3.00mmol / L of L-arginine, wherein the pH value of the aqueous solution is 5.5. According to the invention, a case that the blood maintenance liquid is only used for maintaining the oxygen carrying function of erythrocytes is fundamentally changed, and new functions of hemangiectasis and local blood amount increase are endowed to stored blood, so the vasodilation activity is improved, the tissue perfusion flow is increased and the tissue blood and oxygen supply is increased during blood transfusion, thereby the blood maintenance liquid of the invention, which allows the clinical blood transfusion security and validity to be improved, is a revolution of the blood preservation and the blood quality control.

Description

technical field [0001] The invention belongs to blood maintenance liquid in the field of blood preservation, its preparation method and application. Background technique [0002] With the continuous improvement of medical technology in our country, the amount of blood transfusion is also increasing year by year. In 2006, the amount of blood used in our country was 1,600 tons, and in 2009 it reached 3,000 tons. Blood transfusion plays an important role in treating blood loss and anemia patients. At present, the main blood maintenance solutions used are ACD, CPD or CPDA, and their main function is to anticoagulate and provide energy for red blood cells. However, some studies have shown that blood transfusion poses a great threat to the survival and health of patients, and blood transfusion can cause severe ischemia, hypoxia and reperfusion injury to local organ tissues in critically ill patients, especially in patients with cardiac insufficiency. more obvious. The main reas...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02
Inventor 王全立冯双利陆颖韩颖杜为陈民才陈廷友卓海龙
Owner AFFILIATED HOSPITAL CHINA ACADEMY OF MILITARY MEDICAL SCI
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