Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Contagious caprine plueropneumonia antibody detection test strips, and preparation method thereof

A technology for antibody detection and pleuropneumonia, applied in measuring devices, instruments, scientific instruments, etc., can solve problems such as oversensitivity, false positives, and poor sensitivity, and achieve the effect of reducing economic losses

Inactive Publication Date: 2012-05-09
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
View PDF5 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above diagnostic methods have played a certain effective role in the prevention and treatment of Mccp, but there are still deficiencies: Complement fixation test: Mccp infection is determined by the complement fixation test, which is a designated test for international trade
However, the sensitivity is poorer than ELISA and gold standard diagnostic methods
Competitive enzyme-linked immunosorbent assay: Although this method can be effectively used for long-term antibody detection after Mccp infection (Thiaucourt, Bolske, Libeau, LeGoff, & Lefevre, 1994; Sharew, 2005), its sensitivity is too high, often causing false positives

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Contagious caprine plueropneumonia antibody detection test strips, and preparation method thereof
  • Contagious caprine plueropneumonia antibody detection test strips, and preparation method thereof
  • Contagious caprine plueropneumonia antibody detection test strips, and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1. Preparation of colloidal gold: Colloidal gold was prepared by trisodium citrate reduction method.

[0027] 2. Preparation of colloidal gold-labeled polysaccharide antigen: firstly, use 0.1mol / L KCO 3 Adjust the pH value to 6.0; then determine the optimal amount of colloidal gold and the polysaccharide antigen to be labeled by visual inspection, and obtain the optimal labeling amount of 1mg / 100ml, add Mycoplasma goat pneumoniae sub A polysaccharide antigen, let it stand for 30min; centrifuge the above colloidal gold at 2500r / min for 30min, remove the precipitate, and obtain a supernatant; centrifuge the supernatant at 10000r / min for 30min, discard the supernatant to collect the precipitate, and the precipitate Suspend in gold colloid buffer that is 1 / 10 times the volume of initial colloidal gold, and store at 4°C; gold colloid buffer is phosphate buffer containing 5‰ sucrose, 1% BSA, 0.5‰ PEG20000, 0.5‰ Tween-20 , concentration 0.02mol / L, pH7.4.

[0028] 3. Assembly o...

Embodiment 2

[0033] 1. Preparation of colloidal gold: Colloidal gold was prepared by trisodium citrate reduction method;

[0034] 2. Preparation of colloidal gold-labeled polysaccharide antigen: first, use 0.1mol / L KCO 3 Adjust the pH value to 7.0; then determine the optimal amount of colloidal gold and the polysaccharide antigen to be labeled by visual inspection, and obtain the optimal labeling amount of 3.4mg / 100ml, add Mycoplasma capricosum to the colloidal gold solution at 3.4mg / 100ml Pneumonia subspecies polysaccharide antigen, let it stand for 30min; centrifuge the above colloidal gold at 2500r / min for 30min, remove the precipitate, and obtain the supernatant; centrifuge the supernatant at 10000r / min for 30min, discard the supernatant to collect the precipitate, and Suspend the precipitate in gold colloidal buffer that is 1 / 10 times the volume of the initial colloidal gold, and store at 4°C; the gold colloid buffer is phosphate containing 15‰ sucrose, 1% BSA, 1‰ PEG20000, 1‰ Tween-2...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention relates to contagious caprine plueropneumonia (CCPP) antibody detection test strips. The test strips comprise: a PVC liner, a nitrocellulose membrane, an absorption pad, a gold standard pad and a sample pad. The left end of the nitrocellulose membrane is sprayed with anti-mycoplasma capricolum subsp. capripneumoniae mccp IgG, wherein the IgG is adopted as a quality control line. The right end of the nitrocellulose membrane is sprayed with mycoplasma capricolum subsp. capripneumoniae mccp polysaccharide antigen, wherein the polysaccharide antigen is adopted as a detection line. The gold standard pad is sprayed with colloidal gold-labeled mycoplasma capricolum subsp. capripneumoniae mccp polysaccharide antigen. The preparation process comprises: a, preparing the colloidal gold, wherein a sodium citrate reduction method is adopted to prepare the colloidal gold; b, preparing the colloidal gold-labeled mycoplasma capricolum subsp. capripneumoniae mccp polysaccharide antigen; c, assembling the antibody detection test strip. According to the present invention, the presences of the detection line and the quality control line are adopted to determine whether the CCPP antibody exists; the test strips of the present invention have characteristics of convenient detection, fastness and accuracy, and are applicable for primary level quarantine, epidemiological investigation quarantine, isolated quarantine and rapid detection so as to eliminate infected sheep in time, control disease occurrence, and reduce economic losses.

Description

technical field [0001] The invention belongs to the technical field of animal inspection and detection, and relates to the field of rapid and accurate diagnosis of goat contact infectious pleuropneumonia antibody, specifically a test strip for detection of goat contact infectious pleuropneumonia antibody, and the invention also includes the test strip Preparation. Background technique [0002] Goat contact infectious pleuropneumonia (contagious caprine pleuropneumonia CCPP) is caused by Mycoplasma caprine caprine pneumonia subspecies ( Mycoplasma capricolum subsp. capripneumoniae McCp) is a highly contagious disease. The International Veterinary Office (OIE) classifies it as a category B infectious disease of sheep. Up to now, more than 40 countries including Sudan, India, Pakistan, Turkey, Syria, Kenya and Ethiopia have reported the occurrence of the disease (OIE, 2008). In my country, in 1935, the "rotten lung disease" of goats was prevalent in the Bailingmiao ar...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N33/558G01N33/531
Inventor 逯忠新高鹏程储岳峰赵萍贺英高珊
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com