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Gene-releasing type stent and preparation method thereof

A gene and substrate technology, applied in the field of gene-releasing stents and its preparation, can solve the problems of low drug loading, small loading, and complicated preparation process, and achieve good controllability, rapid release, and simple operation

Inactive Publication Date: 2012-05-09
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Its shortcoming is that the preparation process of the scaffold is complicated. It utilizes the adsorption of mercaptoalkanol and drug or gene to realize drug loading, and uses ethanol solution for fixation. control
Its disadvantage is that the scaffold uses biodegradable polymer materials to load genes, and its load is very small; the genes carried by it are naked genes, and its entry into target cells and transfection efficiency in target cells are very low

Method used

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  • Gene-releasing type stent and preparation method thereof
  • Gene-releasing type stent and preparation method thereof
  • Gene-releasing type stent and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Such as figure 1 , 2As shown, the present invention consists of a bare metal stent 1, a substrate layer 2 and a gene-carrying coating 3, and the substrate layer 2 consists of 2 layers of substrate polyanion carrier layer 4 (polyacrylic acid layer) and 1 layer of substrate polycation carrier Layer 5 (polyethyleneimine layer) is composed alternately. The gene-carrying coating 3 is composed of 3 layers of biodegradable polycation carrier 6 (polyurethane) and 3 layers of gene layer 7 (plasmid DNA layer) alternately. Electrostatic attraction is combined with each other.

[0037] The preparation method comprises the following steps:

[0038] (1) Preparation of polyacrylic acid solution: dissolve polyacrylic acid in sodium chloride solution, and the concentration of polyacrylic acid in the prepared solution is 2 mg / mL;

[0039] (2) Preparation of polyethyleneimine solution: dissolve polyethyleneimine in sodium chloride solution, and the concentration of polyethyleneimine in...

Embodiment 2

[0047] Such as figure 1 , 2 As shown, the present invention consists of a bare metal stent 1, a substrate layer 2 and a gene-carrying coating 3, and the substrate layer 2 consists of 16 layers of substrate polyanion carrier layer 4 (polystyrene sulfonic acid layer) and 15 layers of substrate The polycationic carrier layer 5 (polyethyleneimine layer) is composed alternately, and the gene-carrying coating layer 3 is composed of 20 biodegradable polycationic carrier layers 6 (polyurethane) and 20 gene layers 7 alternately, and the above layers are separated by static electricity. The attraction is combined with each other, the gene layer is composed of genes and gene complexes, and the gene complexes are composed of genes (DNA) and polycationic lipids.

[0048] The preparation method comprises the following steps:

[0049] (1) Preparation of polystyrene sulfonic acid solution: dissolve polystyrene sulfonic acid in sodium chloride solution, and the concentration of polystyrene s...

Embodiment 3

[0058] Such as figure 1 , 2 As shown, the present invention consists of a bare metal stent 1, a substrate layer 2 and a gene-carrying coating 3, and the substrate layer 2 consists of a substrate polyanion carrier layer 4 (a mixed layer of polyacrylamide and polyvinyl phosphoric acid) and 1 layer of substrate polycationic carrier layer 5 (mixed layer of chitin and polyarginine) is composed alternately, and the gene-carrying coating layer 3 is composed of 1 layer of biodegradable polycationic carrier layer 6 (polyphosphoramide) and 1 layer of gene Layer 7 is composed alternately, and the above layers are combined with each other by electrostatic attraction. The gene layer is composed of genes and gene complexes. The gene complex is composed of genes (SiRNA), polyvinylamine and polyvinylpyridine.

[0059] The preparation method comprises the following steps:

[0060] (1) Preparation of a mixed solution of polyacrylamide and polyvinyl phosphoric acid: dissolve polyacrylamide and...

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Abstract

The invention relates to a gene-releasing type stent and a preparation method thereof. The stent comprises a bare metal stent, a substrate layer and a gene-loading coating, wherein the substrate layer is formed by alternating two layers or more substrate polyanion carrier layers and polycation carrier layers; the gene-loading coating is formed by alternating two layers or more biodegradable polycation carrier layers and gene layers; and all layers are mutually combined by electrostatic attraction. The preparation method for the gene-releasing type stent is characterized in that the metal stent is repeatedly and alternatively dipped into a polycation carrier solution and a gene solution with a solution dipping method, and therefore, the carrier layers and the gene layers can be alternatively coated on the surface of the stent. The stent disclosed by the invention is used for loading all genes (comprising DNA (Deoxyribo Nucleic Acid) and RNA (Ribose Nucleic Acid)) with negative charges to slowly release or quickly release gene drug. After the gene-releasing type stent is implanted in a human body, the gene-releasing type stent not only can mechanically expand a narrow channel but also can release gene therapy drug to the lesion tube wall tissue direction. The gene-releasing type stent is used for treating benign or malignant stenosis diseases of the physiological channel of the human body.

Description

technical field [0001] The invention relates to a bracket in the technical field of medical devices and a preparation method thereof, in particular to a gene release type bracket and a preparation method thereof. Background technique [0002] Stenosis of human physiological channels or lumens, especially benign and malignant stenosis or obstruction of blood vessels and digestive tracts, is a common serious clinical disease. In addition to surgical resection, radiotherapy, and chemotherapy, commonly used interventional methods include balloon dilatation (mainly for benign strictures) and stent placement. Among them, stent placement is a very important treatment method, which can effectively improve the quality of life of patients and prolong survival time. Clinical stent implantation is based on interventional surgery. A tube made of metal or other materials is placed in the obstructed or narrow physiological pipeline in the human body, and the mechanical force of the stent ...

Claims

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Application Information

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IPC IPC(8): A61L31/16A61L31/10A61F2/82
Inventor 郭圣荣雷磊沈园园荣浩钧陈伟峦
Owner SHANGHAI JIAO TONG UNIV
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