Green integrated treatment method for food waste
A technology of food waste and treatment methods, applied in the direction of biofuel, waste fuel, solid waste removal, etc., can solve the problems of polluting the environment, long cycle, occupying land, etc.
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Embodiment 1
[0018] Embodiment 1 fermentation obtains microbial fat
[0019] Specific steps:
[0020] 1) Strain activation: activate the preserved slant at 28°C for 12 hours;
[0021] 2) Scratch a ring with an inoculation needle. Step 1) Inoculate the activated slant bacteria into 50 mL of sterile seed liquid culture medium, shake and culture at 25°C and 140 rpm for 30 hours to obtain seed liquid;
[0022] 3) Fermentation culture: Inoculate the seed culture solution obtained in step 2) into the wastewater fermentation medium at an inoculum size of 10% (V / V), and cultivate for 120 hours under shaking at 25° C. and 150 rpm;
[0023] 4) Post-fermentation treatment: After the fermentation is finished, the fermented bacterial cells are collected by centrifugation, dried and ground, and the microbial oil is extracted by the Soxhlet extraction method (GB / T 5009.6-1985).
[0024] After testing, the oil content obtained by the above method accounts for 10-30% of the dry weight of the cells.
Embodiment 2
[0025] Embodiment 2 gained oil conversion synthesis biodiesel
[0026] Specific steps: 2g microbial oil or kitchen waste oil or any mixture of the two, 4g lipase cloth (high-spun fabric carrier immobilized lipase, apparent enzyme activity 20000-25000U / g), 200μl deionized water, 2ml petroleum Ether and 93 μl of methanol (or ethanol) were reacted at 40°C and 180 rpm in a closed shaker for 12 hours, and samples were taken and analyzed at regular intervals. 93 μl of methanol (or ethanol) was added every four hours (three times in total). After the reaction, the reaction solution was diluted 100 times and then used for gas chromatography analysis.
[0027] After testing, the conversion rate of the oil converted into biodiesel obtained by the above method is 85%-95%.
Embodiment 3
[0028] Embodiment 3 gained oil conversion synthesis biodiesel
[0029] Specific steps: after taking enough food waste at one time, remove impurities such as bones, fishbone, napkins, etc., crush it with a small crusher, use a 1L jar for anaerobic fermentation, and do 3 groups of parallel experiments at the same time, and ferment for 25 days The biogas production rate was monitored during the process.
[0030] After testing, the daily biogas output obtained by the above method is in the range of 150ml / g-210ml / g.
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